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Field	Query

	Field	Term

General Information:

Id:	4,064
Organism:	Mus musculus
Strain:	PepBoy (B6.SJL-PtprcaPepcb/BoyJ), and B6 (C57BL/6J), 6 to 8 wk-old; RelA(p65):Vav-Cre = p65(hem-/-)
Reference:	Stein SJ and Baldwin AS(2013) Deletion of the NF-kappaB subunit p65/RelA in the hematopoietic compartment leads to defects in hematopoietic stem cell function Blood 121: 5015-5024 [PMID: 23670180]

Interaction Information:

Comment	Deletion of p65 (RelA) in hematopoietic cells results in an increase of KSL, CD34-CD135-KSL and CD34+CD135-KSL cells, whereas the frequency of CD34+CD135+KSL cells did not change. [Method: p65(hem-/-), FACS]
Formal Description Interaction-ID: 41879	gene/protein Rela decreases_quantity of Immunophenotype HSC (CD34-CD135-KSL) in BM in vivo
Drugbank entries	Show/Hide entries for Rela
Drugbank DB02836	Guanosine 5'-Diphosphate 2':3'-Cyclic Mo ... not specified relA
Drugbank DB04315	Guanosine-5'-Diphosphate not specified relA
Drugbank DB02836	Guanosine 5'-Diphosphate 2':3'-Cyclic Mo ... not specified relA
Drugbank DB04315	Guanosine-5'-Diphosphate not specified relA

Comment	Deletion of p65 (RelA) in hematopoietic cells results in an increase of KSL, CD34-CD135-KSL and CD34+CD135-KSL cells, whereas the frequency of CD34+CD135+KSL cells did not change. [Method: p65(hem-/-), FACS]
Formal Description Interaction-ID: 41880	gene/protein Rela decreases_quantity of Immunophenotype HSC (CD34+CD135-KSL) in BM in vivo
Drugbank entries	Show/Hide entries for Rela
Drugbank DB02836	Guanosine 5'-Diphosphate 2':3'-Cyclic Mo ... not specified relA
Drugbank DB04315	Guanosine-5'-Diphosphate not specified relA
Drugbank DB02836	Guanosine 5'-Diphosphate 2':3'-Cyclic Mo ... not specified relA
Drugbank DB04315	Guanosine-5'-Diphosphate not specified relA

Comment	Deletion of p65 (RelA) in hematopoietic cells results in an decrease of CMPs, GMPs, MEPs, CLPs. [Method: p65(hem-/-), FACS]
Formal Description Interaction-ID: 41881	gene/protein Rela increases_quantity of Immunophenotype CMP (CD34+CD16/32-Kit+Sca1-Lin-) in BM in vivo
Drugbank entries	Show/Hide entries for Rela
Drugbank DB02836	Guanosine 5'-Diphosphate 2':3'-Cyclic Mo ... not specified relA
Drugbank DB04315	Guanosine-5'-Diphosphate not specified relA
Drugbank DB02836	Guanosine 5'-Diphosphate 2':3'-Cyclic Mo ... not specified relA
Drugbank DB04315	Guanosine-5'-Diphosphate not specified relA

Comment	Deletion of p65 (RelA) in hematopoietic cells results in an decrease of CMPs, GMPs, MEPs, CLPs. [Method: p65(hem-/-), FACS]
Formal Description Interaction-ID: 41882	gene/protein Rela increases_quantity of Immunophenotype GMP (CD34+CD16/32+Kit+Sca1-Lin-) in BM in vivo
Drugbank entries	Show/Hide entries for Rela
Drugbank DB02836	Guanosine 5'-Diphosphate 2':3'-Cyclic Mo ... not specified relA
Drugbank DB04315	Guanosine-5'-Diphosphate not specified relA
Drugbank DB02836	Guanosine 5'-Diphosphate 2':3'-Cyclic Mo ... not specified relA
Drugbank DB04315	Guanosine-5'-Diphosphate not specified relA

Comment	Deletion of p65 (RelA) in hematopoietic cells results in an decrease of CMPs, GMPs, MEPs, CLPs. [Method: p65(hem-/-), FACS]
Formal Description Interaction-ID: 41883	gene/protein Rela increases_quantity of Immunophenotype MEP (CD34-CD16/32-Kit+Sca1-Lin-) in BM in vivo
Drugbank entries	Show/Hide entries for Rela
Drugbank DB02836	Guanosine 5'-Diphosphate 2':3'-Cyclic Mo ... not specified relA
Drugbank DB04315	Guanosine-5'-Diphosphate not specified relA
Drugbank DB02836	Guanosine 5'-Diphosphate 2':3'-Cyclic Mo ... not specified relA
Drugbank DB04315	Guanosine-5'-Diphosphate not specified relA

Comment	Deletion of p65 (RelA) in hematopoietic cells results in an decrease of CMPs, GMPs, MEPs, CLPs. [Method: p65(hem-/-), FACS]
Formal Description Interaction-ID: 41884	gene/protein Rela increases_quantity of Immunophenotype CLP in BM in vivo
Drugbank entries	Show/Hide entries for Rela
Drugbank DB02836	Guanosine 5'-Diphosphate 2':3'-Cyclic Mo ... not specified relA
Drugbank DB04315	Guanosine-5'-Diphosphate not specified relA
Drugbank DB02836	Guanosine 5'-Diphosphate 2':3'-Cyclic Mo ... not specified relA
Drugbank DB04315	Guanosine-5'-Diphosphate not specified relA

Comment	The percentage of CD135-KSL cells increased by threefold in the bone marrow of animals receiving p65-/- BM cells. Deletion of p65 impairs HSC repopulation ability in vivo. [Method: competitive transplantation: BM cells from p65(hem-/-) mice transplanted into recipients (PepBoy, 6-8 wks-old), 20 wks posttransplant]
Formal Description Interaction-ID: 41885	gene/protein Rela increases_activity of process repopulation >16 weeks of Cd135-KSL cells in vivo
Drugbank entries	Show/Hide entries for Rela
Drugbank DB02836	Guanosine 5'-Diphosphate 2':3'-Cyclic Mo ... not specified relA
Drugbank DB04315	Guanosine-5'-Diphosphate not specified relA
Drugbank DB02836	Guanosine 5'-Diphosphate 2':3'-Cyclic Mo ... not specified relA
Drugbank DB04315	Guanosine-5'-Diphosphate not specified relA

Comment	The percentage of CD135-KSL cells increased by threefold in the bone marrow of animals receiving p65-/- BM cells. Deletion of p65 impairs HSC repopulation ability in vivo. [Method: competitive transplantation: BM cells from p65(hem-/-) mice transplanted into recipients (PepBoy, 6-8 wks-old), 20 wks posttransplant]
Formal Description Interaction-ID: 41886	process I-kappaB kinase/NF-kappaB signaling increases_activity of process repopulation >16 weeks of Cd135-KSL cells in vivo

Comment	RelA (p65) is a known player of NF-kappaB signaling. [Method: cited information]
Formal Description Interaction-ID: 41887	gene/protein Rela increases_activity of process I-kappaB kinase/NF-kappaB signaling
Drugbank entries	Show/Hide entries for Rela
Drugbank DB02836	Guanosine 5'-Diphosphate 2':3'-Cyclic Mo ... not specified relA
Drugbank DB04315	Guanosine-5'-Diphosphate not specified relA
Drugbank DB02836	Guanosine 5'-Diphosphate 2':3'-Cyclic Mo ... not specified relA
Drugbank DB04315	Guanosine-5'-Diphosphate not specified relA

Comment	IKKbeta functions as an activator of NF-kappaB signaling. [Method: cited information]
Formal Description Interaction-ID: 41941	gene/protein Ikbkb increases_activity of process I-kappaB kinase/NF-kappaB signaling in vivo
Drugbank entries	Show/Hide entries for Ikbkb
Drugbank DB00244	Mesalazine inhibitor IKBKB
Drugbank DB00795	Sulfasalazine inhibitor IKBKB
Drugbank DB00995	Auranofin inhibitor IKBKB
Drugbank DB01169	Arsenic trioxide inducer IKBKB

Comment	Spleens of p65(hem-/-) showed an increase in Cd135-KSL cells. [Method: spleens of p65(hem-/-) mice, average number of CD135-KSL cells]
Formal Description Interaction-ID: 41947	gene/protein Rela decreases_quantity of Immunophenotype HSC (CD135-KSL) in the spleen in vivo
Drugbank entries	Show/Hide entries for Rela
Drugbank DB02836	Guanosine 5'-Diphosphate 2':3'-Cyclic Mo ... not specified relA
Drugbank DB04315	Guanosine-5'-Diphosphate not specified relA
Drugbank DB02836	Guanosine 5'-Diphosphate 2':3'-Cyclic Mo ... not specified relA
Drugbank DB04315	Guanosine-5'-Diphosphate not specified relA

Comment	Spleens of p65(hem-/-) showed an increase in Cd135-KSL cells. [Method: spleens of p65(hem-/-) mice, average number of CD135-KSL cells]
Formal Description Interaction-ID: 41953	gene/protein Rela decreases_activity of process extramedullary hematopoiesis in vivo
Drugbank entries	Show/Hide entries for Rela
Drugbank DB02836	Guanosine 5'-Diphosphate 2':3'-Cyclic Mo ... not specified relA
Drugbank DB04315	Guanosine-5'-Diphosphate not specified relA
Drugbank DB02836	Guanosine 5'-Diphosphate 2':3'-Cyclic Mo ... not specified relA
Drugbank DB04315	Guanosine-5'-Diphosphate not specified relA

Comment	Spleens of p65(hem-/-) showed an increase in Cd135-KSL cells. [Method: spleens of p65(hem-/-) mice, average number of CD135-KSL cells]
Formal Description Interaction-ID: 41954	gene/protein Rela decreases_activity of disease Splenomegaly in vivo
Drugbank entries	Show/Hide entries for Rela
Drugbank DB02836	Guanosine 5'-Diphosphate 2':3'-Cyclic Mo ... not specified relA
Drugbank DB04315	Guanosine-5'-Diphosphate not specified relA
Drugbank DB02836	Guanosine 5'-Diphosphate 2':3'-Cyclic Mo ... not specified relA
Drugbank DB04315	Guanosine-5'-Diphosphate not specified relA

Comment	Deletion of p65 (Rela) does not affect the homing efficiency of hematopoietic cells. [Method: BM cells from p65(hem-/-) mice, injected into the lateral tail vein of lethally irradiated mice. Engraftment of donor cells analyzed 48 hours after transplant]
Formal Description Interaction-ID: 41960	gene/protein Rela NOT affects_activity of process homing in vivo
Drugbank entries	Show/Hide entries for Rela
Drugbank DB02836	Guanosine 5'-Diphosphate 2':3'-Cyclic Mo ... not specified relA
Drugbank DB04315	Guanosine-5'-Diphosphate not specified relA
Drugbank DB02836	Guanosine 5'-Diphosphate 2':3'-Cyclic Mo ... not specified relA
Drugbank DB04315	Guanosine-5'-Diphosphate not specified relA

Comment	Deletion of p65 (RelA) decreases apoptosis in HSPCs. [Method: HSC (CD34-CD135-KSL) from p65(hem-/-) mice, Annexin V staining]
Formal Description Interaction-ID: 41963	gene/protein Rela increases_activity of process apoptotic process in HSC (CD34-CD135-KSL) in vivo
Drugbank entries	Show/Hide entries for Rela
Drugbank DB02836	Guanosine 5'-Diphosphate 2':3'-Cyclic Mo ... not specified relA
Drugbank DB04315	Guanosine-5'-Diphosphate not specified relA
Drugbank DB02836	Guanosine 5'-Diphosphate 2':3'-Cyclic Mo ... not specified relA
Drugbank DB04315	Guanosine-5'-Diphosphate not specified relA

Comment	Deletion of p65 (RelA) results in differential expression of genes involved in HSC fate. [Method: HSC (CD135-KSL) from p65(hem-/-) mice, qRT-PCR]
Formal Description Interaction-ID: 41964	gene/protein Rela increases_expression of gene/protein Hlf in HSC (CD135-KSL)
Drugbank entries	Show/Hide entries for Rela
Drugbank DB02836	Guanosine 5'-Diphosphate 2':3'-Cyclic Mo ... not specified relA
Drugbank DB04315	Guanosine-5'-Diphosphate not specified relA
Drugbank DB02836	Guanosine 5'-Diphosphate 2':3'-Cyclic Mo ... not specified relA
Drugbank DB04315	Guanosine-5'-Diphosphate not specified relA

Comment	Deletion of p65 (RelA) results in differential expression of genes involved in HSC fate. [Method: HSC (CD135-KSL) from p65(hem-/-) mice, qRT-PCR]
Formal Description Interaction-ID: 41968	gene/protein Rela increases_expression of gene/protein Mpl in HSC (CD135-KSL)
Drugbank entries	Show/Hide entries for Rela
Drugbank DB02836	Guanosine 5'-Diphosphate 2':3'-Cyclic Mo ... not specified relA
Drugbank DB04315	Guanosine-5'-Diphosphate not specified relA
Drugbank DB02836	Guanosine 5'-Diphosphate 2':3'-Cyclic Mo ... not specified relA
Drugbank DB04315	Guanosine-5'-Diphosphate not specified relA

Comment	Deletion of p65 (RelA) results in differential expression of genes involved in HSC fate. [Method: HSC (CD135-KSL) from p65(hem-/-) mice, qRT-PCR]
Formal Description Interaction-ID: 41970	gene/protein Rela decreases_expression of gene/protein Met in HSC (CD135-KSL)
Drugbank entries	Show/Hide entries for Rela or Met
Drugbank DB02836	Guanosine 5'-Diphosphate 2':3'-Cyclic Mo ... not specified relA
Drugbank DB04315	Guanosine-5'-Diphosphate not specified relA
Drugbank DB02836	Guanosine 5'-Diphosphate 2':3'-Cyclic Mo ... not specified relA
Drugbank DB04315	Guanosine-5'-Diphosphate not specified relA
Drugbank DB02152	K-252a not specified MET
Drugbank DB06896	1-(4-fluorophenyl)-N-[3-fluoro-4-(1H-pyr ... not specified MET
Drugbank DB06995	N-({4-[(2-aminopyridin-4-yl)oxy]-3-fluor ... not specified MET
Drugbank DB06997	2-(4-fluorophenyl)-N-{[3-fluoro-4-(1H-py ... not specified MET
Drugbank DB07369	N-(3-chlorophenyl)-N-methyl-2-oxo-3-[(3, ... not specified MET
Drugbank DB07969	3-[3-(4-methylpiperazin-1-yl)-7-(trifluo ... not specified MET
Drugbank DB08584	6-{[6-(1-methyl-1H-pyrazol-4-yl)[1,2,4]t ... not specified MET
Drugbank DB08700	3-[(1R)-1-(2,6-dichloro-3-fluorophenyl)e ... not specified MET
Drugbank DB08791	1-[(2-NITROPHENYL)SULFONYL]-1H-PYRROLO[3 ... not specified MET
Drugbank DB02152	K-252a not specified MET

Comment	Deletion of p65 (RelA) results in differential expression of genes involved in HSC fate. [Method: HSC (CD135-KSL) from p65(hem-/-) mice, qRT-PCR]
Formal Description Interaction-ID: 41971	gene/protein Rela decreases_expression of gene/protein Csf1r in HSC (CD135-KSL)
Drugbank entries	Show/Hide entries for Rela or Csf1r
Drugbank DB02836	Guanosine 5'-Diphosphate 2':3'-Cyclic Mo ... not specified relA
Drugbank DB04315	Guanosine-5'-Diphosphate not specified relA
Drugbank DB02836	Guanosine 5'-Diphosphate 2':3'-Cyclic Mo ... not specified relA
Drugbank DB04315	Guanosine-5'-Diphosphate not specified relA
Drugbank DB00619	Imatinib antagonist CSF1R
Drugbank DB01268	Sunitinib other/unknown CSF1R
Drugbank DB07167	5-CYANO-FURAN-2-CARBOXYLIC ACID [5-HYDRO ... not specified CSF1R
Drugbank DB07202	6-CHLORO-3-(3-METHYLISOXAZOL-5-YL)-4-PHE ... not specified CSF1R

Comment	Deletion of p65 (RelA) results in differential expression of genes involved in HSC fate. [Method: HSC (CD135-KSL) from p65(hem-/-) mice, qRT-PCR]
Formal Description Interaction-ID: 41972	gene/protein Rela decreases_expression of gene/protein Igsf6 in HSC (CD135-KSL)
Drugbank entries	Show/Hide entries for Rela
Drugbank DB02836	Guanosine 5'-Diphosphate 2':3'-Cyclic Mo ... not specified relA
Drugbank DB04315	Guanosine-5'-Diphosphate not specified relA
Drugbank DB02836	Guanosine 5'-Diphosphate 2':3'-Cyclic Mo ... not specified relA
Drugbank DB04315	Guanosine-5'-Diphosphate not specified relA

Comment	Deletion of p65 (RelA) results in differential expression of genes involved in HSC fate. [Method: HSC (CD135-KSL) from p65(hem-/-) mice, qRT-PCR]
Formal Description Interaction-ID: 41973	gene/protein Rela decreases_expression of gene/protein Ly6c1 in HSC (CD135-KSL)
Drugbank entries	Show/Hide entries for Rela
Drugbank DB02836	Guanosine 5'-Diphosphate 2':3'-Cyclic Mo ... not specified relA
Drugbank DB04315	Guanosine-5'-Diphosphate not specified relA
Drugbank DB02836	Guanosine 5'-Diphosphate 2':3'-Cyclic Mo ... not specified relA
Drugbank DB04315	Guanosine-5'-Diphosphate not specified relA

Comment	Deletion of p65 (RelA) results in differential expression of genes involved in HSC fate. [Method: HSC (CD135-KSL) from p65(hem-/-) mice, qRT-PCR]
Formal Description Interaction-ID: 41974	gene/protein Rela decreases_expression of gene/protein Ly86 in HSC (CD135-KSL)
Drugbank entries	Show/Hide entries for Rela
Drugbank DB02836	Guanosine 5'-Diphosphate 2':3'-Cyclic Mo ... not specified relA
Drugbank DB04315	Guanosine-5'-Diphosphate not specified relA
Drugbank DB02836	Guanosine 5'-Diphosphate 2':3'-Cyclic Mo ... not specified relA
Drugbank DB04315	Guanosine-5'-Diphosphate not specified relA

Comment	CD135-KSL cells from p65(hem-/-) mice show a decreased differentiation potential in a cytokine-rich environment in vitro. [Method: HSC (CD135-KSL) from p65(hem-/-) mice, cultured in methylcellulose media with cytokines for 7 days, average number of cells]
Formal Description Interaction-ID: 41981	gene/protein Rela increases_activity of process hematopoietic stem cell differentiation of HSC (CD135-KSL) in vitro
Drugbank entries	Show/Hide entries for Rela
Drugbank DB02836	Guanosine 5'-Diphosphate 2':3'-Cyclic Mo ... not specified relA
Drugbank DB04315	Guanosine-5'-Diphosphate not specified relA
Drugbank DB02836	Guanosine 5'-Diphosphate 2':3'-Cyclic Mo ... not specified relA
Drugbank DB04315	Guanosine-5'-Diphosphate not specified relA

Comment	Proliferation of p65-deficient HSPCs was increased. Number of dividing KSL cells increases by threefold] [Method: KSL cells from p65(hem-/-) mice, injected with EdU 18 hours prior to harvest]
Formal Description Interaction-ID: 41984	gene/protein Rela decreases_activity of process cell cycle of KSL cells in vivo
Drugbank entries	Show/Hide entries for Rela
Drugbank DB02836	Guanosine 5'-Diphosphate 2':3'-Cyclic Mo ... not specified relA
Drugbank DB04315	Guanosine-5'-Diphosphate not specified relA
Drugbank DB02836	Guanosine 5'-Diphosphate 2':3'-Cyclic Mo ... not specified relA
Drugbank DB04315	Guanosine-5'-Diphosphate not specified relA

Comment	Self-renewal activity is impaired in p65(hem-/-) mice. [Method: competitive transplantation: BM cells from p65(hem-/-) mice transplanted into recipients (PepBoy, 6-8 wks-old), 20 wks posttransplant, secondary transplantation]
Formal Description Interaction-ID: 41985	gene/protein Rela increases_activity of process self-renewal in vivo
Drugbank entries	Show/Hide entries for Rela
Drugbank DB02836	Guanosine 5'-Diphosphate 2':3'-Cyclic Mo ... not specified relA
Drugbank DB04315	Guanosine-5'-Diphosphate not specified relA
Drugbank DB02836	Guanosine 5'-Diphosphate 2':3'-Cyclic Mo ... not specified relA
Drugbank DB04315	Guanosine-5'-Diphosphate not specified relA

Comment	Self-renewal activity is impaired in p65(hem-/-) mice. [Method: competitive transplantation: BM cells from p65(hem-/-) mice transplanted into recipients (PepBoy, 6-8 wks-old), 20 wks posttransplant, secondary transplantation]
Formal Description Interaction-ID: 41986	process I-kappaB kinase/NF-kappaB signaling increases_activity of process self-renewal in vivo

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