Clementi et al. (2005) reported 4 patients with non-Hodgkin lymphoma with features of hemophagocytic lymphohistiocytosis (603533) who had mutations in the perforin gene (see 170280.0002; 170280.0009; 170280.0011).
- Somatic Mutations ... - Germline Mutations Clementi et al. (2005) reported 4 patients with non-Hodgkin lymphoma with features of hemophagocytic lymphohistiocytosis (603533) who had mutations in the perforin gene (see 170280.0002; 170280.0009; 170280.0011). - Somatic Mutations DLBCL is the most common form of non-Hodgkin lymphoma, accounting for 30 to 40% of cases (Lenz et al., 2008). DLBCL consists of 3 subtypes: germinal center B cell-like (GCB) DLBCL, ABC DLBCL, and primary mediastinal B cell lymphoma. The ABC DLBCL subtype accounts for roughly one-third of the cases and has an inferior prognosis. A characteristic feature of ABC DLBCL is constitutive activation of the NF-kappa-B (see 164011) pathway, which plays a pivotal role in proliferation, differentiation, and survival of normal lymphoid cells. In normal B cells, antigen receptor-induced NF-kappa-B activation requires CARD11 (607210), a cytoplasmic scaffolding protein. To determine whether CARD11 contributes to tumorigenesis, Lenz et al. (2008) sequenced the CARD11 gene in human DLBCL tumors. Lenz et al. (2008) detected missense mutations in 7 of 73 ABC DLBCL biopsies (9.6%), all within exons encoding the coiled-coil domain. Experimental introduction of CARD11 coiled-coil domain mutants into lymphoma cell lines resulted in constitutive NF-kappa-B activation and enhanced the NF-kappa-B activity upon antigen receptor stimulation. Lenz et al. (2008) concluded that CARD11 is a bona fide oncogene in DLBCL. Compagno et al. (2009) showed that greater than 50% of ABC DLBCL and a smaller fraction of germinal center B cell-like (GCB) DLBCL carry somatic mutations in multiple genes, including negative (TNFAIP3; 191163) and positive (including CARD11 and TRAF2, 601895) regulators of NF-kappa-B. Of these, the TNFAIP3 gene, which encodes a ubiquitin-modifying enzyme (A20) involved in termination of NF-kappa-B responses, is most commonly affected, with approximately 30% of patients displaying biallelic inactivation by mutations and/or deletions. When reintroduced in cell lines carrying biallelic inactivation of the gene, A20 induced apoptosis and cell growth arrest, indicating a tumor suppressor role. Less frequently, missense mutations of TRAF2 and CARD11 produce molecules with significantly enhanced ability to activate NF-kappa-B. Compagno et al. (2009) concluded that NF-kappa-B activation in DLBCL is caused by genetic lesions affecting multiple genes, the loss or activation of which may promote lymphomagenesis by leading to abnormally prolonged NF-kappa-B responses. Morin et al. (2010) identified recurrent somatic mutations affecting the tyr641 residue of the conserved SET domain in the EZH2 gene (601573) in cases of follicular lymphoma and diffuse large B-cell lymphoma of only the GCB subtype. Their data indicated that mutation involving this tyrosine was among the most frequent genetic events observed in GCB malignancies, after t(14;18)(q32;q21) translocations. Pasqualucci et al. (2011) reported that the 2 most common types of B cell non-Hodgkin lymphoma, follicular lymphoma and diffuse large B-cell lymphoma, harbor frequent structural alterations inactivating CREBBP (600140) and, more rarely, EP300 (602700), 2 highly related histone and nonhistone acetyltransferases (HATs) that act as transcriptional coactivators in multiple signaling pathways. Overall, about 39% of diffuse large B-cell lymphoma and 41% of follicular lymphoma cases display genomic deletions and/or somatic mutations that remove or inactivate the HAT coding domain of these 2 genes. These lesions usually affect 1 allele, suggesting that reduction in HAT dosage is important for lymphomagenesis. Pasqualucci et al. (2011) demonstrated specific defects in acetylation-mediated inactivation of the BCL6 oncoprotein (109565) and activation of the p53 tumor suppressor (191170). Morin et al. (2011) sequenced tumor and matched control DNA from 13 DLBCL cases and 1 follicular lymphoma case to identify genes with mutations in B-cell NHL. Morin et al. (2011) analyzed RNA sequencing data from these and another 113 NHLs to identify genes with candidate mutations, and then resequenced tumor and matched normal DNA from these cases to confirm 109 genes with multiple somatic mutations. Genes with roles in histone modification were frequent targets of somatic mutation. For example, 32% of DLBCL and 89% of follicular lymphoma cases had somatic mutations in MLL2 (602113), which encodes a histone methyltransferase, and 11.4% and 13.4% of DLBCL and follicular lymphoma cases, respectively, had mutations in MEF2B (601661), a calcium-regulated gene that cooperates with CREBBP and EP300 in acetylating histones. Morin et al. (2011) concluded that their analysis suggested a previously unappreciated disruption of chromatin biology in lymphomagenesis. In their analysis, Morin et al. (2011) found that the 8 most significant genes included 7 with strong selective pressure for nonsense mutations, including the known tumor suppressor genes TP53 and TNFRSF14 (602746). CREBBP also showed some evidence for acquisition of nonsense mutations and coding single-nucleotide variants. Morin et al. (2011) observed enrichment for nonsense mutations in BCL10 (603517), a positive regulator of NF-kappa-B in which oncogenic truncated products have been described in lymphomas. The remaining strongly significant genes (BTG1, 109580; GNA13, 604406; SGK1, 602958; and MLL2, 602113) had no reported role in lymphoma. GNA13 encodes the alpha subunit of a heterotrimeric G protein-coupled receptor responsible for modulating RhoA activity. SGK1 encodes a phosphatidylinositol-3-hydroxy kinase-regulated kinase with functions including regulation of FOXO transcription factors, regulation of NF-kappa-B by phosphorylating I-kappa-B kinase (see 600664), and negative regulation of NOTCH (190198) signaling. SGK1 also resides within a region of chromosome 6 commonly deleted in DLBCL. Both SGK1 and GNA13 mutations were found only in germinal center B-cell lymphomas. MEF2B and TNFRSF14, which had no previously described role in DLBCL, were similarly restricted to germinal center B-cell lymphomas. To identify tumor suppressor genes in lymphoma, Scuoppo et al. (2012) screened a short hairpin RNA library targeting genes deleted in human lymphomas and functionally confirmed those in a mouse lymphoma model. Of the 9 tumor suppressors identified, 8 corresponded to genes occurring in 3 physically linked 'clusters,' suggesting that the common occurrence of large chromosomal deletions in human tumors reflects selective pressure to attenuate multiple genes. Among the new tumor suppressors are adenosylmethionine decarboxylase-1 (AMD1; 180980) and eukaryotic translation initiation factor 5A (eIF5A; 600187), 2 genes associated with hypusine, a unique amino acid produced as a product of polyamine metabolism through a highly conserved pathway. Through a secondary screen surveying the impact of all polyamine enzymes on tumorigenesis, Scuoppo et al. (2012) established the polyamine-hypusine axis as a new tumor suppressor network regulating apoptosis. Unexpectedly, heterozygous deletions encompassing AMD1 and eIF5A often occur together in human lymphomas, and cosuppression of both genes promotes lymphomagenesis in mice. Thus, Scuoppo et al. (2012) concluded that some tumor suppressor functions can be disabled through a 2-step process targeting different genes acting in the same pathway.