The MIPS Mammalian Protein-Protein Interaction Database

The MIPS Mammalian Protein-Protein Interaction Database is a collection of manually curated high-quality PPI data collected from the scientific literature by expert curators. We took great care to include only data from individually performed experiments since they usually provide the most reliable evidence for physical interactions.

Search the database

To suit different users needs we provide a variety of interfaces to search the database:


Protein-protein interactions (PPI) represent a pivotal aspect of protein function. Almost every cellular process relies on transient or permanent physical binding of two or more proteins in order to accomplish the respective task. Comprehensive databases of PPI in Saccharomyces cerevisiae have proved to be invaluable resources for both bioinformatics and experimental research and are used heavily in the scientific community.

Although yeast is a well established model organism, not all interactions in higher eukaryotes have equivalent counterparts in unicellular systems. Currently, publicly available PPI databases contain comparatively few entries from mammals so we embarked on building a high-quality, manually curated database of protein-protein interactions in mammals.

Conditions of use

You are free to use the database as you please including full download of the dataset for your own analyses as long as you cite the source properly:

Pagel P, Kovac S, Oesterheld M, Brauner B, Dunger-Kaltenbach I, Frishman G, Montrone C, Mark P, Stümpflen V, Mewes HW, Ruepp A, Frishman D
The MIPS mammalian protein-protein interaction database
Bioinformatics 2005; 21(6):832-834; [Epub 2004 Nov 5]   doi:10.1093/bioinformatics/bti115   PubMed

Other PPI resources

There are plenty of interesting databases and other sites on protein-protein interactions. Currently we are aware of the following PPI resources:

Resource Comments
APID Agile Protein Interaction DataAnalyzer (Cancer Research Center, Salamanca, Spain)
BIND Biomolecular INteraction Network Database at the University of Toronto, Canada. No species restriction
CYGD PPI section of the Comprehensive Yeast Genome Database. Manually curated comprehensive S. cerevisiae PPI database at MIPS
DIP Database of Interacting Proteins at UCLA. No species restriction.
GRID General Repository for Interaction Datasets. Mount Sinai Hospital, Toronto, Canada
HIV Interaction DB Interactions between HIV and host proteins.
HPRD The Human Protein Reference Database. Institute of Bioinformatics, Bangalore, India and Johns Hopkins University, Baltimore, MD, USA.
HPID Human Protein Interaction Database. Department of computer Science and Information Engineering Inha University, Inchon, Korea
iHOP iHOP (Information Hyperlinked over Proteins). Protein association network built by literature mining
IntAct Protein interaction database at EBI. No species restriction.
InterDom Database of putative interacting protein domains. Institute for InfoComm Research, Singapore.
JCB PPI site at the Jena Centre for Bioinformatics, Germany
MetaCore Commercial software suite and database. Manually curated human PPIs (among other things). GeneGo
MINT Molecular INTeraction database at the Centro di Bioinformatica Moleculare, Universita di Roma, Italy.
MRC PPI links Commented list of links to PPI databases and resources maintained at the MRC Rosalind Franklin Cetre for Genomics Research, Cambridge, UK
OPHID The Online Predicted Human Interaction Database. Ontario Cancer Institute and University of Toronto, Canada.
Pawson Lab Information on protein-interaction domains.
PDZbase Database of PDZ mediated protein-protein interactions.
Predictome Predicted functional associations and interactions. Boston University.
Protein-Protein Interaction Server Analysis of protein-protein interfaces of protein complexes from PDB. University College of London, UK.
PathCalling Proteomics and PPI tool/database. CuraGen Corporation.
PIM Hybrigenics PPI data and tool, H. pylori. Free academic license available
RIKEN Experimental and literature PPIs in mouse.
STRING Protein networks based on experimental data and predictions at EMBL.
YPD "BioKnowledge Library" at Incyte Corporation. Manually curated PPI data from S. cerevisiae. Proprietary.

If we forgot to list your favorite PPI resource or you are providing one yourself please let us know - we will be happy to include it.

PPI related software

Resource Comments
aiSee Commercial graph layout software
Cytoscape Open source software for visualization of PPI networks and data integration
graphviz Graph layout software


You can get the full dataset here (PSI-MI format).


This work is funded by a grant from the German Federal Ministry of Education and Research. It is part of the initiative "Bioinformatics for the Functional Analysis of Mammalian Genomes" (BFAM).


For questions, criticism, praise and suggestions please contact Dr. Philipp Pagel or Dr. Dmitrij Frishman.