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General information


186
18246122
Homo sapiens (9606)
disease
Lung cancer

Study information


Cell culture study
Calu-1 cell
In addition, the following NSCLC (non-small cell lung cancer) cell lines were analyzed: A459, A549, H460. Goal of this paper was to differentiate the MicroRNA signature between TRAIL-(TNF-related apoptosis-inducing ligand) resistant and TRAIL-sensitive cells. Calu-1 = TRAIL-resistant, A459, A549 = semi-resistant, H460 = sensitive to TRAIL.

miRNA list


Nr miRNA Method Further methods Evidence Foldchange Genes
1 hsa-mir-100 miRNA microarray quantitative PCR miRNA overexpression 8.3
2 hsa-mir-125b-1 miRNA microarray quantitative PCR miRNA overexpression 5.4
3 hsa-mir-15b miRNA microarray miRNA overexpression 3.6
4 hsa-mir-221 miRNA microarray quantitative PCR miRNA overexpression 5.9 CDKN1BKIT
5 hsa-mir-222 miRNA microarray quantitative PCR, Northern Blot miRNA overexpression 8.6 CDKN1BKIT
6 hsa-mir-9-1 miRNA microarray quantitative PCR miRNA downregulation 13.9
7 hsa-mir-96 miRNA microarray miRNA downregulation 2

Detailed information about selected miRNA


hsa-mir-100
miRNA microarray
quantitative PCR
miRNA overexpression
8.3    

Genes associated with selected miRNA


target gene regulation mechanism of regulation comment

Comments


General comment: The inhibition of miR-221 and -222 expression with the specific anti-miR inhibitor in CALU-1 TRAIL-resistant cells was able to change the insensitive TRAIL phenotype to a sensitive one. Forced overexpression of the miR-222, -100 and -221, but not of miR-125b, in the sensitive H460 cells increased resistance to TRAIL in these cells.