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About CYGD


MIPS Fungal Genomes

mips home MIPS-FUNGI-ADM      FTP
CHS3 Chitin synthase III 
Entry:YBR023c
Alias:CAL5; CSD2; DIT101; KTI2; YBR0305; (CAL1)
Classification:known protein | 5422 Entries | Evi | PUBMED
Feature Type:CDS
Features
   Blast-Simap    DNA VIEW    PROTEIN VIEW

Similarity:
Paralogs (11.2 %) ;  |  Homologs in   Hemiascomycota (97.3 %);  Basidiomycota (46.5 %);  Fungi (97.3 %);  Eukaryota (97.3 %);  Plants (6.2 %);  Mammalia (9.1 %);  Human (9 %);  Bacteria (8.6 %);  All except yeast (97.3 %)

    Functional Classification:
    • CELL RESCUE, DEFENSE AND VIRULENCE
      ..stress response
      ....osmotic and salt stress response | 59 Entries | Evi | PUBMED
    • CELL TYPE DIFFERENTIATION
      ..fungal/microorganismic cell type differentiation
      ....fungal and other eukaryotic cell type differentiation
      ......development of asco- basidio- or zygospore | 167 Entries | Evi
    • METABOLISM
      ..C-compound and carbohydrate metabolism
      ....polysaccharide metabolism
      ......chitin metabolism
      ........chitin anabolism | 8 Entries | Evi | PUBMED
    • CELL TYPE DIFFERENTIATION
      ..fungal/microorganismic cell type differentiation
      ....fungal and other eukaryotic cell type differentiation
      ......budding, cell polarity and filament formation | 313 Entries | Evi
    • INTERACTION WITH THE ENVIRONMENT
      ..cellular sensing and response to external stimulus
      ....chemoperception and response
      ......pheromone response, mating-type determination, sex-specific proteins | 189 Entries | Evi
    • CELL CYCLE AND DNA PROCESSING
      ..cell cycle
      ....cytokinesis (cell division) /septum formation and hydrolysis | 71 Entries | Evi | PUBMED
    EC Number:
    • 2.4.1.16: Transferases
      ..Glycosyltransferases
      ....Hexosyltransferases
      ......chitin synthase | 3 Entries | Evi | PUBMED
    Pathway:
    • chitin biosynthesis | chitin biosynthesis
    TC Class:
    • Non Transporting Mebrane Proteins
      ..Polysaccharides
      ....Wall
      ......The Chitin Synthesis Subfamily | 3 Entries | Evi | PUBMED
    InterPro:
    Localization:  CHS3 localization details
    • bud
      ..neck
    • plasma membrane
    • golgi
    • transport vesicles
      ..golgi-vacuole transport vesicles
    Remarks:
    • transported to the plasma membrane via the secretory pathway, see | PUBMED
    • Chs3p and Chs1p are continously synthesized and remain metabolically stable, see | PUBMED
    • regulation can occur by activation of latent forms
    • distribution and amount is not cell cycle regulated
    • Chs1p and Chs3p reside partly in chitosomes
    • chitosome production is blocked in end4-1 endocytosis mutant
    • endocytosis is required for formation or maintenance of chitosomes
    • colocalizes with Chs1p and Ste2p to chitosomes
    • in contrast to Ste2p, Chs1p and Chs3p are not rapidly degraded
    • In the absence of CHS7 product, Chs3p, but not other secreted proteins, is retained in the ER, leading to a severe defect in CSIII activity and consequently, to a reduced rate of chitin synthesis | PUBMED
    • C-terminal region must be in cytoplasm for activity
    • found as a zymogen that can be activated in vitro by partial proteolysis
    Phenotypes:  Disruption: CHS3 details
    • viable
    ___
    • null mutant has 10-fold reduction in chitin content
    • mutants are resistance to Calcofluor and have very little chitin
    • mutant cells are resistant to K. lactis killer toxin
    • mutants lack chitosan in spore walls
    • chs3/gas1 double null mutants show severe reduction in growth rate and cell viability
    • chs3/gas1 double null mutants show the loss of the typical structure of cell walls
    ___
    • Cell morphology and organelle mutants
      ..Cell wall mutants
      ....Calcofluor white
      ......Calcofluor white resistance | 19 Entries
    • Cell morphology and organelle mutants
      ..Cell wall mutants
      ....Killer toxin
      ......Killer toxin resistance | 21 Entries
    • Cell morphology and organelle mutants
      ..Spore wall mutants | 7 Entries
    Click for larger Map
    Physical Features
    Coordinates:287925 - 284428 (C)
    Length:3498 nt
    Contig:chrII
    Exon Coordinates:287925 - 284428
    GC Content:39.2 %
    Protein Length:1165 aa
    Isoelectric Point:6.26
    Molecular Weight:131602 Da
    Transmembrane Domains:Begin - End / Length (aa)
    168 - 190 / 23
    203 - 224 / 22
    454 - 476 / 23
    1029 - 1051 / 23
    1055 - 1077 / 23
    1084 - 1106 / 23

    6 TM- Domains
    Retrieve Sequence: DNA /Protein
    Literature | References
    CYGD curated Lit.:PubMed
    PubMed:Search abstracts
    PubMed Central:Search full-text articles
    Sequence References:
    EMBL: Z35892
    PIR: S45879
    UniProtKB: P29465
    NCBI: NP_009579
    Additional Links:
    LinkOut:
  • SGD - Saccharomyces Genome Database
  • YGOB - Yeast Gene Order Browser
  • yMGV - yeast Microarray Global Viewer

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