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MPact protein interaction

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About CYGD

MIPS Fungal Genomes

mips home MIPS-FUNGI-ADM      FTP
GAL4 Transcription factor 
Alias:DGR2; GAL81; P1021
Classification:known protein | 5422 Entries | Evi | PUBMED
Feature Type:CDS
   Blast-Simap    DNA VIEW    PROTEIN VIEW

Paralogs (18.5 %) ;  |  Homologs in   Hemiascomycota (84.2 %);  Basidiomycota (20.6 %);  Fungi (84.2 %);  Eukaryota (100 %);  Plants (16.4 %);  Mammalia (11.1 %);  Human (8 %);  Bacteria (13.4 %);  All except yeast (100 %)

  • similarity to K.lactis Lac9 and S.pombe thi1 protein
  • structurally and functionally related to lac9 of K.lactis
  • member of the binuclear zinc cluster proteins
Functional Classification:
    ..C-compound and carbohydrate metabolism
    ....regulation of C-compound and carbohydrate metabolism | 127 Entries | Evi
    ..RNA synthesis
    ....mRNA synthesis
    ......transcriptional control
    ........transcription activation | 42 Entries | Evi | PUBMED
Localization:  GAL4 localization details
  • nucleus
  • remains bound to Gal80p during activation of Gal4p
  • active state may be fortuitously phosphorylated since mutation of the phosphorylation site does not affect activation
  • activation target is probably a protein or proteins in the basal initiation complex
  • both phosphorylated and unphosphorylated Gal4p bind equally well to DNA and to Gal80p in vitro
  • Gal4p is produced in all carbon sources and expression is not induced by galactose
  • Egd1p helps Gal4p binding to DNA
  • phosphorylation correlates with activation activity
  • Kd of Gal4p-Gal80p interaction is about 5 x 10E-9 M
  • contains three domains mediating a direct glucose inhibition of Gal4p
  • transcription activation and Gal80p binding share the same region, but are mutually separable
  • alterated GAL4 DNA-binding domain can affect transcriptional activation independent of DNA binding | PUBMED
  • contains a Zn[2]-Cys[6] fungal-type binuclear cluster domain in the N-terminal region
  • two phosphorylated forms, Gal4p-II and Gal4p-III
  • residues 14-57 contains the C6 zinc cluster which binds specifically to CGG triplets at the UAS-GAL termini
  • Kd for binding to the GAL1-10 promoter containing all four UAS-GAL elements is 2 x 10E-9 M
Phenotypes:  Disruption: GAL4 details
  • viable
  • Exhibits sensitivity at 15 generations when grown in galactose. | Evi | PUBMED
  • Exhibits sensitivity at 5 generations when grown in galactose. | Evi | PUBMED
  • overexpression causes altered distribution of cell wall chitin
  • SUG2 was identified as a suppressor of gal4 phenotpye | PUBMED
Click for larger Map
Physical Features
Coordinates:82356 - 79711 (C)
Length:2646 nt
Exon Coordinates:82356 - 79711
GC Content:40.8 %
Protein Length:881 aa
Isoelectric Point:6.79
Molecular Weight:99403.3 Da
Retrieve Sequence: DNA /Protein
Literature | References
CYGD curated Lit.:PubMed
PubMed:Search abstracts
PubMed Central:Search full-text articles
Sequence References:
EMBL: Z73604
EMBL: K01486
PIR: A05022
UniProtKB: P04386
NCBI: NP_015076
Additional Links:
CYGD Pathways:
  • The role of MIG1 in metabolic functions
  • CYGD Tables:
  • Impact of Mig1p upon the expression of other genes
  • LinkOut:
  • SGD - Saccharomyces Genome Database
  • YGOB - Yeast Gene Order Browser
  • yMGV - yeast Microarray Global Viewer

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