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| Search ORF/Gene/Description |
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Chromosome
(chrI - chrXVI,chrMito,2-micron) |
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| Entry: | YOL090w | | Alias: | O0935 | | Classification: | known protein | 5422 Entries | Evi
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PUBMED
| | Feature Type: | CDS | | Features |  |
Blast-Simap
DNA VIEW
PROTEIN VIEW
| | Similarity: |
similarity to human MSH2 and other DNA mismatch repair proteinssimilarity to human hereditary non-polyposis colon cancer protein MSH2 | | Functional Classification: | - CELL RESCUE, DEFENSE AND VIRULENCE
..stress response
....DNA damage response
| 77 Entries | Evi
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PUBMED
- CELL CYCLE AND DNA PROCESSING
..DNA processing
....DNA recombination and DNA repair
......DNA repair
| 159 Entries | Evi
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PUBMED
- CELL CYCLE AND DNA PROCESSING
..DNA processing
....DNA recombination and DNA repair
......DNA recombination
........somatic / mitotic recombination
| 21 Entries | Evi
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PUBMED
- PROTEIN WITH BINDING FUNCTION OR COFACTOR REQUIREMENT (structural or catalytic)
..nucleotide/nucleoside/nucleobase binding
....ATP binding
| 191 Entries | Evi
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PUBMED
- CELL CYCLE AND DNA PROCESSING
..cell cycle
....mitotic cell cycle and cell cycle control
......cell cycle checkpoints (checkpoints of morphogenesis, DNA-damage,-replication, mitotic phase and spindle)
| 67 Entries | Evi
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PUBMED
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Pathway: | mismatch repair
| postmismatch recognition - responsible for inhibition of recombination when mismatches are still present |
PUBMED
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InterPro:
| | | Localization: | MSH2 localization details | | Remarks: | instability of trinucleotide repeats is not dependent on Msh2p, Msh3p, Pms1p or Rad52p function |
PUBMED
Msh2p-Holliday junctions are more stable than Msh2p-duplex DNA complexesstabilizing effect by variant repeats in microsatellite sequences is independent of pms1 msh2 and msh3 |
PUBMED
a single mismatch is sufficient for inhibition of recombination, additional mismatches have a cumulative negative effect on recombination rate |
PUBMED
when the flanking homologous regions that anneal are 205 bp, the requirement for Msh2/Msh3 is as great as for Rad1/Rad10; but when the annealing partners are 1,170 bp, Msh2/Msh3 have little effect, while Rad1/Rad10 are still required |
PUBMED
the carboxy-terminal 114 aa are important for interaction with Msh6p | | Phenotypes: | Disruption: MSH2 details___
mutants have greatly increased mutation rate to canavanine resistancemutants are defective in repair of single base mismatchesmutants have high rate of 2- to 4- base pair insertions or deletions at dinucleotide repeatsmutant have 50-fold elevated spontaneous mutation ratenull mutant loses polarity of gene conversion usually found across the length of a geneincreased 1Bp deletions, preferential at homonucleotide runsdramatically increased mutation rate in homonucleotide runs(1Bp deletions)synergistic increase in +1 and -1 frameshifts in msh2 pol2-4 mutants (pol2 proofreading mutation)destabilizes microsatellites with repeat units of 1 to 13 bp but has no effect on microsatellites 16 to 20 bp repeatesexo1 msh2 and rad2 msh2 double mutants are more sensitive to UV than the single mutations |
PUBMED
msh2 deletion mutant shows a 290-fold increase in GT-tract instability |
PUBMED
P640L conversion results in a complete loss of function in vivo |
PUBMED
H658Y conversion retains full function of the protein in vivo |
PUBMED
___
Nucleic acid metabolism defects
..DNA repair mutants
....other DNA repair mutants | 32 Entries |
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