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MIPS Fungal Genomes

mips home MIPS-FUNGI-ADM      FTP
YKU70 High-affinity DNA-binding protein 
Entry:YMR284w
Alias:HDF1; KU70; NES24; OBF2; YM8021.10
Classification:known protein | 5422 Entries | Evi | PUBMED
Feature Type:CDS
Features
   Blast-Simap    DNA VIEW    PROTEIN VIEW

Similarity:
Paralogs (18.9 %) ;  |  Homologs in   Hemiascomycota (90.7 %);  Basidiomycota (21.3 %);  Fungi (90.7 %);  Eukaryota (90.7 %);  Plants (21.1 %);  Mammalia (21.9 %);  Human (20.4 %);  Bacteria (19 %);  All except yeast (90.7 %)

  • weak similarity to human 70K thyroid autoantigen
  • has 22% identity over 298 amino acids to human 67 kDa subunit of human Ku autoantigen
  • related to human ku autoantigen p70 chain
  • human homolog is probably the XRCC6 gene product
Functional Classification:
  • CELL CYCLE AND DNA PROCESSING
    ..DNA processing
    ....DNA synthesis and replication | 138 Entries | Evi
  • CELL CYCLE AND DNA PROCESSING
    ..DNA processing
    ....DNA recombination and DNA repair
    ......DNA repair | 159 Entries | Evi | PUBMED
  • PROTEIN WITH BINDING FUNCTION OR COFACTOR REQUIREMENT (structural or catalytic)
    ..nucleic acid binding
    ....RNA binding | 190 Entries | Evi | PUBMED
  • CELL CYCLE AND DNA PROCESSING
    ..DNA processing
    ....DNA restriction or modification
    ......DNA conformation modification (e.g. chromatin) | 187 Entries | Evi | PUBMED
  • CELL CYCLE AND DNA PROCESSING
    ..cell cycle
    ....mitotic cell cycle and cell cycle control
    ......cell cycle checkpoints (checkpoints of morphogenesis, DNA-damage,-replication, mitotic phase and spindle) | 67 Entries | Evi | PUBMED
Pathway:
  • | component of DNA end-joining repair pathway
InterPro:
Localization:  YKU70 localization details
  • cytoplasm
  • nucleus
Remarks:
  • may be a subunit of DNA-dependent protein kinase
  • in the absence of YKU70p, residual plasmid repair operates through an error-prone pathway that involves the deletion of terminal DNA sequences
    Phenotypes:  Disruption: YKU70 details
    • viable
    ___
    • mutants have weak sensitivity to neomycin at high temperature
    • mutants have growth defect at 37C | PUBMED
    • ts mutant arrests at non-permissive temperature in G2
    • null mutant does not affect UV or gamma-ray sensitivity
    • in rad52 background, which has no homologous recombination, null mutant has additional sensitivity to methylmethanesulfonate (MMS) and UV-irradiation
    • hdf1 mutant strains have substantially shorter telomeres than wild-type strains
    • hdf1 tel1 double mutant have extremely short telomeres and grow slowly
    • yku70 mutants do not show increased sensitivity towards low or high doses of IR, hydroxyurea, UV and MMS
    • in yku70 mutants terminal deletions of up to several hundred bp occur before ligation ensues
    • null mutant form inviable colonies (replica does not grow) - in liquid culture null mutant viability decrease after several generation times
    • in null mutant culture half of the cells show large-budded cells with a short spindle (G2/M arrest) and the other half are unbudded cells (G1 arrest) at 37 deg
    • hdf1 null mutant large budded cells shows only one nucleus at 37 deg
    • hdf1 rad9 double mutant show divided and segregated nuclei in the large-budded cells at 37 deg
    • hdf1 null mutants shows increased transcription rate of RNR2 and RNR3 at 20 deg and 37 deg
    • in hdf1 null mutants most of the large budded cells have a 4N DNA content and most of the unbudded cells have 2N DNA content at 37 deg
    • hdf1 cell cycle arrest is Rad9p dependent
    • in sir2,sir3,sir4 and hdf1 mutants circularization of non-homologous DNA-ends is reduced about 20-fold | PUBMED
    • mutants show increased single-strandedness of the telomere in a cell-cycle-independent fashion, unlike wild-type cells which form 3^ poly(TG1-3) overhangs exclusively in late S phase | PUBMED
    • mutations enhance the instability of elongated telomeres to degradation and recombination | PUBMED
    • Exhibits sensitivity at 5 generations when grown in 10 uM nystatin. | Evi | PUBMED
    • Exhibits sensitivity at 5 generations when grown in synthetic complete medium. | Evi | PUBMED
    ___
    • Conditional phenotypes
      ..Heat-sensitivity (ts) | 313 Entries
    Suppression:
    • Drosophila and human Ku p70 complement the hfd1 null mutant phenotype
    Click for larger Map
    Physical Features
    Coordinates:838186 - 839994 (W)
    Length:1809 nt
    Contig:chrXIII
    Exon Coordinates:838186 - 839994
    GC Content:33.8 %
    Protein Length:602 aa
    Isoelectric Point:6.01
    Molecular Weight:70647.7 Da
    Retrieve Sequence: DNA /Protein
    Literature | References
    CYGD curated Lit.:PubMed
    PubMed:Search abstracts
    PubMed Central:Search full-text articles
    Sequence References:
    EMBL: D15052
    EMBL: Z49704
    PIR: S54591
    UniProtKB: P32807
    NCBI: NP_014011
    Additional Links:
    CYGD Pathways:
  • The multiple roles of Mre11 complex in repair and recombination
  • LinkOut:
  • SGD - Saccharomyces Genome Database
  • YGOB - Yeast Gene Order Browser
  • yMGV - yeast Microarray Global Viewer

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