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MPact protein interaction

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About CYGD

MIPS Fungal Genomes

mips home MIPS-FUNGI-ADM      FTP
YEF3 Translation elongation factor eEF3 
Alias:L9672.5; YEF3A; (EFC1); (TEF3)
Classification:known protein | 5422 Entries | Evi | PUBMED
Feature Type:CDS
   Blast-Simap    DNA VIEW    PROTEIN VIEW

Paralogs (83.8 %) ;  |  Homologs in   Hemiascomycota (98.8 %);  Basidiomycota (48.4 %);  Fungi (98.8 %);  Eukaryota (98.8 %);  Plants (45.8 %);  Mammalia (16.4 %);  Human (16.3 %);  Bacteria (18.3 %);  All except yeast (98.8 %)

  • strong similarity to YNL014w, YPL226w and C.albicans translation elongation factor eEF-3
  • belongs to the ATP-binding transport protein family (ABC transporters) EF-3 subfamily
  • has some similarity to bacterial membrane transporters
Functional Classification:
  • translation | elongation of peptide synthesis
Localization:  YEF3 localization details
  • cytoplasm
  • found only in fungi
  • phosphorylated
  • has ribosome-dependent ATPase and GTPase activity
  • aa 819-857 shows similarity to aminoacyl-tRNA synthetases | PUBMED
  • a C-terminal 30k fragment (AA775-1044) associates stringently with ribosomes
  • a N-terminal 90k fragment (AA1-774) shows ribosome-independent ATPase activity
  • C-terminus contains blocks of lysine boxes essential for functional interaction with ribosome | PUBMED
  • S5-homologous domain (Lys102-Ile368) | PUBMED
  • N-terminal ATP-binding cassette (Gly463-Lys622) | PUBMED
  • aminoacyl-tRNA-synthase homologous domain (Glu820-Gly865) | PUBMED
Phenotypes:  Disruption: YEF3 details
  • lethal
  • mutants are hypersensitive to calcofluor white | PUBMED
  • mutants are hypersensitive to zymolyase | PUBMED
  • mutants show increased level of N-acetylglucosamine (GlcNac) relative to glucose and mannose in cell wall | PUBMED
  • Cell morphology and organelle mutants
    ..Cell wall mutants
    ....Calcofluor white
    ......Calcofluor white sensitivity | 88 Entries
  • Cell morphology and organelle mutants
    ..Cell wall mutants
    ....other cell wall mutants | 73 Entries
  • overexpressors are more sensitive to hygromycin and paromomycin, which cause a decrease in translational fidelity
  • ovrexpression results in slow growth phenotype | PUBMED
Click for larger Map
Physical Features
Coordinates:636782 - 639916 (W)
Length:3135 nt
Exon Coordinates:636782 - 639916
GC Content:41.8 %
Protein Length:1044 aa
Isoelectric Point:5.73
Molecular Weight:115945 Da
Retrieve Sequence: DNA /Protein
Literature | References
CYGD curated Lit.:PubMed
PubMed:Search abstracts
PubMed Central:Search full-text articles
Sequence References:
EMBL: U20865
PIR: S59395
NCBI: NP_013350
Additional Links:
  • SGD - Saccharomyces Genome Database
  • YGOB - Yeast Gene Order Browser
  • yMGV - yeast Microarray Global Viewer

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