| Entry: | YLR127c |
| Alias: | L3108; L9233.13; RSI1; TID2 |
| Classification: | known protein | 5422 Entries | Evi
|
PUBMED
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| Feature Type: | CDS |
| Overlapping Feature: | |
| Features |
 |
Blast-Simap
DNA VIEW
PROTEIN VIEW
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| Similarity: |
similarity to human Apc2p |
PUBMED
weak similarity to fruit fly lin19 protein |
| Functional Classification: | - PROTEIN WITH BINDING FUNCTION OR COFACTOR REQUIREMENT (structural or catalytic)
..nucleotide/nucleoside/nucleobase binding
....ATP binding
| 191 Entries | Evi
|
PUBMED
- CELL CYCLE AND DNA PROCESSING
..cell cycle
....mitotic cell cycle and cell cycle control
......mitotic cell cycle
........M phase
| 51 Entries | Evi
|
PUBMED
- PROTEIN FATE (folding, modification, destination)
..protein modification
....modification by ubiquitination, deubiquitination
| 79 Entries | Evi
|
PUBMED
- PROTEIN FATE (folding, modification, destination)
..assembly of protein complexes
| 199 Entries | Evi
|
PUBMED
- PROTEIN FATE (folding, modification, destination)
..protein/peptide degradation
....cytoplasmic and nuclear protein degradation
......proteasomal degradation (ubiquitin/proteasomal pathway)
| 128 Entries | Evi
|
PUBMED
- PROTEIN WITH BINDING FUNCTION OR COFACTOR REQUIREMENT (structural or catalytic)
..protein binding
| 392 Entries | Evi
|
PUBMED
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| EC Number: | - 6.3.2.19: Ligases
..Forming Carbon-Nitrogen Bonds
....Acid?Amino-Acid Ligases (Peptide Synthases)
......ubiquitin?protein ligase
| 47 Entries | Evi
|
PUBMED
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|
InterPro:
| |
| Localization: | APC2 localization details |
| Remarks: | C-terminal region shows similarity to putative ORF K06H7.5 from C.elegans and to a mouse protein |
PUBMED
contains a 180aa region with similarity to the cullin family, which includes yeast Cdc53p |
PUBMED
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| Phenotypes: | Disruption: APC2 details___
temperature-sensitive mutants arrest in metaphasetemperature-sensitive mutants apc2-1 and apc2-4 show cell cycle arrest with large budded cells with the nucleus at or near the neck |
PUBMED
temperature-sensitive mutants apc2-1 and apc2-4 show cell cycle arrest at G2-M with short to midlenght spindles |
PUBMED
spores carrying the apc2 deletion arrest as large, budded cells within one to three cell divisions after germination |
PUBMED
temperature sensitive apc2-1 mutants show wildtype timing of DNA replication and formation of mitotic spindles under non permissive conditions |
PUBMED
temperature sensitive apc2-1 mutants are defective in seperation of sister chromatids and spindle elongation and completely blocked in cytokinesis and re-replication under non-permissive conditions |
PUBMED
arrested temperature sensitive apc2-1 mutants contain elevated levels of Pds1p |
PUBMED
apc2-1 mutants are defective in degradation of Pds1p and Clb2p due to defects in ubiquitination of the proteins |
PUBMED
apc2-1 pds1 delta double mutants do seperate sister chromatids |
PUBMED
apc2-1 pds1 delta double mutants show slower spindle elongation compared to wild type cells |
PUBMED
rsi1-8 pds1 delta can overcome the abaphase block of rsi1 mutants, but arrest then in telophase |
PUBMED
___
Conditional phenotypes
..Heat-sensitivity (ts) | 313 Entries Mating and sporulation defects
..Sporulation efficiency | 112 Entries |
