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Chromosome
(chrI - chrXVI,chrMito,2-micron)

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  XIV  XV  XVI  Mito
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About CYGD


MIPS Fungal Genomes
mips home MIPS-FUNGI-ADM      FTP
SAG1 Alpha-agglutinin 
Entry:YJR004c
Alias:AGALPHA1; AGAL1; J1418
Classification:known protein | 5422 Entries
Feature Type:CDS
Features
   Blast-Simap    DNA VIEW    PROTEIN VIEW
Similarity:
Paralogs (10.6 %) ;  |  Homologs in   Hemiascomycota (74.4 %);  Basidiomycota (14 %);  Fungi (74.4 %);  Eukaryota (74.4 %);  Plants (12.6 %);  Mammalia (14.6 %);  Human (10.9 %);  Bacteria (20.9 %);  All except yeast (74.4 %)
  • similarity to C.albicans agglutinin-like protein
Functional Classification:
  • INTERACTION WITH THE ENVIRONMENT
    ..cellular sensing and response to external stimulus
    ....chemoperception and response
    ......pheromone response, mating-type determination, sex-specific proteins | 189 Entries | Evi | PUBMED
  • PROTEIN WITH BINDING FUNCTION OR COFACTOR REQUIREMENT (structural or catalytic)
    ..protein binding | 392 Entries | Evi | PUBMED
InterPro:
Localization:  SAG1 localization details
  • plasma membrane
 TargetP: Secretory pathway  (RC = 1)
Remarks:
  • a and alpha cells express the complementary cell surface glycoproteins a-agglutinin and alpha- which interact with one another to promote cellular aggregation during mating
  • induction by exposition to pheromone (a-factor) secreted by the opposite mating type cells (type a)
  • N-terminal region has a high proportion of antiparallel beta-sheet, consistent with immunoglobulin-like folds | PUBMED
  • molecular modeling by homology with V-type IG fold domains predicts exposed amino acids | PUBMED
  • molecular characterization of ligand-binding domain delineates amino acids within Ig fold | PUBMED
  • C-terminal half is 50% ser/thr | PUBMED
  • N-glycosylation is not important for adhesion | PUBMED
  • cys97 and cys114 are disulfide bonded | PUBMED
  • cys202 and cys300 are disulfide bonded | PUBMED
  • has at least 2 sites of N-glycosylation | PUBMED
  • all hydroxy amino acids C-terminal to ser282 are glycosylated, forming an extended chain | PUBMED
  • bound to cell surface with a GPI-anchor | PUBMED
  • signal for GPI-anchor is as amino acids 628-650 | PUBMED
  • GPI-anchor is lost after secretion and Sag1p is found covalently linked to beta-glucans at the outer surface of the cell wall | PUBMED
  • GPI anchor is processed in order to link the protein to cell wall polysaccharides | PUBMED
  • N-glycosylation is not important for adhesion
  • C-terminal half is predicted to form an extended chain due to heavy O-glycosylation | PUBMED
  • has putative N-terminal signal sequence | PUBMED
  • a-agglutinin binding activity is in the N-terminal half | PUBMED
  • fragment from amino acids 20-351 is fully active | PUBMED
  • amino acids 200 to 320 (domain III) have weak similarity to immunoglobulin variable region | PUBMED
  • has three predicted immunoglobulin-like domains in the N-terminal region
Phenotypes:  Disruption: SAG1 details
  • viable
___
  • null mutant has loss of aggulutinability | PUBMED
Click for larger Map
Physical Features
Coordinates:444854 - 442902 (C)
Length:1953 nt
Contig:chrX
Exon Coordinates:444854 - 442902
GC Content:39.7 %
Protein Length:650 aa
Isoelectric Point:4.39
Molecular Weight:70339.6 Da
Mature Protein:20-650
Transmembrane Domains:Begin - End / Length (aa)
630 - 649 / 20
1 TM- Domain
Retrieve Sequence: DNA /Protein
Literature | References
CYGD curated Lit.:PubMed
PubMed:Search abstracts
PubMed Central:Search full-text articles
Sequence References:
EMBL: X87611
EMBL: Z49504
EMBL: X87611
PIR: S22835
EMBL: M28164
EMBL: X16861
UniProtKB: P20840
NCBI: NP_012537
Additional Links:
LinkOut:
  • SGD - Saccharomyces Genome Database
  • YGOB - Yeast Gene Order Browser
  • yMGV - yeast Microarray Global Viewer
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