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MIPS Fungal Genomes

mips home MIPS-FUNGI-ADM      FTP
TRK1 Potassium transporter I 
Entry:YJL129c
Alias:J0693
Classification:known protein | 5422 Entries
Feature Type:CDS
Features
   Blast-Simap    DNA VIEW    PROTEIN VIEW

Similarity:
Paralogs (41 %) ;  |  Homologs in   Hemiascomycota (91.2 %);  Basidiomycota (21.9 %);  Fungi (91.2 %);  Eukaryota (91.2 %);  Plants (12.8 %);  Mammalia (9.6 %);  Human (9.6 %);  Bacteria (9.8 %);  All except yeast (91.2 %)

  • similarity to Trk2p, and S.pombe potassium transport protein homolog
  • member of the potassium permease family of the major facilitator superfamily
Functional Classification:
  • INTERACTION WITH THE ENVIRONMENT
    ..homeostasis
    ....homeostasis of cations
    ......homeostasis of metal ions (Na, K, Ca etc.) | 98 Entries | Evi | PUBMED
  • CELLULAR TRANSPORT, TRANSPORT FACILITIES AND TRANSPORT ROUTES
    ..transport routes
    ....cellular import | 150 Entries | Evi
  • CELLULAR TRANSPORT, TRANSPORT FACILITIES AND TRANSPORT ROUTES
    ..transported compounds (substrates)
    ....ion transport
    ......cation transport (H+, Na+, K+, Ca2+ , NH4+, etc.) | 122 Entries | Evi | PUBMED
TC Class:
  • The Uni/Sym/Antiporters
    ..The Uni/Sym/Antiporters
    ....The K+ Transporter (TRK) Family
    ......The K+ Transporter (TRK-2) Subfamily Type2 | 2 Entries | Evi | PUBMED
InterPro:
Localization:  TRK1 localization details
  • plasma membrane
 TargetP: Mitochondrion  (RC = 3)
Remarks:
  • potassium uptake (Trk1p plus Trk2p activity) is activated by glucose and other fermentable sugars and by cytoplasmic acidification or by neomycin | PUBMED
    Phenotypes:  Disruption: TRK1 details
    • viable
    ___
    • trk1 trk2 double null mutants are hypersensitive to channel blocker 4-aminopyridine | PUBMED
    • trk1 trk2 double mutant show normal growth on media containing 100 mM K+, slow growth with 7 mM K+ and no growth with 0.2 mM K+
    • trk1 trk2 double mutant show no growth on pH 3.0 | PUBMED
    • Exhibits sensitivity at 5 generations when grown in 1 M NaCl. | Evi | PUBMED
    • Exhibits sensitivity at 5 generations when grown in minimal +his/leu/ura medium. | Evi | PUBMED
    • Exhibits sensitivity at 5 generations when grown in synthetic complete medium. | Evi | PUBMED
    Suppression:
    • trk1 trk2 double null mutant can be suppressed on K+-limiting medium by gain-of function mutations of HXT1, HXT3 or GAL2 | PUBMED
    • the suppression of trk1 trk2 double null mutant by HXT1, HXT3 or GAL2 gain-of-function mutants is dependent on glucose | PUBMED
    • Arabidopsis KAT1 overexpression confer normal growth to trk1 trk2 double mutant
    • hip1-293 mutation F293 to S | PUBMED
    • trk1 trk2 double null mutant can be suppressed by HOL1-1 dominant mutation | PUBMED
    Click for larger Map
    Physical Features
    Coordinates:177303 - 173596 (C)
    Length:3708 nt
    Contig:chrX
    Exon Coordinates:177303 - 173596
    GC Content:39.1 %
    Protein Length:1235 aa
    Isoelectric Point:8.64
    Molecular Weight:141074 Da
    Transmembrane Domains:Begin - End / Length (aa)
    50 - 72 / 23
    106 - 128 / 23
    778 - 800 / 23
    845 - 867 / 23
    906 - 928 / 23
    972 - 991 / 20
    1079 - 1096 / 18
    1109 - 1131 / 23

    8 TM- Domains
    Retrieve Sequence: DNA /Protein
    Literature | References
    CYGD curated Lit.:PubMed
    PubMed:Search abstracts
    PubMed Central:Search full-text articles
    Sequence References:
    EMBL: Z49404
    EMBL: M21328
    PIR: S05849
    UniProtKB: P12685
    NCBI: NP_012406
    Additional Links:
    LinkOut:
  • SGD - Saccharomyces Genome Database
  • YGOB - Yeast Gene Order Browser
  • yMGV - yeast Microarray Global Viewer

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