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About CYGD


MIPS Fungal Genomes

mips home MIPS-FUNGI-ADM      FTP
PPA1 H+-ATPase 23 KD subunit, vacuolar 
Entry:YHR026w
Alias:VMA16
Classification:known protein | 5422 Entries | Evi | PUBMED
Feature Type:CDS
Features
   Blast-Simap    DNA VIEW    PROTEIN VIEW

Similarity:
Paralogs (24.4 %) ;  |  Homologs in   Hemiascomycota (99.1 %);  Basidiomycota (57.2 %);  Fungi (99.1 %);  Eukaryota (99.1 %);  Plants (46 %);  Mammalia (49.3 %);  Human (48.4 %);  Bacteria (24.4 %);  All except yeast (99.1 %)

  • strong similarity to C.elegans hypothetical protein
  • similarity to other H+-transporting ATPases
  • similarity to V-ATPase proteolipid subunits
Functional Classification:
  • INTERACTION WITH THE ENVIRONMENT
    ..homeostasis
    ....homeostasis of cations
    ......homeostasis of protons | 48 Entries | Evi | PUBMED
  • CELLULAR TRANSPORT, TRANSPORT FACILITIES AND TRANSPORT ROUTES
    ..transport facilities
    ....transport ATPases | 54 Entries | Evi
  • CELLULAR TRANSPORT, TRANSPORT FACILITIES AND TRANSPORT ROUTES
    ..transported compounds (substrates)
    ....ion transport
    ......cation transport (H+, Na+, K+, Ca2+ , NH4+, etc.) | 122 Entries | Evi | PUBMED
  • CELLULAR TRANSPORT, TRANSPORT FACILITIES AND TRANSPORT ROUTES
    ..transported compounds (substrates)
    ....electron transport | 83 Entries | Evi | PUBMED
EC Number:
  • 3.6.3.14: Hydrolases
    ..Acting on Acid Anhydrides
    ....Acting on acid anhydrides; catalysing transmembrane movement of substances
    ......H+-transporting two-sector ATPase | 32 Entries
TC Class:
  • The Driven Transporters
    ..The P-P Bond Hydrolysis - Driven Transporters
    ....The H+ or Na+ Translocating F/V/A-type ATPase (F-ATPase) Superfamily
    ......The H+-translocating V-type ATPase Subfamily | 14 Entries | Evi | PUBMED
InterPro:
Localization:  PPA1 localization details
  • integral membrane / endomembranes (not assigned to a specific membrane)
  • cytoplasm
  • ER
  • vacuole
  • vacuole
    ..vacuolar membrane
 TargetP: Secretory pathway  (RC = 4)
Remarks:
  • Proteolipid of Proton ATPase
  • Glu-108 is required for V-H+-ATPase activity
Phenotypes:  Disruption: PPA1 details
  • lethal
  • viable
___
  • ppa1 disruption mutant fails to grow in YPD medium buffered at pH 7.5, in YPD medium containing 100 mM CaCl2 or in mediumcontaining glycerol as a sole carbon source
Click for larger Map
Physical Features
Coordinates:160837 - 161478 (W)
Length:642 nt
Contig:chrVIII
Exon Coordinates:160837 - 161478
GC Content:41.6 %
Protein Length:213 aa
Isoelectric Point:8.39
Molecular Weight:22595.9 Da
Transmembrane Domains:Begin - End / Length (aa)
20 - 38 / 19
59 - 81 / 23
101 - 123 / 23
143 - 165 / 23
185 - 207 / 23

5 TM- Domains
Retrieve Sequence: DNA /Protein
Literature | References
CYGD curated Lit.:PubMed
PubMed:Search abstracts
PubMed Central:Search full-text articles
Sequence References:
EMBL: U10399
PIR: A34633
UniProtKB: P23968
NCBI: NP_011891
Additional Links:
LinkOut:
  • SGD - Saccharomyces Genome Database
  • YGOB - Yeast Gene Order Browser
  • yMGV - yeast Microarray Global Viewer

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