GSFLogo GSF

 

mipsLogo mips
GenRE
Search ORF/Gene/Description

Chromosome
(chrI - chrXVI,chrMito,2-micron)

Catalogs:
Chromosome View
  I  II  III  IV  V  VI  VII
  VIII  IX  X  XI  XII  XIII
  XIV  XV  XVI  Mito
Other

Search / View

   Advanced Search

   Index Search

MPact protein interaction

Tables, Lists and Reviews

WUBLAST DNA/Protein

About CYGD


MIPS Fungal Genomes

mips home MIPS-FUNGI-ADM      FTP
BEM2 GTPase-activating protein 
Entry:YER155c
Alias:EFR1; ETS1; IPL2; SUP4; SUP9; (TSL1)
Classification:known protein | 5422 Entries | Evi | PUBMED
Feature Type:CDS
Features
   Blast-Simap    DNA VIEW    PROTEIN VIEW

Similarity:
Paralogs (10.6 %) ;  |  Homologs in   Hemiascomycota (93.5 %);  Basidiomycota (22.5 %);  Fungi (93.5 %);  Eukaryota (93.5 %);  Plants (9.3 %);  Mammalia (3.9 %);  Human (5.8 %);  Bacteria (14.9 %);  All except yeast (93.5 %)

  • similarity to human bcr1 and ABR1 protein
Functional Classification:
  • CELLULAR COMMUNICATION/SIGNAL TRANSDUCTION MECHANISM
    ..cellular signalling
    ....enzyme mediated signal transduction
    ......G-protein mediated signal transduction
    ........small GTPase mediated signal transduction | 58 Entries | Evi | PUBMED
  • CELL TYPE DIFFERENTIATION
    ..fungal/microorganismic cell type differentiation
    ....fungal and other eukaryotic cell type differentiation
    ......budding, cell polarity and filament formation | 313 Entries | Evi | PUBMED
  • BIOGENESIS OF CELLULAR COMPONENTS
    ..cytoskeleton/structural proteins
    ....microtubule cytoskeleton | 47 Entries | Evi | PUBMED
  • BIOGENESIS OF CELLULAR COMPONENTS
    ..cytoskeleton/structural proteins
    ....actin cytoskeleton | 96 Entries | Evi | PUBMED
  • CELL CYCLE AND DNA PROCESSING
    ..cell cycle
    ....mitotic cell cycle and cell cycle control | 447 Entries | Evi
  • REGULATION OF METABOLISM AND PROTEIN FUNCTION
    ..regulation of protein activity
    ....enzymatic activity regulation / enzyme regulator
    ......enzyme activator
    ........GTPase activator (GAP) | 32 Entries | Evi | PUBMED
InterPro:
Localization:  BEM2 localization details
  • bud
    ..neck
  • cytoplasm
  • mitochondria
 TargetP: Mitochondrion  (RC = 2)
Remarks:
  • shows no GAP activity on Cdc42p | PUBMED
  • contains a single copy of a Rho-GAP domain near C-terminus (carboxy-terminal 203 residues) | PUBMED
Phenotypes:  Disruption: BEM2 details
  • viable
___
  • null mutants grow poorly at 24C and are inviable at temperatures above 32C | PUBMED
  • null mutants grown at permissive temperatures become large and multinucleate | PUBMED
  • bem2-101 mutants arrest as large, unbudded cells at restrictive temperature | PUBMED
  • bem2-101 mutants are defective in polarized cell surface growth at restrictive temperature | PUBMED
  • bem2-101 mutants show loss of cell polarity and delocalization of chitin deposition associated with failure to organize an assymetric, polarized actin cytoskeleton at the restrictive temperature | PUBMED
  • mutants become polyploid
  • mutants have a weakened cell wall
  • defective in organization of actin cytoskeleton
  • disruptants are caffeine sensitive | PUBMED
  • rom2 delta bem2 delta double mutants are more sensitive to benomyl than either single mutant | PUBMED
___
  • Conditional phenotypes
    ..Heat-sensitivity (ts) | 313 Entries
  • Cell morphology and organelle mutants
    ..Cell wall mutants
    ....other cell wall mutants | 73 Entries
  • Cell morphology and organelle mutants
    ..Cytoskeleton mutants
    ....Tubulin cytoskeleton mutants
    ......benomyl sensitivity | 33 Entries
  • Cell morphology and organelle mutants
    ..Cytoskeleton mutants
    ....Actin cytoskeleton mutants | 48 Entries
  • Sensitivity to aminoacid analogs and other drugs
    ..Caffeine
    ....Caffeine sensitivity | 34 Entries
Suppression:
  • mutation in CDC55 acts as an extragenic suppressor of bem2 | PUBMED
  • mutants can be partially suppressed by overexpression of RHO1 and RHO2 | PUBMED
  • the suppression of bem2 mutations by RHO1 and RHO2 overexpression is additive | PUBMED
  • the temperature sensitive growth phenotype, but not the morphological defects of bem2 mutants can be suppressed by grr1 mutations and by ssd1/srk1 mutations | PUBMED
  • bem2 mutant phenotypes are suppressed by overexpression of the GAP domain from Bem2p and human Bcr | PUBMED
  • bem2 mutant phenotypes cannot be suppressed by overexpression of RHO3, RHO4, CDC42, PPH3, PPH21, PPH22 or deletion of PPH22 | PUBMED
  • bem2 mutant phenotypes can be suppressed by SRK1 | PUBMED
  • high copy number GIC1 plasmid could suppress the Ts-minus phenotype of bem2-101 and bem2 null mutant cells at 35C and 33C | PUBMED
Click for larger Map
Physical Features
Coordinates:482843 - 476340 (C)
Length:6504 nt
Contig:chrV
Exon Coordinates:482843 - 476340
GC Content:36 %
Protein Length:2167 aa
Isoelectric Point:8.53
Molecular Weight:245431 Da
Retrieve Sequence: DNA /Protein
Literature | References
CYGD curated Lit.:PubMed
PubMed:Search abstracts
PubMed Central:Search full-text articles
Sequence References:
EMBL: U18917
EMBL: Z35159
EMBL: L33832
PIR: S50658
UniProtKB: P39960
NCBI: NP_011082
Additional Links:
CYGD Pathways:
  • Model of the Yeast cell integrity signal transduction pathways
  • LinkOut:
  • SGD - Saccharomyces Genome Database
  • YGOB - Yeast Gene Order Browser
  • yMGV - yeast Microarray Global Viewer

  • To submit new information about this entry, please write to

    © 2003 GSF - Forschungszentrum für Umwelt und Gesundheit, GmbH Ingolstädter Landstraße 1, D-85764 Neuherberg

    Imprint

    Disclaimer:
    MIPS Databases and associated information are protected by copyright. This server and its associated data and services are for academic, non-commercial use only. The GSF has no liability for the use of results, data or information which have been provided through this server. Neither the use for commercial purposes, nor the redistribution of MIPS database files to third parties nor the distribution of parts of files or derivative products to any third parties is permitted. Commercial users may contact the distributor Biomax Informatics AG.