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MPact protein interaction

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About CYGD

MIPS Fungal Genomes

mips home MIPS-FUNGI-ADM      FTP
GCN4 Transcriptional activator of amino acid biosynthetic genes 
Alias:AAS3; ARG9
Classification:known protein | 5422 Entries | Evi | PUBMED
Feature Type:CDS
   Blast-Simap    DNA VIEW    PROTEIN VIEW

Paralogs (19.4 %) ;  |  Homologs in   Hemiascomycota (93.3 %);  Basidiomycota (19.2 %);  Fungi (93.3 %);  Eukaryota (93.3 %);  Plants (18 %);  Mammalia (25.7 %);  Human (24.1 %);  Bacteria (19.9 %);  All except yeast (93.3 %)

  • weak similarity to N.crassa cross-pathway control protein 1 and S.canaria immediate-early protein
  • member of the bZIP family
Functional Classification:
    ..amino acid metabolism
    ....regulation of amino acid metabolism | 33 Entries | Evi
    ..nucleic acid binding
    ....DNA binding | 159 Entries | Evi | PUBMED
    ..RNA synthesis
    ....mRNA synthesis
    ......transcriptional control
    ........transcription activation | 42 Entries | Evi | PUBMED
Localization:  GCN4 localization details
  • cytoplasm
  • nucleus
  • a Gcn2p-independent mechanism also regulates Gcn4p translation
  • translational induction can occur by a pathway independent of ser51 phosphorylation on Sui2p
  • expression is stimulated independent of nutritional conditions at the translational level by overexpression of a mutant valyl-tRNA a ser-tRNA and the small RNA component of ribonuclease MRP
  • expression is stimulated at the translational level by overexpression TPK2
  • gcd11-D6N mutant show a growth phenotype and about 14-fold increase in GCN4 expression
  • Gcn4p requires the Ada2p Gcn5p and Ada3 co-activator complex to exert part of its activation potential
  • Gcn4p and Bas1p/Bas2p are able to act simultaneously through the same DNA sequence in vivo (HIS7 promoter)
  • recognizes ATGAC/GTCAT
  • increases 10-fold after starvation for various amino acids or purines
  • translation is regulated by 4 upstream ORFs, of which ORF1 and ORF2 are most important
  • upstream ORF1 is a positive regulator because it has a sequence that allows ribosome to stay bound after reaching the termination codon
  • upstream ORF4 is a negative regulator because if initiation takes place there, the ribosome will release upon termination and will not translate Gcn4p
  • in low amino acid conditions, Gcn2p phosphorylates eIF2alpha inhibiting function of eIF2B and depleting pool of GTP-bound eIF2 causing delayed reinitiation after translating ORF1
  • in high amino acid conditions, reinitiation is rapid, ORF4 is translated, and Gcn4p is not produced
  • eIF3 is involved in Gcn4p regulation
  • degradation in rich medium is rapid and ubiquitin-dependent degradation during amino acid starvation is greatly reduced
  • rapid degradation depends on PEST sequences
  • degradation requires Cdc34p and Rad6p and these can ubiquitinate in vitro
  • degradation is by phosphorylation-dependent ubiquitination
  • has a fos/jun DNA-binding homology domain
  • has acidic activation domain in the middle region (CAAD)
  • acidic activation domain has some critical hydrophobic residues, especially try and trp
  • DNA-binding and bZIP dimerization domains are located at the C-terminus
  • the N-terminus contains at least seven segments of aromatic or bulky hydrophobic residues important for transcriptional activation
  • accumulates in phosphorylated form in cdc34 mutants
Phenotypes:  Disruption: GCN4 details
  • viable
  • Exhibits sensitivity at 15 generations when grown in minimal +his/leu/ura medium. | Evi | PUBMED
  • Exhibits sensitivity at 5 generations when grown in synthetic complete - trp medium. | Evi | PUBMED
Click for larger Map
Physical Features
Coordinates:139763 - 138918 (C)
Length:846 nt
Exon Coordinates:139763 - 138918
GC Content:41.1 %
Protein Length:281 aa
Isoelectric Point:5.08
Molecular Weight:31310.1 Da
Retrieve Sequence: DNA /Protein
Literature | References
CYGD curated Lit.:PubMed
PubMed:Search abstracts
PubMed Central:Search full-text articles
Sequence References:
EMBL: K02205
EMBL: U18530
EMBL: K02649
PIR: A03605
UniProtKB: P03069
NCBI: NP_010907
Additional Links:
  • SGD - Saccharomyces Genome Database
  • YGOB - Yeast Gene Order Browser
  • yMGV - yeast Microarray Global Viewer

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