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MPact protein interaction

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About CYGD

MIPS Fungal Genomes

mips home MIPS-FUNGI-ADM      FTP
INO2 Basic helix-loop-helix (BHLH) transcription factor 
Alias:DIE1; YD9727.18; (SCS1)
Classification:known protein | 5422 Entries | Evi | PUBMED
Feature Type:CDS
   Blast-Simap    DNA VIEW    PROTEIN VIEW

Paralogs (11.6 %) ;  |  Homologs in   Hemiascomycota (88.5 %);  Basidiomycota (18.1 %);  Fungi (88.5 %);  Eukaryota (88.5 %);  Plants (15.6 %);  Mammalia (10.3 %);  Human (12.8 %);  Bacteria (15.2 %);  All except yeast (88.5 %)

  • weak similarity to hypothetical C.elegans protein
Functional Classification:
    ..lipid, fatty acid and isoprenoid metabolism
    ....regulation of lipid, fatty acid and isoprenoid metabolism | 26 Entries | Evi
    ..lipid, fatty acid and isoprenoid metabolism
    ....membrane lipid metabolism
    ......phospholipid metabolism | 68 Entries | Evi | PUBMED
Localization:  INO2 localization details
  • nucleus
  • has effect of preventing repression by inositol + choline
  • degree of INO1 and CHO1 expression in absence of inositol correlates with transcription of INO2, but only up to a maximum level that may be determined by the amount of Ino4p
  • inositol leads to repression of Ino2p target genes through reducing level of INO2 expression and through an independent mechanism involving OPI1
  • Opi1p dependent repression of INO2 is mediated by the ICRE in the promoter | PUBMED
  • low level expression of INO2 is sufficient for function | PUBMED
  • N-terminal domain contains two separable transcription activation domains, TAD1 and TAD2
Phenotypes:  Disruption: INO2 details
  • viable
  • null mutants grown in inositol/choline have defect in polarized growth leading to larger cells with aberrant shapes
  • null mutants have defects of bud formation and nuclear segregation
  • diploid null mutant fails to sporulate
  • mutants are defective in uptake and metabolism of glycerophosphoinositol | PUBMED
  • Exhibits sensitivity at 15 generations when grown in minimal +his/leu/ura medium. | Evi | PUBMED
  • Mating and sporulation defects
    ..Sporulation efficiency | 112 Entries
  • Carbohydrate and lipid biosynthesis defects
    ..other carbohydrate and lipid biosynthesis defects | 36 Entries
  • overproduction leads to almost constitutive expression of inositol/choline response element (ICRE)-activated genes
  • overexpression of INO1 or INO2 rescues the inositol auxotrophy of scs2delta strains | PUBMED
Click for larger Map
Physical Features
Coordinates:699465 - 698551 (C)
Length:915 nt
Exon Coordinates:699465 - 698551
GC Content:43.5 %
Protein Length:304 aa
Isoelectric Point:5.78
Molecular Weight:34234.3 Da
Retrieve Sequence: DNA /Protein
Literature | References
CYGD curated Lit.:PubMed
PubMed:Search abstracts
PubMed Central:Search full-text articles
Sequence References:
EMBL: X66066
EMBL: Z48758
PIR: S21570
UniProtKB: P26798
NCBI: NP_010408
Additional Links:
  • SGD - Saccharomyces Genome Database
  • YGOB - Yeast Gene Order Browser
  • yMGV - yeast Microarray Global Viewer

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