General Information:

Id: 6,616
Diseases: Pseudomonas aeruginosa diseases
pathogen-host system
Pseudomonas aeruginosa/mammalia
human mucoid bronchial epithelial cell line, H292 treated with pyocyanin
article
Reference: Rada B et al.(2011) Reactive oxygen species mediate inflammatory cytokine release and EGFR-dependent mucin secretion in airway epithelial cells exposed to Pseudomonas pyocyanin Mucosal Immunol 4: 158-171 [PMID: 20962773]

Interaction Information:

Comment Pyocyanin induces MUC5AC and MUC2 in bronchiolar epithelial cells. Quantitative real-time PCR, qualitative RT-PCR and MUC5AC ELISA showed time- and dose-dependent induction of MUC5AC and MUC2 mRNA and proteins by pyocyanin in TBECs (tracheobronchiolar epithelial cells) .
Formal Description
Interaction-ID: 63004

drug/chemical compound

Pyocyanine

increases_expression of

gene/protein

MUC5AC

Comment Pyocyanin induces MUC5AC and MUC2 in bronchiolar epithelial cells. Quantitative real-time PCR, qualitative RT-PCR and MUC5AC ELISA showed time- and dose-dependent induction of MUC5AC and MUC2 mRNA and proteins by pyocyanin in TBECs (tracheobronchiolar epithelial cells) .
Formal Description
Interaction-ID: 63005

drug/chemical compound

Pyocyanine

increases_quantity of

gene/protein

MUC5AC

Comment Pyocyanin induces MUC5AC and MUC2 in bronchiolar epithelial cells. Quantitative real-time PCR, qualitative RT-PCR and MUC5AC ELISA showed time- and dose-dependent induction of MUC5AC and MUC2 mRNA and proteins by pyocyanin in TBECs (tracheobronchiolar epithelial cells) .
Formal Description
Interaction-ID: 63006

drug/chemical compound

Pyocyanine

increases_expression of

gene/protein

MUC2

Drugbank entries Show/Hide entries for MUC2
Comment Pyocyanin induces MUC5AC and MUC2 in bronchiolar epithelial cells. Quantitative real-time PCR, qualitative RT-PCR and MUC5AC ELISA showed time- and dose-dependent induction of MUC5AC and MUC2 mRNA and proteins by pyocyanin in TBECs (tracheobronchiolar epithelial cells) .
Formal Description
Interaction-ID: 63007

drug/chemical compound

Pyocyanine

increases_quantity of

gene/protein

MUC2

Drugbank entries Show/Hide entries for MUC2
Comment MUC5AC, MUC5B and MUC2 are the major components of the airway mucus (cited information).
Formal Description
Interaction-ID: 63008

gene/protein

MUC5AC

increases_activity of

process

mucus secretion

Comment MUC5AC, MUC5B and MUC2 are the major components of the airway mucus (cited information).
Formal Description
Interaction-ID: 63009

gene/protein

MUC2

increases_activity of

process

mucus secretion

Drugbank entries Show/Hide entries for MUC2
Comment MUC5AC, MUC5B and MUC2 are the major components of the airway mucus (cited information).
Formal Description
Interaction-ID: 63010

gene/protein

MUC5B

increases_activity of

process

mucus secretion

Comment Five cytokines (IL-1-alpha, IL-1-beta, IL-6, IL-8, TNF-alpha) and two EGFR ligands (TGF-alpha, HB-EGF) were strongly up-regulated by pyocyaninin TBECs (tracheobronchiolar epithelial cells).
Formal Description
Interaction-ID: 63011

drug/chemical compound

Pyocyanine

increases_expression of

gene/protein

IL1A

Comment Five cytokines (IL-1-alpha, IL-1-beta, IL-6, IL-8, TNF-alpha) and two EGFR ligands (TGF-alpha, HB-EGF) were strongly up-regulated by pyocyaninin TBECs (tracheobronchiolar epithelial cells).
Formal Description
Interaction-ID: 63012

drug/chemical compound

Pyocyanine

increases_expression of

gene/protein

IL1B

Drugbank entries Show/Hide entries for IL1B
Comment Five cytokines (IL-1-alpha, IL-1-beta, IL-6, IL-8, TNF-alpha) and two EGFR ligands (TGF-alpha, HB-EGF) were strongly up-regulated by pyocyaninin TBECs (tracheobronchiolar epithelial cells).
Formal Description
Interaction-ID: 63013

drug/chemical compound

Pyocyanine

increases_expression of

gene/protein

IL6

Drugbank entries Show/Hide entries for IL6
Comment Five cytokines (IL-1-alpha, IL-1-beta, IL-6, IL-8, TNF-alpha) and two EGFR ligands (TGF-alpha, HB-EGF) were strongly up-regulated by pyocyaninin TBECs (tracheobronchiolar epithelial cells).
Formal Description
Interaction-ID: 63014

drug/chemical compound

Pyocyanine

increases_expression of

gene/protein

TNF

Drugbank entries Show/Hide entries for TNF
Comment Five cytokines (IL-1-alpha, IL-1-beta, IL-6, IL-8, TNF-alpha) and two EGFR ligands (TGF-alpha, HB-EGF) were strongly up-regulated by pyocyaninin TBECs (tracheobronchiolar epithelial cells).
Formal Description
Interaction-ID: 63015

drug/chemical compound

Pyocyanine

increases_expression of

gene/protein

TGFA

Comment Five cytokines (IL-1-alpha, IL-1-beta, IL-6, IL-8, TNF-alpha) and two EGFR ligands (TGF-alpha, HB-EGF) were strongly up-regulated by pyocyaninin TBECs (tracheobronchiolar epithelial cells).
Formal Description
Interaction-ID: 63016

drug/chemical compound

Pyocyanine

increases_expression of

gene/protein

HBEGF

Comment Five cytokines (IL-1-alpha, IL-1-beta, IL-6, IL-8, TNF-alpha) and two EGFR ligands (TGF-alpha, HB-EGF) were strongly up-regulated by pyocyaninin TBECs (tracheobronchiolar epithelial cells).
Formal Description
Interaction-ID: 63017

drug/chemical compound

Pyocyanine

increases_expression of

gene/protein

CXCL8

Comment Significantly enhanced amounts of IL-1-beta, TGF-alpha, TNF-alpha and IL-6 were detected by ELISA in the supernatant of pyocyanin-treated cells compared to untreated cells.
Formal Description
Interaction-ID: 63018

drug/chemical compound

Pyocyanine

increases_quantity of

gene/protein

IL1B

Drugbank entries Show/Hide entries for IL1B
Comment Significantly enhanced amounts of IL-1-beta, TGF-alpha, TNF-alpha and IL-6 were detected by ELISA in the supernatant of pyocyanin-treated cells compared to untreated cells.
Formal Description
Interaction-ID: 63019

drug/chemical compound

Pyocyanine

increases_quantity of

gene/protein

TGFA

Comment Significantly enhanced amounts of IL-1-beta, TGF-alpha, TNF-alpha and IL-6 were detected by ELISA in the supernatant of pyocyanin-treated cells compared to untreated cells.
Formal Description
Interaction-ID: 63020

drug/chemical compound

Pyocyanine

increases_quantity of

gene/protein

TNF

Drugbank entries Show/Hide entries for TNF
Comment Significantly enhanced amounts of IL-1-beta, TGF-alpha, TNF-alpha and IL-6 were detected by ELISA in the supernatant of pyocyanin-treated cells compared to untreated cells.
Formal Description
Interaction-ID: 63021

drug/chemical compound

Pyocyanine

increases_quantity of

gene/protein

IL6

Drugbank entries Show/Hide entries for IL6
Comment IL-1-beta, IL-6, TGF-alpha and TNF-alpha appear to signal upstream of EGFR to induce release of MUC5AC.
Formal Description
Interaction-ID: 63022

gene/protein

IL1B

increases_quantity of

gene/protein

MUC5AC

Drugbank entries Show/Hide entries for IL1B
Comment IL-1-beta, IL-6, TGF-alpha and TNF-alpha appear to signal upstream of EGFR to induce release of MUC5AC.
Formal Description
Interaction-ID: 63023

gene/protein

IL6

increases_quantity of

gene/protein

MUC5AC

Drugbank entries Show/Hide entries for IL6
Comment IL-1-beta, IL-6, TGF-alpha and TNF-alpha appear to signal upstream of EGFR to induce release of MUC5AC.
Formal Description
Interaction-ID: 63024

gene/protein

TNF

increases_quantity of

gene/protein

MUC5AC

Drugbank entries Show/Hide entries for TNF
Comment IL-1-beta, IL-6, TGF-alpha and TNF-alpha appear to signal upstream of EGFR to induce release of MUC5AC.
Formal Description
Interaction-ID: 63025

gene/protein

TGFA

increases_quantity of

gene/protein

MUC5AC

Comment The microarray data confirmed by real-time PCR shows that 6 of 10 PA-associated inflammatory mediators (G-CSF, IL-6, IL-1-beta, IL-8, TNF-alpha, LTB4) and 6 of 14 additional CF-associated genes (GM-CSF, CXCL1, IL-23, IL-1Ra, SAA1, SAA2) were up-regulated in TBECs by pyocyanin. This suggests that pyocyanin’s effect on bronchiolar epithelial cells can be a major contributor to the initiation and maintainance of the hyperinflammatory state in CF airways.
Formal Description
Interaction-ID: 63026

drug/chemical compound

Pyocyanine

increases_expression of

gene/protein

CSF3

Comment The microarray data confirmed by real-time PCR shows that 6 of 10 PA-associated inflammatory mediators (G-CSF, IL-6, IL-1-beta, IL-8, TNF-alpha, LTB4) and 6 of 14 additional CF-associated genes (GM-CSF, CXCL1, IL-23, IL-1Ra, SAA1, SAA2) were up-regulated in TBECs by pyocyanin. This suggests that pyocyanin’s effect on bronchiolar epithelial cells can be a major contributor to the initiation and maintainance of the hyperinflammatory state in CF airways.
Formal Description
Interaction-ID: 63027

drug/chemical compound

Pyocyanine

increases_expression of

gene/protein

CSF2

Comment The microarray data confirmed by real-time PCR shows that 6 of 10 PA-associated inflammatory mediators (G-CSF, IL-6, IL-1-beta, IL-8, TNF-alpha, LTB4) and 6 of 14 additional CF-associated genes (GM-CSF, CXCL1, IL-23, IL-1Ra, SAA1, SAA2) were up-regulated in TBECs by pyocyanin. This suggests that pyocyanin’s effect on bronchiolar epithelial cells can be a major contributor to the initiation and maintainance of the hyperinflammatory state in CF airways.
Formal Description
Interaction-ID: 63028

drug/chemical compound

Pyocyanine

increases_expression of

gene/protein

CXCL1

Comment The microarray data confirmed by real-time PCR shows that 6 of 10 PA-associated inflammatory mediators (G-CSF, IL-6, IL-1-beta, IL-8, TNF-alpha, LTB4) and 6 of 14 additional CF-associated genes (GM-CSF, CXCL1, IL-23, IL-1Ra, SAA1, SAA2) were up-regulated in TBECs by pyocyanin. This suggests that pyocyanin’s effect on bronchiolar epithelial cells can be a major contributor to the initiation and maintainance of the hyperinflammatory state in CF airways.
Formal Description
Interaction-ID: 63029

drug/chemical compound

Pyocyanine

increases_expression of

gene/protein

IL23A

Comment The microarray data confirmed by real-time PCR shows that 6 of 10 PA-associated inflammatory mediators (G-CSF, IL-6, IL-1-beta, IL-8, TNF-alpha, LTB4) and 6 of 14 additional CF-associated genes (GM-CSF, CXCL1, IL-23, IL-1Ra, SAA1, SAA2) were up-regulated in TBECs by pyocyanin. This suggests that pyocyanin’s effect on bronchiolar epithelial cells can be a major contributor to the initiation and maintainance of the hyperinflammatory state in CF airways.
Formal Description
Interaction-ID: 63030

drug/chemical compound

Pyocyanine

increases_expression of

gene/protein

IL1RN

Comment The microarray data confirmed by real-time PCR shows that 6 of 10 PA-associated inflammatory mediators (G-CSF, IL-6, IL-1-beta, IL-8, TNF-alpha, LTB4) and 6 of 14 additional CF-associated genes (GM-CSF, CXCL1, IL-23, IL-1Ra, SAA1, SAA2) were up-regulated in TBECs by pyocyanin. This suggests that pyocyanin’s effect on bronchiolar epithelial cells can be a major contributor to the initiation and maintainance of the hyperinflammatory state in CF airways.
Formal Description
Interaction-ID: 63031

drug/chemical compound

Pyocyanine

increases_expression of

gene/protein

SAA1

Drugbank entries Show/Hide entries for SAA1
Comment Pyocyanin strongly induced transcription of of IL-24.
Formal Description
Interaction-ID: 63032

drug/chemical compound

Pyocyanine

increases_expression of

gene/protein

IL24

Comment Pyocyanin strongly induced transcription of one IL-receptor (Interleukin 1 receptor, type 2 (IL1R2)), three chemokines (CXCL2, CXCL3, CCL20) and one chemokine receptor (CXCR4).
Formal Description
Interaction-ID: 63033

drug/chemical compound

Pyocyanine

increases_expression of

gene/protein

IL1R2

Comment Pyocyanin strongly induced transcription of one IL-receptor (Interleukin 1 receptor, type 2 (IL1R2)), three chemokines (CXCL2, CXCL3, CCL20) and one chemokine receptor (CXCR4).
Formal Description
Interaction-ID: 63036

drug/chemical compound

Pyocyanine

increases_expression of

gene/protein

CXCL2

Comment Pyocyanin strongly induced transcription of one IL-receptor (Interleukin 1 receptor, type 2 (IL1R2)), three chemokines (CXCL2, CXCL3, CCL20) and one chemokine receptor (CXCR4).
Formal Description
Interaction-ID: 63037

drug/chemical compound

Pyocyanine

increases_expression of

gene/protein

CXCL3

Comment Pyocyanin strongly induced transcription of one IL-receptor (Interleukin 1 receptor, type 2 (IL1R2)), three chemokines (CXCL2, CXCL3, CCL20) and one chemokine receptor (CXCR4).
Formal Description
Interaction-ID: 63038

drug/chemical compound

Pyocyanine

increases_expression of

gene/protein

CCL20

Comment Pyocyanin strongly induced transcription of one IL-receptor (Interleukin 1 receptor, type 2 (IL1R2)), three chemokines (CXCL2, CXCL3, CCL20) and one chemokine receptor (CXCR4).
Formal Description
Interaction-ID: 63040

drug/chemical compound

Pyocyanine

increases_expression of

gene/protein

CXCR4

Drugbank entries Show/Hide entries for CXCR4
Comment Pyocyanin induced transcription of SOD2, involved in response to oxidative stress.
Formal Description
Interaction-ID: 63043

drug/chemical compound

Pyocyanine

increases_expression of

gene/protein

SOD2

Drugbank entries Show/Hide entries for SOD2
Comment The transcription of catalase was found to be slightly decreased upon pyocyanin exposure.
Formal Description
Interaction-ID: 63044

drug/chemical compound

Pyocyanine

decreases_expression of

gene/protein

CAT

Drugbank entries Show/Hide entries for CAT
Comment Growth differentiation factor-15 (GDF-15), a stress-induced cytokine, was strongly up-regulated by pyocyanin.
Formal Description
Interaction-ID: 63046

drug/chemical compound

Pyocyanine

increases_expression of

gene/protein

GDF15

Comment Intracellular production of ROS is responsible for pyocyanin-induced mucin secretion.
Formal Description
Interaction-ID: 63055

drug/chemical compound

Reactive oxygen species

increases_activity of

process

mucus secretion