General Information:

Id: 5,267
Diseases: Pseudomonas aeruginosa diseases
pathogen-host system
Pseudomonas aeruginosa/mammalia
Rat pulmonary microvascular endothelial cells (PMVECs), infected with P. aeruginosa strain 388
article
Reference: Balczon R et al.(2013) Pseudomonas aeruginosa exotoxin Y-mediated tau hyperphosphorylation impairs microtubule assembly in pulmonary microvascular endothelial cells PLoS ONE 8 [PMID: 24023939]

Interaction Information:

Comment ExoY activity causes a decrease in microtubules, as shown by infection of PMVECs with P.aeruginosa expressing either non-functional K81M mutant ExoY or wild type ExoY.
Formal Description
Interaction-ID: 51349

gene/protein

exoY

decreases_quantity of

cellular component

microtubule cytoskeleton

Comment ExoY disrupts microtubule assembly. Microtubule assembly was depressed in cells infected with P. aeruginosa ExoY(+) when compared to control cells and cells infected with P. aeruginosa non-functional mutant ExoY(K81M)
Formal Description
Interaction-ID: 51359

gene/protein

exoY

decreases_activity of

Comment ExoY induces Tau Ser-214 phosphorylation necessary to Impair microtubule reassembl.
Formal Description
Interaction-ID: 51371

gene/protein

exoY

increases_phosphorylation of

gene/protein

MAPT

at Ser-214
Comment P. aeruginosa toxin ExoY, which is an adenylyl and guanylyl cyclase molecule lacking membrane anchoring domains, decreased levels of microtubules in PMVECs and induced barrier breakdown by generating cyclic nucleotides (cAMP and/or cGMP) within a cytosolic domain.
Formal Description
Interaction-ID: 51372

gene/protein

exoY

decreases_activity of

Comment P. aeruginosa ExoY induces Tau (Mapt) Ser-214 phosphorylation necessary to impair microtubule reassembly. In cells expressing the non-phosphorylatable S214A mutant hTau, ExoY had no effects on microtubule assembly and disassembly following infection. This demonstrates that phosphorylation of Tau on Ser-214 can be used by microvascular endothelial cells for modulating microtubule dynamics.
Formal Description
Interaction-ID: 51481

gene/protein

MAPT

decreases_activity of

if MAPT is phosphorylatyed at Ser-214
Comment ExoY activity does not noticeably affect microtubule assembly from PMVEC centrosomes, as shown by infection with P. aeruginosa expressing either the non-functional mutant ExoY(K81M) or ExoY(+).
Formal Description
Interaction-ID: 51489

gene/protein

exoY

NOT affects_activity of

Comment Phosphorylation of Tau disrupts microtubules in PMVECs leading to barrier disruption contributing to the pathologies associated with Pseudomonas infections.
Formal Description
Interaction-ID: 61820