General Information:

Id: 4,943 (click here to show other Interactions for entry)
Diseases: Pseudomonas aeruginosa diseases
pathogen-host system
Pseudomonas aeruginosa/mammalia
primary endothelial cell types, HUVEC, HMVEC and BAEC cells, infected with P. aeruginosa strain PAO1 (PAO1F)
article
Reference: Huber P et al.Sequential inactivation of Rho GTPases and Lim kinase by Pseudomonas aeruginosa toxins ExoS and ExoT leads to endothelial monolayer breakdown [PMID: 23974244]

Interaction Information:

Comment ExoS and ExoT, but not by ExoY induce cell retraction in primary endothelial cells derived from veins (HUVEC), capillaries (HMVEC), and arteries (BAEC), a feature associated with disruption of the actin cytoskeleton and focal adhesions. Quantification of cell retraction shows that Pa-S (PAO1F exoT(-) exoY(-)) was more toxic than Pa-T (PAO1F exoS(-) exoY(-)
Formal Description
Interaction-ID: 48851

gene/protein

exoT

decreases_activity of

in primary endothelial cells
Comment ExoS and ExoT, but not by ExoY induce cell retraction in primary endothelial cells derived from veins (HUVEC), capillaries (HMVEC), and arteries (BAEC), a feature associated with disruption of the actin cytoskeleton and focal adhesions. Quantification of cell retraction shows that Pa-S (PAO1F exoT(-) exoY(-)) was more toxic than Pa-T (PAO1F exoS(-) exoY(-)
Formal Description
Interaction-ID: 48853

gene/protein

exoT

decreases_activity of

in primary endothelial cells
Comment ExoS and ExoT, but not by ExoY induce cell retraction in primary endothelial cells derived from veins (HUVEC), capillaries (HMVEC), and arteries (BAEC), a feature associated with disruption of the actin cytoskeleton and focal adhesions. Quantification of cell retraction shows that Pa-S (PAO1F exoT(-) exoY(-)) was more toxic than Pa-T (PAO1F exoS(-) exoY(-)
Formal Description
Interaction-ID: 48856

gene/protein

exoT

decreases_activity of

in primary endothelial cells
Comment ExoS and ExoT affect cofilin and Lim kinase phosphorylation levels. Cofilin is an actin severing and depolymerization factor that is inactivated by phosphorylation. The infection of HUVEC cells with Pa-WT, Pa-S (PAO1F exoT(-) exoY(-)) or Pa-T (PAO1F exoS(-) exoY(-)) induces a dephosphorylation of cofilin.
Formal Description
Interaction-ID: 48862

gene/protein

exoT

decreases_phosphorylation of

gene/protein

CFL1

in primary endothelial cells
Drugbank entries Show/Hide entries for CFL1
Comment FAK (focal adhesion kinase, PTK2) inactivation was triggered by ExoS and ExoT and paralleled focal adhesion disruption.
Formal Description
Interaction-ID: 48864

gene/protein

exoT

decreases_activity of

gene/protein

PTK2

in primary endothelial cells
Drugbank entries Show/Hide entries for PTK2
Comment ExoS and ExoT affect cofilin and Lim kinase phosphorylation levels. The phosphorylation levels of Lim kinase (LIMK1, LIMK2) progressively decreased when HUVECs were infected with Pa-WT, Pa-T (PAO1F exoS(-) exoY(-)), or Pa-S (PAO1F exoT(-) exoY(-)). Lim kinase has a potent role in maintaining the actin cytoskeleton integrity in endothelial cells. .
Formal Description
Interaction-ID: 48868

gene/protein

exoT

decreases_phosphorylation of

gene/protein

LIMK1

in primary endothelial cells
Comment ExoS and ExoT affect cofilin and Lim kinase phosphorylation levels. The phosphorylation levels of Lim kinase (LIMK1, LIMK2) progressively decreased when HUVECs were infected with Pa-WT, Pa-T (PAO1F exoS(-) exoY(-)), or Pa-S (PAO1F exoT(-) exoY(-)). Lim kinase has a potent role in maintaining the actin cytoskeleton integrity in endothelial cells. .
Formal Description
Interaction-ID: 48869

gene/protein

exoT

decreases_phosphorylation of

gene/protein

LIMK2

in primary endothelial cells
Comment ExoT or ExoS individually induced a decrease in RhoA-activity at 3-h post-infection in HUVEC cells.
Formal Description
Interaction-ID: 48875

gene/protein

exoT

decreases_activity of

gene/protein

RHOA

in primary endothelial cells
Drugbank entries Show/Hide entries for RHOA
Comment ExoT or ExoS induced a decrease in Rac1-activity at 3-h post-infection in HUVEC cells.
Formal Description
Interaction-ID: 48877

gene/protein

exoT

decreases_activity of

gene/protein

RAC1

in primary endothelial cells
Drugbank entries Show/Hide entries for RAC1
Comment ExoT or ExoS induced a decrease in Cdc42-activity at 3-h post-infection in HUVEC cells.
Formal Description
Interaction-ID: 48879

gene/protein

exoT

decreases_activity of

gene/protein

CDC42

in primary endothelial cells
Drugbank entries Show/Hide entries for CDC42