General Information:

Id: 4,943
Diseases: Pseudomonas aeruginosa diseases
pathogen-host system
Pseudomonas aeruginosa/mammalia
primary endothelial cell types, HUVEC, HMVEC and BAEC cells, infected with P. aeruginosa strain PAO1 (PAO1F)
article
Reference: Huber P et al.Sequential inactivation of Rho GTPases and Lim kinase by Pseudomonas aeruginosa toxins ExoS and ExoT leads to endothelial monolayer breakdown [PMID: 23974244]

Interaction Information:

Comment ExoS and ExoT, but not by ExoY induce cell retraction in primary endothelial cells derived from veins (HUVEC), capillaries (HMVEC), and arteries (BAEC), a feature associated with disruption of the actin cytoskeleton and focal adhesions. Quantification of cell retraction shows that Pa-S (PAO1F exoT(-) exoY(-)) was more toxic than Pa-T (PAO1F exoS(-) exoY(-)).
Formal Description
Interaction-ID: 48850

gene/protein

exoS

decreases_activity of

in primary endothelial cells
Comment ExoS and ExoT, but not by ExoY induce cell retraction in primary endothelial cells derived from veins (HUVEC), capillaries (HMVEC), and arteries (BAEC), a feature associated with disruption of the actin cytoskeleton and focal adhesions. Quantification of cell retraction shows that Pa-S (PAO1F exoT(-) exoY(-)) was more toxic than Pa-T (PAO1F exoS(-) exoY(-)
Formal Description
Interaction-ID: 48851

gene/protein

exoT

decreases_activity of

in primary endothelial cells
Comment ExoS and ExoT, but not by ExoY induce cell retraction in primary endothelial cells derived from veins (HUVEC), capillaries (HMVEC), and arteries (BAEC), a feature associated with disruption of the actin cytoskeleton and focal adhesions. Quantification of cell retraction shows that Pa-S (PAO1F exoT(-) exoY(-)) was more toxic than Pa-T (PAO1F exoS(-) exoY(-)
Formal Description
Interaction-ID: 48852

gene/protein

exoS

decreases_activity of

in primary endothelial cells
Comment ExoS and ExoT, but not by ExoY induce cell retraction in primary endothelial cells derived from veins (HUVEC), capillaries (HMVEC), and arteries (BAEC), a feature associated with disruption of the actin cytoskeleton and focal adhesions. Quantification of cell retraction shows that Pa-S (PAO1F exoT(-) exoY(-)) was more toxic than Pa-T (PAO1F exoS(-) exoY(-)
Formal Description
Interaction-ID: 48853

gene/protein

exoT

decreases_activity of

in primary endothelial cells
Comment ExoS and ExoT, but not by ExoY induce cell retraction in primary endothelial cells derived from veins (HUVEC), capillaries (HMVEC), and arteries (BAEC), a feature associated with disruption of the actin cytoskeleton and focal adhesions. Quantification of cell retraction shows that Pa-S (PAO1F exoT(-) exoY(-)) was more toxic than Pa-T (PAO1F exoS(-) exoY(-)
Formal Description
Interaction-ID: 48854

gene/protein

exoY

NOT decreases_activity of

in primary endothelial cells
Comment ExoS and ExoT, but not by ExoY induce cell retraction in primary endothelial cells derived from veins (HUVEC), capillaries (HMVEC), and arteries (BAEC), a feature associated with disruption of the actin cytoskeleton and focal adhesions. Quantification of cell retraction shows that Pa-S (PAO1F exoT(-) exoY(-)) was more toxic than Pa-T (PAO1F exoS(-) exoY(-)).
Formal Description
Interaction-ID: 48855

gene/protein

exoS

decreases_activity of

in primary endothelial cells
Comment ExoS and ExoT, but not by ExoY induce cell retraction in primary endothelial cells derived from veins (HUVEC), capillaries (HMVEC), and arteries (BAEC), a feature associated with disruption of the actin cytoskeleton and focal adhesions. Quantification of cell retraction shows that Pa-S (PAO1F exoT(-) exoY(-)) was more toxic than Pa-T (PAO1F exoS(-) exoY(-)
Formal Description
Interaction-ID: 48856

gene/protein

exoT

decreases_activity of

in primary endothelial cells
Comment ExoS and ExoT, but not by ExoY induce cell retraction in primary endothelial cells derived from veins (HUVEC), capillaries (HMVEC), and arteries (BAEC), a feature associated with disruption of the actin cytoskeleton and focal adhesions. Quantification of cell retraction shows that Pa-S (PAO1F exoT(-) exoY(-)) was more toxic than Pa-T (PAO1F exoS(-) exoY(-)
Formal Description
Interaction-ID: 48857

gene/protein

exoY

NOT decreases_activity of

in primary endothelial cells
Comment ExoS and ExoT, but not by ExoY induce cell retraction in primary endothelial cells derived from veins (HUVEC), capillaries (HMVEC), and arteries (BAEC), a feature associated with disruption of the actin cytoskeleton and focal adhesions. Quantification of cell retraction shows that Pa-S (PAO1F exoT(-) exoY(-)) was more toxic than Pa-T (PAO1F exoS(-) exoY(-)
Formal Description
Interaction-ID: 48858

gene/protein

exoY

NOT decreases_activity of

in primary endothelial cells
Comment FAK (focal adhesion kinase, PTK2) inactivation was triggered by ExoS and ExoT and paralleled focal adhesion disruption.
Formal Description
Interaction-ID: 48859

gene/protein

exoS

decreases_activity of

gene/protein

PTK2

in primary endothelial cells
Drugbank entries Show/Hide entries for PTK2
Comment ExoS and ExoT affect cofilin and Lim kinase phosphorylation levels. Cofilin is an actin severing and depolymerization factor that is inactivated by phosphorylation. The infection of HUVEC cells with Pa-WT, Pa-S (PAO1F exoT(-) exoY(-)) or Pa-T (PAO1F exoS(-) exoY(-)) induces a dephosphorylation of cofilin.
Formal Description
Interaction-ID: 48861

gene/protein

exoS

decreases_phosphorylation of

gene/protein

CFL1

in primary endothelial cells
Drugbank entries Show/Hide entries for CFL1
Comment ExoS and ExoT affect cofilin and Lim kinase phosphorylation levels. Cofilin is an actin severing and depolymerization factor that is inactivated by phosphorylation. The infection of HUVEC cells with Pa-WT, Pa-S (PAO1F exoT(-) exoY(-)) or Pa-T (PAO1F exoS(-) exoY(-)) induces a dephosphorylation of cofilin.
Formal Description
Interaction-ID: 48862

gene/protein

exoT

decreases_phosphorylation of

gene/protein

CFL1

in primary endothelial cells
Drugbank entries Show/Hide entries for CFL1
Comment FAK (focal adhesion kinase, PTK2) inactivation was triggered by ExoS and ExoT and paralleled focal adhesion disruption.
Formal Description
Interaction-ID: 48864

gene/protein

exoT

decreases_activity of

gene/protein

PTK2

in primary endothelial cells
Drugbank entries Show/Hide entries for PTK2
Comment ExoS and ExoT affect cofilin and Lim kinase phosphorylation levels. Cofilin is an actin severing and depolymerization factor that is inactivated by phosphorylation. The infection of HUVEC cells with Pa-WT, Pa-S (PAO1F exoT(-) exoY(-)) or Pa-T (PAO1F exoS(-) exoY(-)) induces a dephosphorylation of cofilin.
Formal Description
Interaction-ID: 48865

gene/protein

CFL1

affects_activity of

in primary endothelial cells
Drugbank entries Show/Hide entries for CFL1
Comment ExoS and ExoT affect cofilin and Lim kinase phosphorylation levels. The phosphorylation levels of Lim kinase (LIMK1, LIMK2) progressively decreased when HUVECs were infected with Pa-WT, Pa-T (PAO1F exoS(-) exoY(-)), or Pa-S (PAO1F exoT(-) exoY(-)). Lim kinase has a potent role in maintaining the actin cytoskeleton integrity in endothelial cells. .
Formal Description
Interaction-ID: 48866

gene/protein

exoS

decreases_phosphorylation of

gene/protein

LIMK1

in primary endothelial cells
Comment ExoS and ExoT affect cofilin and Lim kinase phosphorylation levels. The phosphorylation levels of Lim kinase (LIMK1, LIMK2) progressively decreased when HUVECs were infected with Pa-WT, Pa-T (PAO1F exoS(-) exoY(-)), or Pa-S (PAO1F exoT(-) exoY(-)). Lim kinase has a potent role in maintaining the actin cytoskeleton integrity in endothelial cells. .
Formal Description
Interaction-ID: 48867

gene/protein

exoS

decreases_phosphorylation of

gene/protein

LIMK2

in primary endothelial cells
Comment ExoS and ExoT affect cofilin and Lim kinase phosphorylation levels. The phosphorylation levels of Lim kinase (LIMK1, LIMK2) progressively decreased when HUVECs were infected with Pa-WT, Pa-T (PAO1F exoS(-) exoY(-)), or Pa-S (PAO1F exoT(-) exoY(-)). Lim kinase has a potent role in maintaining the actin cytoskeleton integrity in endothelial cells. .
Formal Description
Interaction-ID: 48868

gene/protein

exoT

decreases_phosphorylation of

gene/protein

LIMK1

in primary endothelial cells
Comment ExoS and ExoT affect cofilin and Lim kinase phosphorylation levels. The phosphorylation levels of Lim kinase (LIMK1, LIMK2) progressively decreased when HUVECs were infected with Pa-WT, Pa-T (PAO1F exoS(-) exoY(-)), or Pa-S (PAO1F exoT(-) exoY(-)). Lim kinase has a potent role in maintaining the actin cytoskeleton integrity in endothelial cells. .
Formal Description
Interaction-ID: 48869

gene/protein

exoT

decreases_phosphorylation of

gene/protein

LIMK2

in primary endothelial cells
Comment Cofilin activity is downregulated by Lim kinase, which is itself activated by phosphorylation.
Formal Description
Interaction-ID: 48870

gene/protein

LIMK1

decreases_activity of

gene/protein

CFL1

Drugbank entries Show/Hide entries for CFL1
Comment Cofilin activity is downregulated by Lim kinase, which is itself activated by phosphorylation.
Formal Description
Interaction-ID: 48871

gene/protein

LIMK2

decreases_activity of

gene/protein

CFL1

Drugbank entries Show/Hide entries for CFL1
Comment Lim kinase has a potent role in maintaining the actin cytoskeleton integrity in endothelial cells.
Formal Description
Interaction-ID: 48872

gene/protein

LIMK1

increases_activity of

in primary endothelial cells
Comment Lim kinase has a potent role in maintaining the actin cytoskeleton integrity in endothelial cells.
Formal Description
Interaction-ID: 48873

gene/protein

LIMK2

increases_activity of

in primary endothelial cells
Comment ExoT or ExoS individually induced a decrease in RhoA-activity at 3-h post-infection in HUVEC cells.
Formal Description
Interaction-ID: 48874

gene/protein

exoS

decreases_activity of

gene/protein

RHOA

in primary endothelial cells
Drugbank entries Show/Hide entries for RHOA
Comment ExoT or ExoS individually induced a decrease in RhoA-activity at 3-h post-infection in HUVEC cells.
Formal Description
Interaction-ID: 48875

gene/protein

exoT

decreases_activity of

gene/protein

RHOA

in primary endothelial cells
Drugbank entries Show/Hide entries for RHOA
Comment ExoT or ExoS induced a decrease in Rac1-activity at 3-h post-infection in HUVEC cells.
Formal Description
Interaction-ID: 48876

gene/protein

exoS

decreases_activity of

gene/protein

RAC1

in primary endothelial cells
Drugbank entries Show/Hide entries for RAC1
Comment ExoT or ExoS induced a decrease in Rac1-activity at 3-h post-infection in HUVEC cells.
Formal Description
Interaction-ID: 48877

gene/protein

exoT

decreases_activity of

gene/protein

RAC1

in primary endothelial cells
Drugbank entries Show/Hide entries for RAC1
Comment ExoT or ExoS induced a decrease in Cdc42-activity at 3-h post-infection in HUVEC cells.
Formal Description
Interaction-ID: 48878

gene/protein

exoS

decreases_activity of

gene/protein

CDC42

in primary endothelial cells
Drugbank entries Show/Hide entries for CDC42
Comment ExoT or ExoS induced a decrease in Cdc42-activity at 3-h post-infection in HUVEC cells.
Formal Description
Interaction-ID: 48879

gene/protein

exoT

decreases_activity of

gene/protein

CDC42

in primary endothelial cells
Drugbank entries Show/Hide entries for CDC42