General Information:

Id: 4,565
Diseases: Pseudomonas aeruginosa diseases
pathogen-host system
Pseudomonas aeruginosa/mammalia
LPS (E. coli 055:B5) stimulated mouse RAW264.7 macrophage cells, treated with N-3-oxo-dodecanoyl-l-homoserine lactone (C12)
article
Reference: Glucksam-Galnoy Y et al.(2013) The bacterial quorum-sensing signal molecule N-3-oxo-dodecanoyl-L-homoserine lactone reciprocally modulates pro- and anti-inflammatory cytokines in activated macrophages J. Immunol. 191: 337-344 [PMID: 23720811]

Interaction Information:

Comment N-3-oxo-dodecanoyl-l-homoserine lactone (C12) inhibits LPS-induced TNF-alpha secretion in RAW264.7 macrophage cells.
Formal Description
Interaction-ID: 45540

drug/chemical compound

N-(3-Oxododecanoyl)-HSL

decreases_quantity of

gene/protein

TNF

in LPS-stimulated RAW264.7 macrophages
Drugbank entries Show/Hide entries for TNF
Comment N-3-oxo-dodecanoyl-l-homoserine lactone (C12) increases LPS-induced IL-10 secretion in cultured and primary macrophages (peritoneal).
Formal Description
Interaction-ID: 45541

drug/chemical compound

N-(3-Oxododecanoyl)-HSL

increases_quantity of

gene/protein

IL10

in LPS-stimulated RAW264.7 macrophages and peritoneal macrophages
Comment N-3-oxo-dodecanoyl-l-homoserine lactone (C12) increases LPS-induced IL-10 transcription in RAW264.7 macrophages.
Formal Description
Interaction-ID: 45546

drug/chemical compound

N-(3-Oxododecanoyl)-HSL

increases_expression of

gene/protein

IL10

in LPS-stimulated RAW264.7 macrophages
Comment Both N-3-oxo-dodecanoyl-l-homoserine lactone (C12) alone and LPS alone stimulated rapid (15 min) phosphorylation of p38, but only C12 also significantly stimulated p38 phosphorylation over a prolonged time of period of 2 h.
Formal Description
Interaction-ID: 45593

drug/chemical compound

N-(3-Oxododecanoyl)-HSL

increases_phosphorylation of

gene/protein

p38 MAPK

in RAW264.7 macrophages
Comment N-3-oxo-dodecanoyl-l-homoserine lactone (C12) stimulated the phosphorylation of eIF2-alpha in RAW264.7 macrophages at both time points, 15 min and 2 h. This was not observed in cells exposed to LPS alone. The phosphorylation of Ser-51 is known to result in attenuation of protein synthesis.
Formal Description
Interaction-ID: 45594

drug/chemical compound

N-(3-Oxododecanoyl)-HSL

increases_phosphorylation of

gene/protein

EIF2S1

in RAW264.7 macrophages
Comment C12 substantially potentiated LPS-stimulated NF-kappaB DNA-binding levels and prolonged p38 MAPK phosphorylation in RAW264.7 macrophages, suggesting that increased transcriptional activity of NF-kappaB and/or p38-activated transcription factors serves to upregulate IL-10 production in macrophages exposed to both LPS and C12.
Formal Description
Interaction-ID: 45612

drug/chemical compound

N-(3-Oxododecanoyl)-HSL

increases_activity of

in RAW264.7 macrophages