General Information:

Id: 4,475
Diseases: Pseudomonas aeruginosa diseases
pathogen-host system
Pseudomonas aeruginosa/mammalia
Human telomerase-immortalized corneal epithelial cells (hTCEpi), infected with P. aeruginosa strain PAO1
article
Reference: Angus AA et al.(2010) The ADP-ribosylation domain of Pseudomonas aeruginosa ExoS is required for membrane bleb niche formation and bacterial survival within epithelial cells Infect. Immun. 78: 4500-4510 [PMID: 20732998]

Interaction Information:

Comment Mutation of exoS results in loss of bleb niche formation and lack of intracellular replicative capacity. Comparison of mutants that contained multiple-effector deletions revealed that ExoS had the ability to enable bleb niche formation. The bleb niche-forming efficiency of ExoY-expressing PAO1 was low and was not observed for ExoT-expressing PAO1. The ADP-r (ADP-ribosyltransferase) activity of ExoS was responsible for bleb niche-forming capacity and the ability to enable intracellular replication.
Formal Description
Interaction-ID: 45043

gene/protein

exoS

increases_activity of

process

membrane bleb niche formation

in human corneal epithelial cells (hTCEpi)
Comment Mutation of exoS results in loss of bleb niche formation and lack of intracellular replicative capacity. Comparison of mutants that contained multiple-effector deletions revealed that ExoS had the ability to enable bleb niche formation. The bleb niche-forming efficiency of ExoY-expressing PAO1 was low and was not observed for ExoT-expressing PAO1.
Formal Description
Interaction-ID: 45045

gene/protein

exoY

increases_activity of

process

membrane bleb niche formation

in human corneal epithelial cells (hTCEpi); only to a low extend
Comment Mutation of exoS results in loss of bleb niche formation and lack of intracellular replicative capacity. Comparison of mutants that contained multiple-effector deletions revealed that ExoS had the ability to enable bleb niche formation. The bleb niche-forming efficiency of ExoY-expressing PAO1 was low and was not observed for ExoT-expressing PAO1.
Formal Description
Interaction-ID: 45046

gene/protein

exoT

NOT increases_activity of

process

membrane bleb niche formation

in human corneal epithelial cells (hTCEpi)
Comment Mutation of exoS results in loss of bleb niche formation and lack of intracellular replicative capacity. Comparison of mutants that contained multiple-effector deletions revealed that ExoS had the ability to enable bleb niche formation. The bleb niche-forming efficiency of ExoY-expressing PAO1 was low and was not observed for ExoT-expressing PAO1. The ADP-r (ADP-ribosyltransferase) activity of ExoS was responsible for bleb niche-forming capacity and the ability to enable intracellular replication.
Formal Description
Interaction-ID: 45048

gene/protein

exoS

increases_activity of

process

P. aeruginosa intracellular replication

in membrane bleb niches of human corneal epithelial cells (hTCEpi)
Comment Bleb niches display apoptotic markers. ExoS-mediated bleb niches were found to be strongly labeled with the apoptotic marker annexin V-EGFP, which recognizes externalized phosphatidylserine, an early apoptotic cell marker.
Formal Description
Interaction-ID: 45049

process

membrane bleb niche formation

increases_activity of

in human corneal epithelial cells (hTCEpi)