General Information:

Id: 4,283 (click here to show other Interactions for entry)
Diseases: Q fever
pathogen-host system
Coxiella burnetii/mammalia
HeLa cells or J774A.1 cells, infected with substrate mutants
article
Reference: Weber MM et al.(2013) Identification of Coxiella burnetii type IV secretion substrates required for intracellular replication and Coxiella-containing vacuole formation J. Bacteriol. 195: 3914-3924 [PMID: 23813730]

Interaction Information:

Comment The authors showed that 10 T4SS substrate mutants, among 20 mutants analysed, exhibited defects in intracellular growth and CCV formation in HeLa and J774A.1 cells, but displayed normal growth in bacteriological medium. Six substrate mutants (Cbu0041, Cbu0388, Cbu0425, Cbu0937, Cbu2052, and Cbu2059) were unable to replicate in J774A.1 cells or HeLa cells. Due to their role in intracellular replication and CCV formation, Cbu0041, Cbu0425, Cbu0937, Cbu2052, and Cbu2059 were renamed to cirA, cirB, cirC, cirD, and cirE (Coxiella effector for intracellular replication), respectively.
Formal Description
Interaction-ID: 43913

gene/protein

cirA

increases_activity of

process

Coxiella burnetii replication

in HeLa and J774A.1 cells
Drugbank entries Show/Hide entries for cirA
Comment The authors showed that 10 T4SS substrate mutants, among 20 mutants analysed, exhibited defects in intracellular growth and CCV formation in HeLa and J774A.1 cells, but displayed normal growth in bacteriological medium. Six substrate mutants (Cbu0041, Cbu0388, Cbu0425, Cbu0937, Cbu2052, and Cbu2059) were unable to replicate in J774A.1 cells or HeLa cells. Due to their role in intracellular replication and CCV formation, Cbu0041, Cbu0425, Cbu0937, Cbu2052, and Cbu2059 were renamed to cirA, cirB, cirC, cirD, and cirE (Coxiella effector for intracellular replication), respectively.
Formal Description
Interaction-ID: 43917

gene/protein

cirB

increases_activity of

process

Coxiella burnetii replication

in HeLa and J774A.1 cells
Comment The authors showed that 10 T4SS substrate mutants, among 20 mutants analysed, exhibited defects in intracellular growth and CCV formation in HeLa and J774A.1 cells, but displayed normal growth in bacteriological medium. Six substrate mutants (Cbu0041, Cbu0388, Cbu0425, Cbu0937, Cbu2052, and Cbu2059) were unable to replicate in J774A.1 cells or HeLa cells. Due to their role in intracellular replication and CCV formation, Cbu0041, Cbu0425, Cbu0937, Cbu2052, and Cbu2059 were renamed to cirA, cirB, cirC, cirD, and cirE (Coxiella effector for intracellular replication), respectively.
Formal Description
Interaction-ID: 43932

gene/protein

cirC

increases_activity of

process

Coxiella burnetii replication

in HeLa and J774A.1 cells
Comment The authors showed that 10 T4SS substrate mutants, among 20 mutants analysed, exhibited defects in intracellular growth and CCV formation in HeLa and J774A.1 cells, but displayed normal growth in bacteriological medium. Six substrate mutants (Cbu0041, Cbu0388, Cbu0425, Cbu0937, Cbu2052, and Cbu2059) were unable to replicate in J774A.1 cells or HeLa cells. Due to their role in intracellular replication and CCV formation, Cbu0041, Cbu0425, Cbu0937, Cbu2052, and Cbu2059 were renamed to cirA, cirB, cirC, cirD, and cirE (Coxiella effector for intracellular replication), respectively.
Formal Description
Interaction-ID: 43933

gene/protein

cirD

increases_activity of

process

Coxiella burnetii replication

in HeLa and J774A.1 cells
Comment The authors showed that 10 T4SS substrate mutants, among 20 mutants analysed, exhibited defects in intracellular growth and CCV formation in HeLa and J774A.1 cells, but displayed normal growth in bacteriological medium. Six substrate mutants (Cbu0041, Cbu0388, Cbu0425, Cbu0937, Cbu2052, and Cbu2059) were unable to replicate in J774A.1 cells or HeLa cells. Due to their role in intracellular replication and CCV formation, Cbu0041, Cbu0425, Cbu0937, Cbu2052, and Cbu2059 were renamed to cirA, cirB, cirC, cirD, and cirE (Coxiella effector for intracellular replication), respectively.
Formal Description
Interaction-ID: 43935

gene/protein

cirE

increases_activity of

process

Coxiella burnetii replication

in HeLa and J774A.1 cells
Comment The authors showed that 10 T4SS substrate mutants, among 20 mutants analysed, exhibited defects in intracellular growth and CCV formation in HeLa and J774A.1 cells, but displayed normal growth in bacteriological medium. Six substrate mutants (Cbu0041, Cbu0388, Cbu0425, Cbu0937, Cbu2052, and Cbu2059) were unable to replicate in J774A.1 cells or HeLa cells. Due to their role in intracellular replication and CCV formation, Cbu0041, Cbu0425, Cbu0937, Cbu2052, and Cbu2059 were renamed to cirA, cirB, cirC, cirD, and cirE (Coxiella effector for intracellular replication), respectively.
Formal Description
Interaction-ID: 43936

gene/protein

CBU_0388

increases_activity of

process

Coxiella burnetii replication

in HeLa and J774A.1 cells
Comment The authors showed that 10 T4SS substrate mutants, among 20 mutants analysed, exhibited defects in intracellular growth and CCV formation in HeLa and J774A.1 cells, but displayed normal growth in bacteriological medium. Tn insertion into Cbu0012, Cbu1198, Cbu1457, and Cbu2013 resulted in reduced intracellular replication compared to RSA439 levels.
Formal Description
Interaction-ID: 43943

gene/protein

CBU_0012

increases_activity of

process

Coxiella burnetii replication

in J774A.1 cells
Comment The authors showed that 10 T4SS substrate mutants, among 20 mutants analysed, exhibited defects in intracellular growth and CCV formation in HeLa and J774A.1 cells, but displayed normal growth in bacteriological medium. Tn insertion into Cbu0012, Cbu1198, Cbu1457, and Cbu2013 resulted in reduced intracellular replication compared to RSA439 levels.
Formal Description
Interaction-ID: 43944

gene/protein

CBU_1198

increases_activity of

process

Coxiella burnetii replication

in J774A.1 cells
Comment The authors showed that 10 T4SS substrate mutants, among 20 mutants analysed, exhibited defects in intracellular growth and CCV formation in HeLa and J774A.1 cells, but displayed normal growth in bacteriological medium. Tn insertion into Cbu0012, Cbu1198, Cbu1457, and Cbu2013 resulted in reduced intracellular replication compared to RSA439 levels.
Formal Description
Interaction-ID: 43945

gene/protein

CBU_1457

increases_activity of

process

Coxiella burnetii replication

in J774A.1 cells
Comment The authors showed that 10 T4SS substrate mutants, among 20 mutants analysed, exhibited defects in intracellular growth and CCV formation in HeLa and J774A.1 cells, but displayed normal growth in bacteriological medium. Tn insertion into Cbu0012, Cbu1198, Cbu1457, and Cbu2013 resulted in reduced intracellular replication compared to RSA439 levels.
Formal Description
Interaction-ID: 43946

gene/protein

CBU_2013

increases_activity of

process

Coxiella burnetii replication

in J774A.1 cells