General Information:

Id: 4,081
Diseases: Pseudomonas aeruginosa diseases
pathogen-host system
Pseudomonas aeruginosa/mammalia
J774 Eclone macrophages, infected with live or heat-inactivated P. aeruginosa strain PAO1 or PA103
article
Reference: Mustafi S et al.(2013) Regulation of Rab5 Function during Phagocytosis of Live Pseudomonas aeruginosa in Macrophages Infect. Immun. 81: 2426-2436 [PMID: 23630954]

Interaction Information:

Comment Live P. aeruginosa blocked Rab5 activation in macrophages. The authors showed that internalization of live P. aeruginosa strain PAO1 into J774-Eclone macrophages was found to be 75% lower than internalization of heat-inactivated or dead P. aeruginosa after 5, 15, or 30 min of infection.
Formal Description
Interaction-ID: 42078

process

P. aeruginosa infection

decreases_activity of

process

phagocytosis

during the internalization of live (to a larger extend) or heat-inactivated P. aeruginosa
Comment Live P. aeruginosa blocked Rab5 activation in macrophages. The experiments showed that interference of expression of all, but not individual, Rab5 isoforms inhibits phagocytosis of live P. aeruginosa, highlighting the cooperative and redundant regulation of P. aeruginosa phagocytosis by the three Rab5 isoforms (Rab5a,b,c).
Formal Description
Interaction-ID: 42079

process

P. aeruginosa infection

decreases_activity of

gene/protein

RAB5

of Rab5 isoforms a, b, c
Comment Exotoxin S (ExoS) has been shown to play a critical role in Rab5 activation during phagocytosis of P. aeruginosa. The inactivation of Rab5 was dependent on ExoS ADP-ribosyltransferase activity, and in J744-Eclone cells, ExoS ADP-ribosyltransferase activity caused a more severe inhibition of phagocytosis than ExoS Rho GTPase activity.
Formal Description
Interaction-ID: 42099

gene/protein

exoS

decreases_activity of

process

phagocytosis

during the internalization of live (to a larger extend) or heat-inactivated P. aeruginosa
Comment Exotoxin S (ExoS) has been shown to play a critical role in Rab5 activation during phagocytosis of P. aeruginosa. The inactivation of Rab5 was dependent on ExoS ADP-ribosyltransferase activity, and in J744-Eclone cells, ExoS ADP-ribosyltransferase activity caused a more severe inhibition of phagocytosis than ExoS Rho GTPase activity.
Formal Description
Interaction-ID: 42109

gene/protein

exoS

decreases_activity of

gene/protein

RAB5

Comment It has been shown that inhibition of J774-Eclone phagocytosis by ExoS-producing strain PAO1 occurs in conjunction with inhibition of Rab5 activation, while inhibition of phagocytosis by ExoT-producing PA103 strains occurs independently of alterations in Rab5 activation.
Formal Description
Interaction-ID: 42110

gene/protein

exoT

NOT decreases_activity of

gene/protein

RAB5

Comment It has been shown that inhibition of J774-Eclone phagocytosis by ExoS-producing strain PAO1 occurs in conjunction with inhibition of Rab5 activation, while inhibition of phagocytosis by ExoT-producing PA103 strains occurs independently of alterations in Rab5 activation.
Formal Description
Interaction-ID: 42118

gene/protein

exoT

decreases_activity of

process

phagocytosis

during the internalization of live (to a larger extend) or heat-inactivated P. aeruginosa
Comment Rin1, a Rab5 guanine exchange factor, but not Rabex5 and Rap6, partially reversed the inactivation of Rab5 during invasion of live P. aeruginosa. Rin1 expression increased the amount of active GTP-bound Rab5 formed during the internalization of live or heat-inactivated P. aeruginosa, confirming the observation that the expression of Rin1 increased the internalization of live or heat-inactivated P. aeruginosa.
Formal Description
Interaction-ID: 42129

gene/protein

RIN1

increases_activity of

gene/protein

RAB5

during the internalization of live or heat-inactivated P. aeruginosa
Comment Rin1, a Rab5 guanine exchange factor, but not Rabex5 and Rap6, partially reversed the inactivation of Rab5 during invasion of live P. aeruginosa. Rin1 expression increased the amount of active GTP-bound Rab5 formed during the internalization of live or heat-inactivated P. aeruginosa, confirming the observation that the expression of Rin1 increased the internalization of live or heat-inactivated P. aeruginosa.
Formal Description
Interaction-ID: 42132

gene/protein

RIN1

increases_activity of

process

phagocytosis

during the internalization of live or heat-inactivated P. aeruginosa
Comment The authors showed that Rab5 activity was regulated during early stages of P. aeruginosa phagocytosis in J774-Eclone macrophages. Expression of wild-type Rab5 (Rab5:WT) or a Rab5:Q79L, a GTP hydrolysis-defective mutant, increased invasion of heat-inactivated P. aeruginosa, while expression of Rab5:S34N, a GTP-binding-defective mutant, decreased phagocytosis. Rab5 was activated during invasion of heat-inactivated P. aeruginosa but was inactivated during invasion of live P. aeruginosa.
Formal Description
Interaction-ID: 42146

gene/protein

RAB5

increases_activity of

process

phagocytosis