General Information:

Id: 3,961
Diseases: Pseudomonas aeruginosa diseases
pathogen-host system
Pseudomonas aeruginosa/mammalia
human monocytic THP-1 cells, infected with P. aeruginosa strain PA14
article
Reference: Van Alst NE et al.(2009) Nitrite reductase NirS is required for type III secretion system expression and virulence in the human monocyte cell line THP-1 by Pseudomonas aeruginosa Infect. Immun. 77: 4446-4454 [PMID: 19651860]

Interaction Information:

Comment The authors show that both membrane nitrate reductase and nitrite reductase enzyme complexes are important for virulence during the interaction of P. aeruginosa PA14 with THP-1 cells. Deletion mutations in genes encoding membrane nitrate reductase (delta-narGH) and nitrite reductase (delta-nirS) were killed to a significantly greater degree than wild-type PA14 in the THP-1 cell-P. aeruginosa coculture.
Formal Description
Interaction-ID: 40252

complex/PPI

nitrate reductase complex, narGHI

increases_activity of

process

P. aeruginosa infection

Comment The authors show that both membrane nitrate reductase and nitrite reductase enzyme complexes are important for virulence during the interaction of P. aeruginosa PA14 with THP-1 cells. Deletion mutations in genes encoding membrane nitrate reductase (delta-narGH) and nitrite reductase (delta-nirS) were killed to a significantly greater degree than wild-type PA14 in the THP-1 cell-P. aeruginosa coculture.
Formal Description
Interaction-ID: 40254

gene/protein

nirS

increases_activity of

process

P. aeruginosa infection

Drugbank entries Show/Hide entries for nirS
Comment The authors show that both membrane nitrate reductase and nitrite reductase enzyme complexes are important for virulence during the interaction of P. aeruginosa PA14 with THP-1 cells. Deletion mutations in genes encoding membrane nitrate reductase (delta-narGH) and nitrite reductase (delta-nirS) were killed to a significantly greater degree than wild-type PA14 in the THP-1 cell-P. aeruginosa coculture.
Formal Description
Interaction-ID: 40255

gene/protein

narH

is_part_of

complex/PPI

nitrate reductase complex, narGHI

Drugbank entries Show/Hide entries for narH
Comment The authors show that both membrane nitrate reductase and nitrite reductase enzyme complexes are important for virulence during the interaction of P. aeruginosa PA14 with THP-1 cells. Deletion mutations in genes encoding membrane nitrate reductase (delta-narGH) and nitrite reductase (delta-nirS) were killed to a significantly greater degree than wild-type PA14 in the THP-1 cell-P. aeruginosa coculture.
Formal Description
Interaction-ID: 40256

gene/protein

narG

is_part_of

complex/PPI

nitrate reductase complex, narGHI

Drugbank entries Show/Hide entries for narG
Comment The authors show that both membrane nitrate reductase and nitrite reductase enzyme complexes are important for virulence during the interaction of P. aeruginosa PA14 with THP-1 cells. Deletion mutations in genes encoding membrane nitrate reductase (delta-narGH) and nitrite reductase (delta-nirS) were killed to a significantly greater degree than wild-type PA14 in the THP-1 cell-P. aeruginosa coculture.
Formal Description
Interaction-ID: 40257

gene/protein

nirS

increases_quantity of

drug/chemical compound

NO

Drugbank entries Show/Hide entries for nirS
Comment Deletion mutations in genes encoding membrane nitrate reductase (delta-narGH) and nitrite reductase (delta-nirS) display decreased secretion of T3SS exoproducts ExoU, ExoT, and PcrV. The complemented mutants and wild-type PA14 showed comparable degrees of secretion of each T3SS exoproduct.
Formal Description
Interaction-ID: 40258

complex/PPI

nitrate reductase complex, narGHI

increases_activity of

process

T3SS

Comment Deletion mutations in genes encoding membrane nitrate reductase (delta-narGH) and nitrite reductase (delta-nirS) display decreased secretion of T3SS exoproducts ExoU, ExoT, and PcrV. The complemented mutants and wild-type PA14 showed comparable degrees of secretion of each T3SS exoproduct.
Formal Description
Interaction-ID: 40259

gene/protein

nirS

increases_activity of

process

T3SS

Drugbank entries Show/Hide entries for nirS
Comment P. aeruginosa delta-narGH and delta-nirS mutants display decreased total protease and elastase secretion but normal exotoxin A secretion.
Formal Description
Interaction-ID: 40261

complex/PPI

nitrate reductase complex, narGHI

increases_transport of

gene/protein

lasB

Drugbank entries Show/Hide entries for lasB
Comment P. aeruginosa delta-narGH and delta-nirS mutants display decreased total protease and elastase secretion but normal exotoxin A secretion.
Formal Description
Interaction-ID: 40263

gene/protein

nirS

increases_transport of

gene/protein

lasB

Drugbank entries Show/Hide entries for nirS or lasB
Comment P. aeruginosa delta-narGH and delta-nirS mutants display decreased total protease and elastase secretion but normal exotoxin A secretion.
Formal Description
Interaction-ID: 40264

complex/PPI

nitrate reductase complex, narGHI

NOT affects transport of

gene/protein

toxA

Drugbank entries Show/Hide entries for toxA
Comment P. aeruginosa delta-narGH and delta-nirS mutants display decreased total protease and elastase secretion but normal exotoxin A secretion.
Formal Description
Interaction-ID: 40265

gene/protein

nirS

NOT affects transport of

gene/protein

toxA

Drugbank entries Show/Hide entries for nirS or toxA
Comment Addition of exogenous nitric oxide (NO)-generating compounds to delta-nirS mutant cultures restored the production of T3SS phospholipase ExoU, whereas nitrite addition had no effect. These results suggest that NO generated via nitrite reductase NirS contributes to the regulation of expression of selected virulence factors in P. aeruginosa PA14.
Formal Description
Interaction-ID: 40266

drug/chemical compound

NO

increases_activity of

process

T3SS