General Information:

Id: 3,868
Diseases: Pseudomonas aeruginosa diseases
pathogen-host system
Pseudomonas aeruginosa/mammalia
C57BL/6J mice or human lung microvascular endothelial cells (HLMVECs), infected with P. aeruginosa strain PA103
article
Reference: Fu P et al.(2013) Role of nicotinamide adenine dinucleotide phosphate-reduced oxidase proteins in Pseudomonas aeruginosa-induced lung inflammation and permeability Am. J. Respir. Cell Mol. Biol. 48: 477-488 [PMID: 23306835]

Interaction Information:

Comment P. aeruginosa infection of mice induces acute lung injury with increased production of hydrogen peroxide In the lungs. In addition P. aeruginosa induced ROS production in HLMVECs (human lung microvascular endothelial cells) in a dose-dependent manner.
Formal Description
Interaction-ID: 38891

process

P. aeruginosa infection

increases_quantity of

drug/chemical compound

Reactive oxygen species

in the lungs of mice and in HLMVECs
Comment P. aeruginosa lung infection induces NOX2 (CYBB) and NOX4 expression, but not, NOX1 and NOX3. Immunohistochemistry staining of lung tissues showed an increase in expression of NOX2 and NOX4 proteins, but not NOX-1 or NOX3. NOX2 was detectable in neutrophils, lung endothelial and epithelial cells whereas the expression of NOX4 was seen mainly in lung endothelial and epithelial cells. RT-PCR only showed increase in NOX2 mRNA and NOX4 mRNA.
Formal Description
Interaction-ID: 38892

process

P. aeruginosa infection

increases_quantity of

gene/protein

CYBB

in the lungs of mice and in HLMVECs
Comment P. aeruginosa lung infection induces NOX2 (CYBB) and NOX4 expression, but not, NOX1 and NOX3. Immunohistochemistry staining of lung tissues showed an increase in expression of NOX2 and NOX4 proteins, but not NOX-1 or NOX3. NOX2 was detectable in neutrophils, lung endothelial and epithelial cells whereas the expression of NOX4 was seen mainly in lung endothelial and epithelial cells. RT-PCR only showed increase in NOX2 mRNA and NOX4 mRNA.
Formal Description
Interaction-ID: 38893

process

P. aeruginosa infection

increases_expression of

gene/protein

CYBB

in the lungs of mice and in HLMVECs
Comment P. aeruginosa lung infection induces NOX2 (CYBB) and NOX4 expression, but not, NOX1 and NOX3. Immunohistochemistry staining of lung tissues showed an increase in expression of NOX2 and NOX4 proteins, but not NOX-1 or NOX3. NOX2 was detectable in neutrophils, lung endothelial and epithelial cells whereas the expression of NOX4 was seen mainly in lung endothelial and epithelial cells. RT-PCR only showed increase in NOX2 mRNA and NOX4 mRNA.
Formal Description
Interaction-ID: 38894

process

P. aeruginosa infection

increases_expression of

gene/protein

NOX4

in the lungs of mice and in HLMVECs
Comment P. aeruginosa lung infection induces NOX2 (CYBB) and NOX4 expression, but not, NOX1 and NOX3. Immunohistochemistry staining of lung tissues showed an increase in expression of NOX2 and NOX4 proteins, but not NOX-1 or NOX3. NOX2 was detectable in neutrophils, lung endothelial and epithelial cells whereas the expression of NOX4 was seen mainly in lung endothelial and epithelial cells. RT-PCR only showed increase in NOX2 mRNA and NOX4 mRNA.
Formal Description
Interaction-ID: 38895

process

P. aeruginosa infection

increases_quantity of

gene/protein

NOX4

in the lungs of mice and in HLMVECs
Comment P. aeruginosa lung infection induces NOX2 (CYBB) and NOX4 expression, but not, NOX1 and NOX3. Immunohistochemistry staining of lung tissues showed an increase in expression of NOX2 and NOX4 proteins, but not NOX-1 or NOX3. NOX2 was detectable in neutrophils, lung endothelial and epithelial cells whereas the expression of NOX4 was seen mainly in lung endothelial and epithelial cells. RT-PCR only showed increase in NOX2 mRNA and NOX4 mRNA.
Formal Description
Interaction-ID: 38896

process

P. aeruginosa infection

NOT increases_quantity of

gene/protein

NOX1

in the lungs of mice and in HLMVECs
Comment P. aeruginosa lung infection induces NOX2 (CYBB) and NOX4 expression, but not, NOX1 and NOX3. Immunohistochemistry staining of lung tissues showed an increase in expression of NOX2 and NOX4 proteins, but not NOX-1 or NOX3. NOX2 was detectable in neutrophils, lung endothelial and epithelial cells whereas the expression of NOX4 was seen mainly in lung endothelial and epithelial cells. RT-PCR only showed increase in NOX2 mRNA and NOX4 mRNA.
Formal Description
Interaction-ID: 38897

process

P. aeruginosa infection

NOT increases_quantity of

gene/protein

NOX3

in the lungs of mice and in HLMVECs
Comment P. aeruginosa infection activates NF-kappa-B pathway both in vivo in mouse lung and in vitro in endothelial cells. In addition it has been shown that P. aeruginosa-induced expression of NOX2 (CYBB) and NOX4 proteins in endothelial cells or in the lungs is mediated by the NF-kappa-B signaling pathway.
Formal Description
Interaction-ID: 38898

process

P. aeruginosa infection

increases_activity of

in the lungs of mice and in HLMVECs
Comment P. aeruginosa infection activates NF-kappa-B pathway both in vivo in mouse lung and in vitro in endothelial cells. In addition it has been shown that P. aeruginosa-induced expression of NOX2 (CYBB) and NOX4 proteins in endothelial cells or in the lungs is mediated by the NF-kappa-B signaling pathway.
Formal Description
Interaction-ID: 38899

increases_expression of

gene/protein

CYBB

in the lungs of mice and in HLMVECs
Comment P. aeruginosa infection activates NF-kappa-B pathway both in vivo in mouse lung and in vitro in endothelial cells. In addition it has been shown that P. aeruginosa-induced expression of NOX2 and NOX4 proteins in endothelial cells or in the lungs is mediated by the NF-kappa-B signaling pathway.
Formal Description
Interaction-ID: 38900

increases_expression of

gene/protein

NOX4

in the lungs of mice and in HLMVECs
Comment P. aeruginosa significantly induced endothelial barrier permeability in a dose-dependent manner.
Formal Description
Interaction-ID: 38906

process

P. aeruginosa infection

decreases_activity of

Comment Knockdown experiments showed that there is an important role for NOX4, but not NOX2 (CYBB), in mediating the loss of endothelial barrier integrity due to P.aeruginosa infection.
Formal Description
Interaction-ID: 38907

gene/protein

NOX4

decreases_activity of

as shown in HLMVECs
Comment Knockdown experiments showed that there is an important role for NOX4, but not NOX2 (CYBB), in mediating the loss of endothelial barrier integrity due to P.aeruginosa infection.
Formal Description
Interaction-ID: 38908

gene/protein

CYBB

NOT decreases_activity of

as shown in HLMVECs
Comment P. aeruginosa induced significant disruption of VE-cadherin and ZO-1 from cell adhesion junction, and stress fiber formation, which was attenuated by NOX4 siRNA.
Formal Description
Interaction-ID: 38909

process

P. aeruginosa infection

decreases_activity of

as shown in HLMVECs
Comment P. aeruginosa induced significant disruption of VE-cadherin and ZO-1 from cell adhesion junction, and stress fiber formation, which was attenuated by NOX4 siRNA.
Formal Description
Interaction-ID: 38917

process

P. aeruginosa infection

increases_activity of

as shown in HLMVECs
Comment P. aeruginosa induced significant disruption of VE-cadherin and ZO-1 from cell adhesion junction, and stress fiber formation, which was attenuated by NOX4 siRNA.
Formal Description
Interaction-ID: 38918

gene/protein

NOX4

decreases_activity of

as shown in HLMVECs
Comment P. aeruginosa induced significant disruption of VE-cadherin and ZO-1 from cell adhesion junction, and stress fiber formation, which was attenuated by NOX4 siRNA.
Formal Description
Interaction-ID: 38919

gene/protein

NOX4

increases_activity of

as shown in HLMVECs
Comment Knockdown of NOX4 blocks P. aeruginosa induced-apoptosis In HLMVECs.
Formal Description
Interaction-ID: 38921

process

P. aeruginosa infection

increases_activity of

as shown in HLMVECs
Comment Knockdown of NOX4 blocks P. aeruginosa induced-apoptosis In HLMVECs.
Formal Description
Interaction-ID: 38922

gene/protein

NOX4

increases_activity of

as shown in HLMVECs
Comment Genetic knockdown of NOX2 (CYBB) (wild type mouse model compared with NOX2(-/-) mice) attenuates inflammatory response to P. aeruginosa but not the permeability. In wild type PBS instilled control mice, P. aeruginosa induced significant increase in IL-6, TNF-alpha and H2O2 in BAL (bronchoalveolar lavage) fluids, in NOX2(-/-) mice, P. aeruginosa induced significantly lower levels of IL-6, TNF-alpha; and H2O2.
Formal Description
Interaction-ID: 38924

process

P. aeruginosa infection

increases_quantity of

gene/protein

IL6

in BAL fluids of mice
Drugbank entries Show/Hide entries for IL6
Comment Genetic knockdown of NOX2 (CYBB) (wild type mouse model compared with NOX2(-/-) mice) attenuates inflammatory response to P. aeruginosa but not the permeability. In wild type PBS instilled control mice, P. aeruginosa induced significant increase in IL-6, TNF-alpha and H2O2 in BAL (bronchoalveolar lavage) fluids, in NOX2(-/-) mice, P. aeruginosa induced significantly lower levels of IL-6, TNF-alpha; and H2O2.
Formal Description
Interaction-ID: 38927

process

P. aeruginosa infection

increases_quantity of

gene/protein

TNF

in BAL fluids of mice
Drugbank entries Show/Hide entries for TNF
Comment Genetic knockdown of NOX2 (CYBB) (wild type mouse model compared with NOX2(-/-) mice) attenuates inflammatory response to P. aeruginosa but not the permeability. In wild type PBS instilled control mice, P. aeruginosa induced significant increase in IL-6, TNF-alpha and H2O2 in BAL (bronchoalveolar lavage) fluids, in NOX2(-/-) mice, P. aeruginosa induced significantly lower levels of IL-6, TNF-alpha; and H2O2.
Formal Description
Interaction-ID: 38928

gene/protein

CYBB

increases_quantity of

gene/protein

IL6

in BAL fluids of mice
Drugbank entries Show/Hide entries for IL6
Comment Genetic knockdown of NOX2 (CYBB) (wild type mouse model compared with NOX2(-/-) mice) attenuates inflammatory response to P. aeruginosa but not the permeability. In wild type PBS instilled control mice, P. aeruginosa induced significant increase in IL-6, TNF-alpha and H2O2 in BAL (bronchoalveolar lavage) fluids, in NOX2(-/-) mice, P. aeruginosa induced significantly lower levels of IL-6, TNF-alpha; and H2O2.
Formal Description
Interaction-ID: 38929

gene/protein

CYBB

increases_quantity of

gene/protein

TNF

in BAL fluids of mice
Drugbank entries Show/Hide entries for TNF
Comment Genetic knockdown of NOX2 (CYBB) (wild type mouse model compared with NOX2(-/-) mice) attenuates inflammatory response to P. aeruginosa but not the permeability. In wild type PBS instilled control mice, P. aeruginosa induced significant increase in IL-6, TNF-alpha and H2O2 in BAL (bronchoalveolar lavage) fluids, in NOX2(-/-) mice, P. aeruginosa induced significantly lower levels of IL-6, TNF-alpha; and H2O2.
Formal Description
Interaction-ID: 38930

gene/protein

CYBB

increases_quantity of

drug/chemical compound

Reactive oxygen species

in BAL fluids of mice
Comment Genetic knockdown of NOX2 (CYBB) (wild type mouse model compared with NOX2(-/-) mice) attenuates inflammatory response to P. aeruginosa but not the permeability. In wild type PBS instilled control mice, P. aeruginosa induced significant increase in IL-6, TNF-alpha and H2O2 in BAL (bronchoalveolar lavage) fluids, in NOX2(-/-) mice, P. aeruginosa induced significantly lower levels of IL-6, TNF-alpha; and H2O2.
Formal Description
Interaction-ID: 38938

gene/protein

CYBB

NOT increases_activity of

process

pulmonary permeability

Comment Unlike NOX2(-/-) (CYBB-/-) mice, knockdown of NOX4 inhibited P. aeruginosa-induced pulmonary permeability as evidenced by BAL (bronchoalveolar lavage) fluids cell count and protein concentration. On the contrary, knockdown of NOX4 had no effects on IL-6, TNF-alpha and H2O2 in BAL fluids.
Formal Description
Interaction-ID: 38939

gene/protein

NOX4

increases_activity of

process

pulmonary permeability

Comment Unlike NOX2(-/-) (CYBB-/-) mice, knockdown of NOX4 inhibited P. aeruginosa-induced pulmonary permeability as evidenced by BAL (bronchoalveolar lavage) fluids cell count and protein concentration. On the contrary, knockdown of NOX4 had no effects on IL-6, TNF-alpha and H2O2 in BAL fluids.
Formal Description
Interaction-ID: 38940

gene/protein

NOX4

NOT affects_quantity of

gene/protein

IL6

in BAL fluids of mice
Drugbank entries Show/Hide entries for IL6
Comment Unlike NOX2(-/-) (CYBB-/-) mice, knockdown of NOX4 inhibited P. aeruginosa-induced pulmonary permeability as evidenced by BAL (bronchoalveolar lavage) fluids cell count and protein concentration. On the contrary, knockdown of NOX4 had no effects on IL-6, TNF-alpha and H2O2 in BAL fluids.
Formal Description
Interaction-ID: 38941

gene/protein

NOX4

NOT affects_quantity of

gene/protein

TNF

in BAL fluids of mice
Drugbank entries Show/Hide entries for TNF
Comment Unlike NOX2(-/-) (CYBB-/-) mice, knockdown of NOX4 inhibited P. aeruginosa-induced pulmonary permeability as evidenced by BAL (bronchoalveolar lavage) fluids cell count and protein concentration. On the contrary, knockdown of NOX4 had no effects on IL-6, TNF-alpha and H2O2 in BAL fluids.
Formal Description
Interaction-ID: 38942

gene/protein

NOX4

NOT affects_quantity of

drug/chemical compound

Reactive oxygen species

in BAL fluids of mice
Comment Genetic knockdown of NOX2 (CYBB) In mice attenuates inflammatory response to P. aeruginosa but not the permeability.
Formal Description
Interaction-ID: 38944

process

P. aeruginosa infection

increases_activity of

Comment Genetic knockdown of NOX2 (CYBB) In mice attenuates inflammatory response to P. aeruginosa but not the permeability.
Formal Description
Interaction-ID: 38945

gene/protein

CYBB

increases_activity of

Comment Unlike NOX2(-/-) (CYBB-/-) mice, knockdown of NOX4 inhibited P. aeruginosa-induced pulmonary permeability as evidenced by BAL (bronchoalveolar lavage) fluids cell count and protein concentration. On the contrary, knockdown of NOX4 had no effects on IL-6, TNF-alpha and H2O2 in BAL fluids.
Formal Description
Interaction-ID: 38946

gene/protein

NOX4

NOT affects_activity of