General Information:

Id: 3,838
Diseases: Pseudomonas aeruginosa diseases
pathogen-host system
Pseudomonas aeruginosa/mammalia
foreskin-derived primary keratinocytes, infected with supernatants from P. aeruginosa CF391 grown in PG (1% peptone-1% glucose) medium or LB (lysogeny broth) medium
article
Reference: Dossel J et al.(2012) Pseudomonas aeruginosa-derived rhamnolipids subvert the host innate immune response through manipulation of the human beta-defensin-2 expression Cell. Microbiol. 14: 1364-1375 [PMID: 22500651]

Interaction Information:

Comment P. aeruginosa strain CF391-derived culture supernatants cause opposite effects on hBD-2 release in human keratinocytes. LB (lysogeny broth medium grown P. aeruginosa) supernatants induced hBD-2 release in cultured keratinocytes comparable to purified P. aeruginosa-derived flagellin, whereas supernatants of P. aeruginosa grown in PG medium (1% peptone-1% glucose) did not induce hBD-2.
Formal Description
Interaction-ID: 38519

gene/protein

fliC

increases_quantity of

gene/protein

DEFB4A

Comment Rhamnolipids are the PG-derived (P. aeruginosa grown in 1% peptone-1% glucose medium) factors inhibiting the flagellin-induced hBD-2 (DEFB4A) response. Rhamnolipids inhibited flagellin-induced hBD-2 secretion and mRNA induction.
Formal Description
Interaction-ID: 38521

cellular component

rhamnolipid

decreases_quantity of

gene/protein

DEFB4A

in keratinocytes, treated with rhamnolipids
Comment Rhamnolipids are the PG-derived (P. aeruginosa grown in 1% peptone-1% glucose medium) factors inhibiting the flagellin-induced hBD-2 (DEFB4A) response. Rhamnolipids inhibited flagellin-induced hBD-2 secretion and mRNA induction.
Formal Description
Interaction-ID: 38522

cellular component

rhamnolipid

decreases_expression of

gene/protein

DEFB4A

in keratinocytes, treated with rhamnolipids
Comment P. aeruginosa strain CF391-derived culture supernatants cause opposite effects on hBD-2 (DEFB4A) release in human keratinocytes. LB (lysogeny broth medium grown P. aeruginosa) supernatants induced hBD-2 release in cultured keratinocytes comparable to purified P. aeruginosa-derived flagellin, whereas supernatants of P. aeruginosa grown in PG medium (1% peptone-1% glucose) did not induce hBD-2.
Formal Description
Interaction-ID: 38556

process

P. aeruginosa infection

increases_quantity of

gene/protein

DEFB4A

in keratinocytes, infected with supernatants from P. aeruginosa CF391 grown in LB (lysogeny broth) medium
Comment P. aeruginosa strain CF391-derived culture supernatants cause opposite effects on hBD-2 (DEFB4A) release in human keratinocytes. LB (lysogeny broth medium grown P. aeruginosa) supernatants induced hBD-2 release in cultured keratinocytes comparable to purified P. aeruginosa-derived flagellin, whereas supernatants of P. aeruginosa grown in PG medium (1% peptone-1% glucose) did not induce hBD-2.
Formal Description
Interaction-ID: 38558

process

P. aeruginosa infection

NOT increases_quantity of

gene/protein

DEFB4A

in keratinocytes, infected with supernatants from P. aeruginosa CF391 grown in PG (1% peptone-1% glucose) medium
Comment Rhamnolipids are the PG-derived (P. aeruginosa grown in 1% peptone-1% glucose medium) factors inhibiting the flagellin-induced hBD-2 (DEFB4A) response. Rhamnolipids inhibited flagellin-induced hBD-2 secretion and mRNA induction.
Formal Description
Interaction-ID: 38563

cellular component

rhamnolipid

decreases_activity of

gene/protein

fliC

in keratinocytes, treated with rhamnolipids
Comment Rhamnolipids manipulate the flagellin- and IL-1-beta induced hBD-2 release but not the flagellin-induced IL-8 release in keratinocytes. IL-1-beta is a potent inducer of hBD-2 expression in keratinocytes. Addition of rhamnolipids significantly reduced the induction of hBD-2 by IL-1-beta to basal levels.
Formal Description
Interaction-ID: 38564

gene/protein

IL1B

increases_quantity of

gene/protein

DEFB4A

in keratinocytes
Drugbank entries Show/Hide entries for IL1B
Comment Rhamnolipids manipulate the flagellin- and IL-1-beta induced hBD-2 release but not the flagellin-induced IL-8 release in keratinocytes. IL-1-beta is a potent inducer of hBD-2 expression in keratinocytes. Addition of rhamnolipids significantly reduced the induction of hBD-2 by IL-1-beta to basal levels.
Formal Description
Interaction-ID: 38565

cellular component

rhamnolipid

decreases_activity of

gene/protein

IL1B

in keratinocytes
Drugbank entries Show/Hide entries for IL1B
Comment Rhamnolipids manipulate the flagellin- and IL-1-beta induced hBD-2 release but not the flagellin-induced IL-8 release in keratinocytes. Flagellin induced the release of IL-8 in keratinocytes, but in contrast to hBD-2, co-incubation of rhamnolipids did not diminish the flagellin-induced IL-8 release significantly.
Formal Description
Interaction-ID: 38566

gene/protein

fliC

increases_quantity of

gene/protein

CXCL8

in keratinocytes
Comment Rhamnolipids manipulate the flagellin- and IL-1-beta induced hBD-2 release but not the flagellin-induced IL-8 release in keratinocytes. Flagellin induced the release of IL-8 in keratinocytes, but in contrast to hBD-2, co-incubation of rhamnolipids did not diminish the flagellin-induced IL-8 release significantly.
Formal Description
Interaction-ID: 38567

cellular component

rhamnolipid

NOT affects_quantity of

gene/protein

CXCL8

in keratinocytes
Comment Rhamnolipids affect calcium signalling in keratinocytes thereby targeting PKC. PMA (Phorbol 12-myristate 13-acetate) a commonly used phorbol ester that activates calcium-dependent PKC, is known to induce hBD-2. Preincubation with either GOE6979, an inhibitor of PKC, or rhamnolipids significantly reduced PMA-mediated hBD-2 release in keratinocytes.
Formal Description
Interaction-ID: 38573

increases_activity of

gene/protein

Protein kinase C

Comment Rhamnolipids affect calcium signalling in keratinocytes thereby targeting PKC. PMA (Phorbol 12-myristate 13-acetate) a commonly used phorbol ester that activates calcium-dependent PKC, is known to induce hBD-2. Preincubation with either GOE6979, an inhibitor of PKC, or rhamnolipids significantly reduced PMA-mediated hBD-2 release in keratinocytes.
Formal Description
Interaction-ID: 38582

gene/protein

Protein kinase C

increases_quantity of

gene/protein

DEFB4A

in keratinocytes
Comment Rhamnolipids affect calcium signalling in keratinocytes thereby targeting PKC. PMA (Phorbol 12-myristate 13-acetate) a commonly used phorbol ester that activates calcium-dependent PKC, is known to induce hBD-2. Preincubation with either GOE6979, an inhibitor of PKC, or rhamnolipids significantly reduced PMA-mediated hBD-2 release in keratinocytes.
Formal Description
Interaction-ID: 38609

drug/chemical compound

Ca2+

affects_activity of

gene/protein

Protein kinase C

in keratinocytes