General Information:

Id: 3,810 (click here to show other Interactions for entry)
Diseases: Q fever
pathogen-host system
Coxiella burnetii/mammalia
HeLa cells, siRNA treated, infected with C. burnetii NMII, producing BlaM-77 fusion protein
article
Reference: McDonough JA et al.(2013) Host pathways important for Coxiella burnetii infection revealed by genome-wide RNA interference screening MBio 4 [PMID: 23362322]

Interaction Information:

Comment Depletion of VPS35 levels by gene silencing resulted in a defect in BlaM-77 translocation, showing that the retromer pathway is important for delivery of C. burnetii effectors.
Formal Description
Interaction-ID: 38078

complex/PPI

Retromer complex

increases_activity of

process

T4BSS

Comment Depletion of VPS35 levels by gene silencing resulted in a defect in BlaM-77 translocation, showing that the retromer pathway is important for delivery of C. burnetii effectors.
Formal Description
Interaction-ID: 38095

gene/protein

VPS35

increases_activity of

process

T4BSS

Comment Silencing of STX17 had no effect on translocation of BlaM-77.
Formal Description
Interaction-ID: 38098

gene/protein

STX17

NOT affects_activity of

process

T4BSS

Comment Although VPS16 was also found to be required for CCV formation, there was no translocation defect for BlaM-77, BlaM-1823 and BlaM-1524 in cells where VPS16 had been silenced.
Formal Description
Interaction-ID: 38099

gene/protein

VPS16

NOT affects_activity of

process

T4BSS

Comment Depletion of RAB7A showed a reduced effector translocation for BlaM-77, BlaM-1823 and BlaM-1524 (caeA).
Formal Description
Interaction-ID: 38100

gene/protein

RAB7A

increases_activity of

process

T4BSS

Drugbank entries Show/Hide entries for RAB7A
Comment The depletion of the retromer associated sorting nexins SNX2, SNX3, SNX5, SNX6 resulted in reduced BlaM-77 translocation. This shows that the retromer and most associated sorting nexins play important roles in stages of CCV maturation that are important for effector translocation.
Formal Description
Interaction-ID: 38161

gene/protein

SNX2

increases_activity of

process

T4BSS

in C. burnetii infected HeLa cells; analysed by treatment with SNX2 siRNA and the BlaM translocation assay
Comment The depletion of the retromer associated sorting nexins SNX2, SNX3, SNX5, SNX6 resulted in reduced BlaM-77 translocation. This shows that the retromer and most associated sorting nexins play important roles in stages of CCV maturation that are important for effector translocation.
Formal Description
Interaction-ID: 38162

gene/protein

SNX3

increases_activity of

process

T4BSS

in C. burnetii infected HeLa cells; analysed by treatment with SNX3 siRNA and the BlaM translocation assay
Drugbank entries Show/Hide entries for SNX3
Comment The depletion of the retromer associated sorting nexins SNX2, SNX3, SNX5, SNX6 resulted in reduced BlaM-77 translocation. This shows that the retromer and most associated sorting nexins play important roles in stages of CCV maturation that are important for effector translocation.
Formal Description
Interaction-ID: 38163

gene/protein

SNX5

increases_activity of

process

T4BSS

in C. burnetii infected HeLa cells; analysed by treatment with SNX5 siRNA and the BlaM translocation assay
Comment The depletion of the retromer associated sorting nexins SNX2, SNX3, SNX5, SNX6 resulted in reduced BlaM-77 translocation. This shows that the retromer and most associated sorting nexins play important roles in stages of CCV maturation that are important for effector translocation.
Formal Description
Interaction-ID: 38164

gene/protein

SNX6

increases_activity of

process

T4BSS

in C. burnetii infected HeLa cells; analysed by treatment with SNX6 siRNA and the BlaM translocation assay