General Information:

Id: 3,806 (click here to show other Interactions for entry)
Diseases: Q fever
pathogen-host system
Coxiella burnetii/mammalia
C57BL/6 mice derived bone marrow macrophages (BMMs), infected with C. burnetii NMII producing BlaM-effector fusion proteins BlaM alone, BlaM-77, BlaM-1823, BlaM-1524
article
Reference: Newton HJ et al.(2013) Effector Protein Translocation by the Coxiella burnetii Dot/Icm Type IV Secretion System Requires Endocytic Maturation of the Pathogen-Occupied Vacuole PLoS ONE 8 [PMID: 23349930]

Interaction Information:

Comment As shown in HeLa cells, assays in BMMs (bone marrow derived macrophages) determined that the three different BlaM-effector fusions (CBU0077, CBU1823 and CBU1524 (caeA)) were translocated during infection by C. burnetii. In BMMs there were a small number of translocation-positive cells detected at four hours post-infection for all three effector proteins, which suggests that the Dot/Icm system is activated sooner following uptake by BMMs.
Formal Description
Interaction-ID: 37936

process

T4BSS

increases_transport of

gene/protein

CBU_0077

into BMMs; detected at four hours post-infection, shown in a BlaM translocation assay
Comment As shown in HeLa cells, assays in BMMs (bone marrow derived macrophages) determined that the three different BlaM-effector fusions (CBU0077, CBU1823 and CBU1524 (caeA)) were translocated during infection by C. burnetii. In BMMs there were a small number of translocation-positive cells detected at four hours post-infection for all three effector proteins, which suggests that the Dot/Icm system is activated sooner following uptake by BMMs.
Formal Description
Interaction-ID: 37937

process

T4BSS

increases_transport of

gene/protein

caeA

into BMMs; detected at four hours post-infection, shown in a BlaM translocation assay
Comment As shown in HeLa cells, assays in BMMs (bone marrow derived macrophages) determined that the three different BlaM-effector fusions (CBU0077, CBU1823 and CBU1524 (caeA)) were translocated during infection by C. burnetii. In BMMs there were a small number of translocation-positive cells detected at four hours post-infection for all three effector proteins, which suggests that the Dot/Icm system is activated sooner following uptake by BMMs.
Formal Description
Interaction-ID: 37938

process

T4BSS

increases_transport of

gene/protein

CBU_1823

into BMMs; detected at four hours post-infection, shown in a BlaM translocation assay
Comment Effector translocation by C. burnetii was efficiently blocked when the pH of intracellular organelles was neutralized by treating BMMs with BafA (Bafilomycin A) during infection.
Formal Description
Interaction-ID: 37941

increases_activity of

process

T4BSS

in C. burnetii infected BMMs