General Information:

Id: 3,805 (click here to show other Interactions for entry)
Diseases: Q fever
pathogen-host system
Coxiella burnetii/mammalia
HeLa cells, infected with C. burnetii NMII, producing BlaM-effector fusion proteins BlaM alone, BlaM-77, BlaM-1823, BlaM-1524
article
Reference: Newton HJ et al.(2013) Effector Protein Translocation by the Coxiella burnetii Dot/Icm Type IV Secretion System Requires Endocytic Maturation of the Pathogen-Occupied Vacuole PLoS ONE 8 [PMID: 23349930]

Interaction Information:

Comment Translocation of BlaM (reporter enzyme beta-lactamase) fused to the effector proteins CBU0077, CBU1823 and CBU1524 (caeA) of C. burnetii was not detected until 8-hours after infection of HeLa cells. This delay seems to be representative for the delivery of effectors by the Dot/Icm system of C. burnetii.
Formal Description
Interaction-ID: 37850

process

T4BSS

increases_transport of

gene/protein

CBU_0077

into HeLa cells; not detected before 8 hours post-infection, shown in a BlaM translocation assay
Comment Translocation of BlaM (reporter enzyme beta-lactamase) fused to the effector proteins CBU0077, CBU1823 and CBU1524 (caeA) of C. burnetii was not detected until 8-hours after infection of HeLa cells. This delay seems to be representative for the delivery of effectors by the Dot/Icm system of C. burnetii.
Formal Description
Interaction-ID: 37868

process

T4BSS

increases_transport of

gene/protein

caeA

into HeLa cells; not detected before 8 hours post-infection, shown in a BlaM translocation assay
Comment Translocation of BlaM (reporter enzyme beta-lactamase) fused to the effector proteins CBU0077, CBU1823 and CBU1524 (caeA) of C. burnetii was not detected until 8-hours after infection of HeLa cells. This delay seems to be representative for the delivery of effectors by the Dot/Icm system of C. burnetii.
Formal Description
Interaction-ID: 37870

process

T4BSS

increases_transport of

gene/protein

CBU_1823

into HeLa cells; not detected before 8 hours post-infection, shown in a BlaM translocation assay
Comment Acidification of the Coxiella containing vacuole, CCV is required for Dot/Icm dependent effector translocation. Blocking the acidification of intracellular organelles with Bafilomycin A (BafA; 100 nM), a specific inhibitor of the vacuolar ATPase, results in no detectable translocation of any fusion protein detected after cells were infected for 24 hours.
Formal Description
Interaction-ID: 37877

increases_activity of

process

T4BSS

in C. burnetii infected HeLa cells
Comment Translocation efficiencies for all three effector fusion proteins (CBU0077, CBU1823 and CBU1524 (caeA)) dropped significantly in cells where Rab5 or Rab7 had been silenced. Rab5 is known to interfere with early endosomal maturation events, Rab7 with late endosomal maturation events.
Formal Description
Interaction-ID: 37898

gene/protein

RAB5

increases_activity of

process

T4BSS

in C. burnetii infected HeLa cells
Comment Translocation efficiencies for all three effector fusion proteins (CBU0077, CBU1823 and CBU1524 (caeA)) dropped significantly in cells where Rab5 or Rab7 had been silenced. Rab5 is known to interfere with early endosomal maturation events, Rab7 with late endosomal maturation events.
Formal Description
Interaction-ID: 37899

gene/protein

RAB7

increases_activity of

process

T4BSS

in C. burnetii infected HeLa cells
Comment Translocation efficiencies were not affected similarly in control cells where Rab1 was silenced. Rab1 is known to interfere with the transport of early secretory vesicles.
Formal Description
Interaction-ID: 37900

gene/protein

RAB1

NOT increases_activity of

process

T4BSS

in C. burnetii infected HeLa cells
Comment The results show that maturation of the Coxiella containing vacuole, CCV to a late-endocytic compartment through the activities of Rab5 and Rab7 stimulate delivery of effectors by the Dot/Icm system.
Formal Description
Interaction-ID: 37909

increases_activity of

process

T4BSS

in C. burnetii infected HeLa cells