General Information:

Id: 3,774
Diseases: Q fever
pathogen-host system
Coxiella burnetii/mammalia
THP-1 cells or Vero cells, infected with phase I or phase II C. burnetii
article
Reference: Voth DE and Heinzen RA(2009) Sustained activation of Akt and Erk1/2 is required for Coxiella burnetii antiapoptotic activity Infect. Immun. 77: 205-213 [PMID: 18981248]

Interaction Information:

Comment The infection of THP-1 cells with C. burnetii phase II caused transient phosphorylation of c-Jun, Hsp27, JNK, and p38. The phosphorylation levels dramatically increased at 2 hpi, followed by a decline to near basal levels by 24 hpi, as shown in a Bio-Plex assay and immunoblot analysis.
Formal Description
Interaction-ID: 36867

organism

Coxiella burnetii, phase II

increases_activity of

gene/protein

JUN

in THP-1 cells; at 2 h postinfection (hpi)
Drugbank entries Show/Hide entries for JUN
Comment The infection of THP-1 cells with C. burnetii phase II caused transient phosphorylation of c-Jun, Hsp27, JNK, and p38. The phosphorylation levels dramatically increased at 2 hpi, followed by a decline to near basal levels by 24 hpi.
Formal Description
Interaction-ID: 36868

organism

Coxiella burnetii, phase II

increases_phosphorylation of

gene/protein

JUN

in THP-1 cells; at 2 h postinfection (hpi)
Drugbank entries Show/Hide entries for JUN
Comment The infection of THP-1 cells with C. burnetii phase II caused transient phosphorylation of c-Jun, Hsp27, JNK, and p38. The phosphorylation levels dramatically increased at 2 hpi, followed by a decline to near basal levels by 24 hpi, as shown in a Bio-Plex assay and immunoblot analysis.
Formal Description
Interaction-ID: 36869

organism

Coxiella burnetii, phase II

increases_activity of

gene/protein

HSPB1

in THP-1 cells; at 2 h postinfection (hpi)
Comment The infection of THP-1 cells with C. burnetii phase II caused transient phosphorylation of c-Jun, Hsp27, JNK, and p38. The phosphorylation levels dramatically increased at 2 hpi, followed by a decline to near basal levels by 24 hpi, as shown in a Bio-Plex assay and immunoblot analysis.
Formal Description
Interaction-ID: 36870

organism

Coxiella burnetii, phase II

increases_phosphorylation of

gene/protein

HSPB1

in THP-1 cells; at 2 h postinfection (hpi)
Comment The infection of THP-1 cells with C. burnetii phase II caused transient phosphorylation of c-Jun, Hsp27, JNK, and p38. The phosphorylation levels dramatically increased at 2 hpi, followed by a decline to near basal levels by 24 hpi, as shown in a Bio-Plex assay and immunoblot analysis.
Formal Description
Interaction-ID: 36871

organism

Coxiella burnetii, phase II

increases_activity of

gene/protein

MAPK8

in THP-1 cells; at 2 h postinfection (hpi)
Drugbank entries Show/Hide entries for MAPK8
Comment The infection of THP-1 cells with C. burnetii phase II caused transient phosphorylation of c-Jun, Hsp27, JNK, and p38. The phosphorylation levels dramatically increased at 2 hpi, followed by a decline to near basal levels by 24 hpi, as shown in a Bio-Plex assay and immunoblot analysis.
Formal Description
Interaction-ID: 36872

organism

Coxiella burnetii, phase II

increases_phosphorylation of

gene/protein

MAPK8

in THP-1 cells; at 2 h postinfection (hpi)
Drugbank entries Show/Hide entries for MAPK8
Comment The infection of THP-1 cells with C. burnetii phase II caused transient phosphorylation of c-Jun, Hsp27, JNK, and p38. The phosphorylation levels dramatically increased at 2 hpi, followed by a decline to near basal levels by 24 hpi, as shown in a Bio-Plex assay and immunoblot analysis.
Formal Description
Interaction-ID: 36873

organism

Coxiella burnetii, phase II

increases_activity of

gene/protein

p38 MAPK

in THP-1 cells; at 2 h postinfection (hpi)
Comment The infection of THP-1 cells with C. burnetii phase II caused transient phosphorylation of c-Jun, Hsp27, JNK, and p38. The phosphorylation levels dramatically increased at 2 hpi, followed by a decline to near basal levels by 24 hpi, as shown in a Bio-Plex assay and immunoblot analysis.
Formal Description
Interaction-ID: 36874

organism

Coxiella burnetii, phase II

increases_phosphorylation of

gene/protein

p38 MAPK

in THP-1 cells; at 2 h postinfection (hpi)
Comment The experiments show that Akt and Erk1/2 activation are C. burnetii-driven processes. Akt and Erk1/2 phosphorylation levels were elevated in THP-1 cells and Vero cells, infected with avirulent C. burnetii phase II and virulent C. burnetii phase I. Akt was phosphorylated at both S473 and T308 at 6 to 12 hpi, and phosphorylation was sustained through 48 hpi, ERK1/2 early after infection (2 hpi), with phosphorylation levels sustained through 48 hpi.
Formal Description
Interaction-ID: 36875

organism

Coxiella burnetii, phase II

increases_activity of

in THP-1 and Vero cells; from 6 h postinfection (hpi) to 48 hpi
Comment The experiments show that Akt and Erk1/2 activation are C. burnetii-driven processes. Akt and Erk1/2 phosphorylation levels were elevated in THP-1 cells and Vero cells, infected with avirulent C. burnetii phase II and virulent C. burnetii phase I. Akt was phosphorylated at both S473 and T308 at 6 to 12 hpi, and phosphorylation was sustained through 48 hpi, ERK1/2 early after infection (2 hpi), with phosphorylation levels sustained through 48 hpi.
Formal Description
Interaction-ID: 36877

organism

Coxiella burnetii, phase I

increases_activity of

in THP-1 and Vero cells; from 6 h postinfection (hpi) to 48 hpi
Comment The experiments show that Akt and Erk1/2 activation are C. burnetii-driven processes. Akt and Erk1/2 phosphorylation levels were elevated in THP-1 cells and Vero cells, infected with avirulent C. burnetii phase II and virulent C. burnetii phase I. Akt was phosphorylated at both S473 and T308 at 6 to 12 hpi, and phosphorylation was sustained through 48 hpi, ERK1/2 early after infection (2 hpi), with phosphorylation levels sustained through 48 hpi.
Formal Description
Interaction-ID: 36880

organism

Coxiella burnetii, phase II

increases_activity of

in THP-1 and Vero cells; from 2 h postinfection (hpi) to 48 hpi
Comment The experiments show that Akt and Erk1/2 activation are C. burnetii-driven processes. Akt and Erk1/2 phosphorylation levels were elevated in THP-1 cells and Vero cells, infected with avirulent C. burnetii phase II and virulent C. burnetii phase I. Akt was phosphorylated at both S473 and T308 at 6 to 12 hpi, and phosphorylation was sustained through 48 hpi, ERK1/2 early after infection (2 hpi), with phosphorylation levels sustained through 48 hpi.
Formal Description
Interaction-ID: 36884

organism

Coxiella burnetii, phase I

increases_activity of

in THP-1 and Vero cells; from 2 h postinfection (hpi) to 48 hpi
Comment C. burnetii requires Akt and Erk1/2 activation to protect mammalian cells from staurosporine-induced death. Experiments done in THP-1 cells, infected with phase II C. burnetii for 48 h in the presence or absence of LY294002 (class I PI3K inhibitor) or U0126 (MEK1/2 inhibitor) and then treated with staurosporine for 4 or 8 h.
Formal Description
Interaction-ID: 36890

decreases_activity of

process

host cell apoptosis

in THP-1 cells; infected with C. burnetii phase II, cell death induced with stauroporine
Comment C. burnetii requires Akt and Erk1/2 activation to protect mammalian cells from staurosporine-induced death. Experiments done in THP-1 cells, infected with phase II C. burnetii for 48 h in the presence or absence of LY294002 (class I PI3K inhibitor) or U0126 (MEK1/2 inhibitor) and then treated with staurosporine for 4 or 8 h.
Formal Description
Interaction-ID: 36891

decreases_activity of

process

host cell apoptosis

in THP-1 cells; infected with C. burnetii phase II, cell death induced with stauroporine