General Information:

Id: 3,770 (click here to show other Interactions for entry)
Diseases: Q fever
pathogen-host system
Coxiella burnetii/mammalia
bone marrow-derived macrophages (BMDMs), infected with virulent/avirulent (phase I/phase II) C. burnetii or virulent/avirulent LPS (vLPS/avLPS)
article
Reference: Barry AO et al.(2012) Impaired Stimulation of p38alpha-MAPK/Vps41-HOPS by LPS from Pathogenic Coxiella burnetii Prevents Trafficking to Microbicidal Phagolysosomes Cell Host Microbe 12: 751-763 [PMID: 23245320]

Interaction Information:

Comment Within a treatment of 30 min the authors analysed the absence of phosphorylated p38alpha-MAPK in BMDMs challenged with vLPS or vCb (virulent LPS or C. burnetii), in contrast to avLPS-or avCb (avirulent LPS or C. burnetii)-challenged BMDMs, in which phosphorylated p38a-MAPK could be detected. Thus p38alpha-MAPK can be defined as a key host factor differentially engaged during infection with avirulent and virulent strains of C. burnetii.
Formal Description
Interaction-ID: 36795

organism

Coxiella burnetii, phase II

increases_activity of

gene/protein

p38 MAPK

in BMDMs; infected with avirulent LPS or avirulent C. burnetii
Comment Within a treatment of 30 min the authors analysed the absence of phosphorylated p38alpha-MAPK in BMDMs challenged with vLPS or vCb (virulent LPS or C. burnetii), in contrast to avLPS-or avCb (avirulent LPS or C. burnetii)-challenged BMDMs, in which phosphorylated p38a-MAPK could be detected. Thus p38alpha-MAPK can be defined as a key host factor differentially engaged during infection with avirulent and virulent strains of C. burnetii.
Formal Description
Interaction-ID: 36796

organism

Coxiella burnetii, phase I

NOT increases_activity of

gene/protein

p38 MAPK

in BMDMs; infected with virulent LPS or virulent C. burnetii
Comment Within a treatment of 30 min the authors analysed the absence of phosphorylated p38alpha-MAPK in BMDMs challenged with vLPS or vCb (virulent LPS or C. burnetii), in contrast to avLPS-or avCb (avirulent LPS or C. burnetii)-challenged BMDMs, in which phosphorylated p38a-MAPK could be detected. Thus p38alpha-MAPK can be defined as a key host factor differentially engaged during infection with avirulent and virulent strains of C. burnetii.
Formal Description
Interaction-ID: 36797

organism

Coxiella burnetii, phase II

increases_phosphorylation of

gene/protein

p38 MAPK

in BMDMs; infected with avirulent LPS or avirulent C. burnetii
Comment Within a treatment of 30 min the authors analysed the absence of phosphorylated p38alpha-MAPK in BMDMs challenged with vLPS or vCb (virulent LPS or C. burnetii), in contrast to avLPS-or avCb (avirulent LPS or C. burnetii)-challenged BMDMs, in which phosphorylated p38a-MAPK could be detected. Thus p38alpha-MAPK can be defined as a key host factor differentially engaged during infection with avirulent and virulent strains of C. burnetii.
Formal Description
Interaction-ID: 36798

organism

Coxiella burnetii, phase I

NOT increases_phosphorylation of

gene/protein

p38 MAPK

in BMDMs; infected with virulent LPS or virulent C. burnetii
Comment The p38alpha-MAPK upstream activator MKK3/6 has not been induced in BMDMs stimulated with C. burnetii vLPS (virulent), but in BMDMs stimulated with C. burnetii avLPS (avirulent)
Formal Description
Interaction-ID: 36799

gene/protein

MAP2K3

increases_activity of

gene/protein

p38 MAPK

Comment The p38alpha-MAPK upstream activator MKK3/6 has not been induced in BMDMs stimulated with C. burnetii vLPS (virulent), but in BMDMs stimulated with C. burnetii avLPS (avirulent)
Formal Description
Interaction-ID: 36800

gene/protein

MAP2K6

increases_activity of

gene/protein

p38 MAPK

Comment The p38alpha-MAPK pathway-dependent trafficking of LPSs to lysosomes requires TLR4. TLR4 is required for vLPS (virulent) to inhibit p38a-MAPK phosphorylation.
Formal Description
Interaction-ID: 36806

gene/protein

TLR4

affects_activity of

gene/protein

p38 MAPK

in BMDMs; infected with virulent LPS
Drugbank entries Show/Hide entries for TLR4
Comment The results establish a critical role for p38alpha-MAPK signaling in C. burnetii fate and trafficking to lysosomes. avLPS (avirulent), but not vLPS (virulent), induces p38alpha-MAPK signaling, which drives endolysosomal conversion.
Formal Description
Interaction-ID: 36807

gene/protein

p38 MAPK

increases_activity of

in BMDMs and MEFs; infected with avirulent LPS
Comment Inhibition of p38alpha-MAPK blocked the phosphorylation of Vps41 in BMDMs challenged with avLPS (avirulent.
Formal Description
Interaction-ID: 36826

gene/protein

p38 MAPK

increases_activity of

gene/protein

VPS41

in BMDMs; infected with avirulent LPS
Comment Inhibition of p38alpha-MAPK blocked the phosphorylation of Vps41 in BMDMs challenged with avLPS (avirulent.
Formal Description
Interaction-ID: 36827

gene/protein

p38 MAPK

increases_phosphorylation of

gene/protein

VPS41

in BMDMs; infected with avirulent LPS
Comment p38alpha-MAPK coimmunoprecipitated with Vps41, and vice versa, demonstrating their interaction in avLPS (avirulent)-treated BMDMs .
Formal Description
Interaction-ID: 36829

gene/protein

p38 MAPK

interacts (colocalizes) with

gene/protein

VPS41

in BMDMs; infected with avirulent LPS
Comment The results establish a critical role for p38alpha-MAPK signaling in C. burnetii fate and trafficking to lysosomes. avLPS (avirulent), but not vLPS (virulent), induces p38alpha-MAPK signaling, which drives endolysosomal conversion.
Formal Description
Interaction-ID: 38611

gene/protein

p38 MAPK

NOT increases_activity of

in BMDMs and MEFs; infected with virulent LPS