General Information:

Id: 3,726 (click here to show other Interactions for entry)
Diseases: Q fever
pathogen-host system
Coxiella burnetii/mammalia
HeLa and Vero cells, infected with C. burnetii strain NMII, or the isogenic icmL::Tn mutant
article
Reference: Carey KL et al.(2011) The Coxiella burnetii Dot/Icm system delivers a unique repertoire of type IV effectors into host cells and is required for intracellular replication PLoS Pathog. 7 [PMID: 21637816]

Interaction Information:

Comment The Dot/Icm secretion system is essential for C. burnetii intracellular replication. The authors analysed the formation of large CCVs (Coxiella-containing vaculoles) containing replicating C. burnetii NMII, and a complete absence of large vacuoles containing replicating bacteria for the isogenic icmL::Tn mutant.
Formal Description
Interaction-ID: 36234

process

T4BSS

increases_activity of

process

Coxiella burnetii replication

in HeLa and Vero cells
Comment The icmL::Tn mutant has no obvious defect in replicating in defined ACCM medium or invading and surviving in cultured host cells.
Formal Description
Interaction-ID: 36238

process

T4BSS

NOT affects_activity of

process

pathogen survival

in HeLa and Vero cells
Comment The icmL::Tn mutant has no obvious defect in replicating in defined ACCM medium or invading and surviving in cultured host cells.
Formal Description
Interaction-ID: 36239

process

T4BSS

NOT affects_activity of

process

axenic cell growth

Comment In a beta-lactamase translocation assay, analysing delivered BlaM fusion proteins to the host cell cytosol, it was shown that BlaM-CBU0077, BlaM-CBU0635 and BlaM-CBU1524 were translocated into HeLa cells. No translocation-positive cells have been detected after host cells infection with the isogenic icmL::Tn mutant producing these fusion proteins. This demonstrates that the Dot/Icm apparatus is essential for the delivery of the effectors into the host cell.
Formal Description
Interaction-ID: 36240

process

T4BSS

increases_transport of

gene/protein

CBU_0077

into HeLa cells; shown in a BlaM translocation assay
Comment In a beta-lactamase translocation assay, analysing delivered BlaM fusion proteins to the host cell cytosol, it was shown that BlaM-CBU0077, BlaM-CBU0635 and BlaM-CBU1524 were translocated into HeLa cells. No translocation-positive cells have been detected after host cells infection with the isogenic icmL::Tn mutant producing these fusion proteins. This demonstrates that the Dot/Icm apparatus is essential for the delivery of the effectors into the host cell.
Formal Description
Interaction-ID: 36247

process

T4BSS

increases_transport of

gene/protein

CBU_0635

into HeLa cells; shown in a BlaM translocation assay
Comment In a beta-lactamase translocation assay, analysing delivered BlaM fusion proteins to the host cell cytosol, it was shown that BlaM-CBU0077, BlaM-CBU0635 and BlaM-CBU1524 were translocated into HeLa cells. No translocation-positive cells have been detected after host cells infection with the isogenic icmL::Tn mutant producing these fusion proteins. This demonstrates that the Dot/Icm apparatus is essential for the delivery of the effectors into the host cell.
Formal Description
Interaction-ID: 36248

process

T4BSS

increases_transport of

gene/protein

caeA

into HeLa cells; shown in a BlaM translocation assay