General Information:

Id: 3,683
Diseases: Pseudomonas aeruginosa diseases
pathogen-host system
Pseudomonas aeruginosa/mammalia
human bronchial epithelial cells, infected with P. aeruginosa strain PAO1
article
Reference: Gellatly SL et al.(2012) The Pseudomonas aeruginosa PhoP-PhoQ two-component regulatory system is induced upon interaction with epithelial cells and controls cytotoxicity and inflammation Infect. Immun. 80: 3122-3131 [PMID: 22710876]

Interaction Information:

Comment The PhoPQ system is substantially induced during adherence of wild-type Pseudomonas aeruginosa to human bronchial epithelial cells compared to planktonic cells that have failed to adhere. The PhoPQ system is a two-component regulatory system, with the sensor kinase PhoQ and the response regulator PhoP.
Formal Description
Interaction-ID: 35323

increases_activity of

gene/protein

phoQ

during adherence to HBE cells
Drugbank entries Show/Hide entries for phoQ
Comment The PhoPQ system is a two-component regulatory system, with the sensor kinase PhoQ and the response regulator PhoP.
Formal Description
Interaction-ID: 35343

gene/protein

phoQ

increases_activity of

gene/protein

phoP

Drugbank entries Show/Hide entries for phoQ
Comment Similar to P. aeruginosa strain PAO1, the environmental strain 62 demonstrated substantial upregulation of oprH, phoP, arnB, and pmrB during interaction with human bronchial epithelial cells (HBE). This genes belong to the PhoQ regulon. Arnb is involved in lipid modification, PmrB is known as a two-component regulator that also regulates the expression of arnB in response to limiting Mg2+ levels.
Formal Description
Interaction-ID: 35345

gene/protein

phoQ

increases_expression of

gene/protein

phoP

during adherence to HBE cells
Drugbank entries Show/Hide entries for phoQ
Comment Similar to P. aeruginosa strain PAO1, the environmental strain 62 demonstrated substantial upregulation of oprH, phoP, arnB, and pmrB during interaction with human bronchial epithelial cells (HBE). This genes belong to the PhoQ regulon. Arnb is involved in lipid modification, PmrB is known as a two-component regulator that also regulates the expression of arnB in response to limiting Mg2+ levels.
Formal Description
Interaction-ID: 35347

gene/protein

phoQ

increases_expression of

gene/protein

oprH

during adherence to HBE cells
Drugbank entries Show/Hide entries for phoQ
Comment Similar to P. aeruginosa strain PAO1, the environmental strain 62 demonstrated substantial upregulation of oprH, phoP, arnB, and pmrB during interaction with human bronchial epithelial cells (HBE). This genes belong to the PhoQ regulon. Arnb is involved in lipid modification, PmrB is known as a two-component regulator that also regulates the expression of arnB in response to limiting Mg2+ levels.
Formal Description
Interaction-ID: 35348

gene/protein

phoQ

increases_expression of

gene/protein

pmrB

during adherence to HBE cells
Drugbank entries Show/Hide entries for phoQ
Comment Similar to P. aeruginosa strain PAO1, the environmental strain 62 demonstrated substantial upregulation of oprH, phoP, arnB, and pmrB during interaction with human bronchial epithelial cells (HBE). This genes belong to the PhoQ regulon. Arnb is involved in lipid modification, PmrB is known as a two-component regulator that also regulates the expression of arnB in response to limiting Mg2+ levels.
Formal Description
Interaction-ID: 35351

gene/protein

phoQ

increases_expression of

gene/protein

arnB

during adherence to HBE cells
Drugbank entries Show/Hide entries for phoQ or arnB
Comment Several virulence factors were downregulated in a phoQ mutant during interaction with human epithelial cells in vitro, based on qPCR analysis. Elastase B (lasB) showed the biggest difference from the WT, (26-fold-reduction).
Formal Description
Interaction-ID: 35362

gene/protein

phoQ

increases_expression of

gene/protein

lasB

during adherence to HBE cells
Drugbank entries Show/Hide entries for phoQ or lasB
Comment Several virulence factors were downregulated in a phoQ mutant during interaction with human epithelial cells in vitro, based on qPCR analysis. Protease IV (piv) showed a 3-fold downregulation.
Formal Description
Interaction-ID: 35365

gene/protein

phoQ

increases_expression of

gene/protein

piv

during adherence to HBE cells
Drugbank entries Show/Hide entries for phoQ
Comment Several virulence factors were downregulated in a phoQ mutant during interaction with human epithelial cells in vitro, based on qPCR analysis. Lipases lipA and lipC, as well as the lipase modulator lipH, which controls both LipA and LipC, were downregulated in the phoQ mutant by 3- to 5.7-fold.
Formal Description
Interaction-ID: 35366

gene/protein

phoQ

increases_expression of

gene/protein

lipA

during adherence to HBE cells
Drugbank entries Show/Hide entries for phoQ or lipA
Comment Several virulence factors were downregulated in a phoQ mutant during interaction with human epithelial cells in vitro, based on qPCR analysis. Lipases lipA and lipC, as well as the lipase modulator lipH, which controls both LipA and LipC, were downregulated in the phoQ mutant by 3- to 5.7-fold.
Formal Description
Interaction-ID: 35368

gene/protein

phoQ

increases_expression of

gene/protein

lipC

during adherence to HBE cells
Drugbank entries Show/Hide entries for phoQ
Comment Several virulence factors were downregulated in a phoQ mutant during interaction with human epithelial cells in vitro, based on qPCR analysis. Lipases lipA and lipC, as well as the lipase modulator lipH, which controls both LipA and LipC, were downregulated in the phoQ mutant by 3- to 5.7-fold.
Formal Description
Interaction-ID: 35371

gene/protein

phoQ

increases_expression of

gene/protein

lipH

during adherence to HBE cells
Drugbank entries Show/Hide entries for phoQ
Comment The lipases LipA, LipC and phospholipases have been shown to break down lung surfactant, which is composed of 90% lipid.
Formal Description
Interaction-ID: 35376

gene/protein

lipA

decreases_activity of

Drugbank entries Show/Hide entries for lipA
Comment The lipases LipA, LipC and phospholipases have been shown to break down lung surfactant, which is composed of 90% lipid.
Formal Description
Interaction-ID: 35377

gene/protein

lipC

decreases_activity of

Comment There was no dysregulation in a phoQ mutant concerning the expression of exoS (T3SS) or toxA (T2SS) during interaction with human epithelial cells in vitro.
Formal Description
Interaction-ID: 35379

gene/protein

phoQ

NOT affects_expression of

gene/protein

exoS

Drugbank entries Show/Hide entries for phoQ
Comment There was no dysregulation in a phoQ mutant concerning the expression of exoS (T3SS) or toxA (T2SS) during interaction with human epithelial cells in vitro.
Formal Description
Interaction-ID: 35381

gene/protein

phoQ

NOT affects_expression of

gene/protein

toxA

Drugbank entries Show/Hide entries for phoQ or toxA
Comment Similar to P. aeruginosa strain PAO1, the environmental strain 62 demonstrated substantial upregulation of oprH, phoP, arnB, and pmrB during interaction with human bronchial epithelial cells (HBE). This genes belong to the PhoQ regulon. Arnb is involved in lipid modification, PmrB is known as a two-component regulator that also regulates the expression of arnB in response to limiting Mg2+ levels.
Formal Description
Interaction-ID: 35382

gene/protein

arnB

affects_activity of

Drugbank entries Show/Hide entries for arnB
Comment PhoQ mutation led to altered structure and inflammatory properties of the lipid A portion of lipopolysaccharid (LPS). This indicates that the mutation of phoQ results in a phenotype that is similar to the less virulent but more inflammatory phenotype of clinical strains isolated from chronic-stage cystic fibrosis lung infections.
Formal Description
Interaction-ID: 35385

gene/protein

phoQ

affects_activity of

Drugbank entries Show/Hide entries for phoQ
Comment The PhoPQ system is substantially induced during adherence of wild-type Pseudomonas aeruginosa to human bronchial epithelial cells compared to planktonic cells that have failed to adhere. The PhoPQ system is a two-component regulatory system, with the sensor kinase PhoQ and the response regulator PhoP.
Formal Description
Interaction-ID: 35388

process

P. aeruginosa infection

increases_activity of