General Information:

Id: 3,577
Diseases: Pseudomonas aeruginosa diseases
pathogen-host system
Pseudomonas aeruginosa/mammalia
P. aeruginosa strain PAO1
article
Reference: Luckett JC et al.(2012) A Novel Virulence Strategy for Pseudomonas aeruginosa Mediated by an Autotransporter with Arginine-Specific Aminopeptidase Activity PLoS Pathog. 8 [PMID: 22927813]

Interaction Information:

Comment The autotransporter PA0328, renamed to AaaA, has been characterized as a cell-surface tethered, arginine-specific aminopeptidase.
Formal Description
Interaction-ID: 33495

gene/protein

aaaA

is localized in

cellular component

bacterial outer membrane

Comment Autotransporters appear to have the simplest mechanism of secretion found in Gram-negative bacteria and constitute a subgroup of the type V secretion system. PA0328 (AaaA) has been characterized as autotransporter.
Formal Description
Interaction-ID: 33496

process

T5aSS

increases_transport of

gene/protein

aaaA

Comment The delta-aaaA mutant showed significantly reduced survival in a mouse wound chronic infection in comparison to wild type both 2 days and 8 days post infection.
Formal Description
Interaction-ID: 33497

gene/protein

aaaA

increases_activity of

process

P. aeruginosa infection, chronic

2 days and 8 days post infection
Comment It was shown that the autotransporter PA0328 (AaaA) provides a fitness advantage in environments where the sole source of nitrogen is peptides with an aminoterminal arginine. The delta-aaaA mutant is unable to grow when the sole source of carbon and nitrogen is provided by peptides with amino terminal arginine.
Formal Description
Interaction-ID: 33498

gene/protein

aaaA

affects_activity of

by using peptides with an aminoterminal arginine as sole nitrogen source
Comment Experiments revealed lower levels of expression of the pro-inflammatory cytokines TNF-alpha and IL-1-alpha in mice colonised with the delta-aaaA mutant in comparison to the wild type, as shown by qRT-PCR.
Formal Description
Interaction-ID: 33503

gene/protein

aaaA

increases_quantity of

gene/protein

TNF

shown by qRT-PCR analysis, 2 days post infection
Drugbank entries Show/Hide entries for TNF
Comment Experiments revealed lower levels of expression of the pro-inflammatory cytokines TNF-alpha and IL-1-alpha in mice colonised with the delta-aaaA mutant in comparison to the wild type, as shown by qRT-PCR.
Formal Description
Interaction-ID: 33504

gene/protein

aaaA

increases_quantity of

gene/protein

IL1A

shown by qRT-PCR analysis, 2 days post infection
Comment Experiments revealed lower levels of expression of the keratinocyte-derived cytokine (KC) and COX-2 in mice colonised with the delta-aaaA mutant in comparison to the wild type, as shown by qRT-PCR.
Formal Description
Interaction-ID: 33505

gene/protein

aaaA

increases_quantity of

gene/protein

Keratinocyte-derived cytokine

shown by qRT-PCR analysis, 2 days post infection
Comment Experiments revealed lower levels of expression of the keratinocyte-derived cytokine (KC) and COX-2 in mice colonised with the delta-aaaA mutant in comparison to the wild type, as shown by qRT-PCR.
Formal Description
Interaction-ID: 33506

gene/protein

aaaA

increases_quantity of

gene/protein

PTGS2

shown by qRT-PCR analysis, 2 days post infection
Drugbank entries Show/Hide entries for PTGS2
Comment It has been shown that the arginine-specific aminopeptidase AaaA released arginine from the aminoterminus of di and tripeptides. P. aeruginosa was able to use the liberated arginine as a nutrient for growth, providing a fitness advantage when arginine-containing peptides were the sole source of nitrogen in the environment.
Formal Description
Interaction-ID: 33518

gene/protein

aaaA

increases_quantity of

drug/chemical compound

Arginine

Comment It has been shown that the arginine-specific aminopeptidase AaaA released arginine from the aminoterminus of di and tripeptides. P. aeruginosa was able to use the liberated arginine as a nutrient for growth, providing a fitness advantage when arginine-containing peptides were the sole source of nitrogen in the environment.
Formal Description
Interaction-ID: 33519

drug/chemical compound

Arginine

affects_activity of

Comment The arginine-specific aminopeptidase AaaA did not confer a virulence advantage in an acute burn wound infection as it did in a chronic wound infection, shown in colonisation experiments.
Formal Description
Interaction-ID: 33520

gene/protein

aaaA

NOT affects_activity of

process

P. aeruginosa infection, acute