General Information:

Id: 3,572
Diseases: Q fever
pathogen-host system
Coxiella burnetii/mammalia
HMDMs and THP-1 cells, infected with C. burnetii phase I variant (NMI) or phase II variant (NMII)
article
Reference: Howe D et al.(2010) Coxiella burnetii phase I and II variants replicate with similar kinetics in degradative phagolysosome-like compartments of human macrophages Infect. Immun. 78: 3465-3474 [PMID: 20515926]

Interaction Information:

Comment C. burnetii variants NMI and NMII replicate with similar kinetics in HMDM (primary human monocyte-derived macrophages) and THP-1 (human monocyte-like) cells.
Formal Description
Interaction-ID: 33396

organism

Coxiella burnetii, phase I

increases_activity of

process

Coxiella burnetii replication

in THP-1 and HMDM cells
Comment C. burnetii variants NMI and NMII replicate with similar kinetics in HMDM (primary human monocyte-derived macrophages) and THP-1 (human monocyte-like) cells.
Formal Description
Interaction-ID: 33397

organism

Coxiella burnetii, phase II

increases_activity of

process

Coxiella burnetii replication

in THP-1 and HMDM cells
Comment HMDMs (primary human monocyte-derived macrophages) infected with C. burnetii variants NMI and NMII secrete comparable levels of proinflammatory cytokines TNF-alpha and IL-6.
Formal Description
Interaction-ID: 33398

organism

Coxiella burnetii, phase I

increases_quantity of

gene/protein

TNF

in HMDM cells
Drugbank entries Show/Hide entries for TNF
Comment HMDMs (primary human monocyte-derived macrophages) infected with C. burnetii variants NMI and NMII secrete comparable levels of proinflammatory cytokines TNF-alpha and IL-6.
Formal Description
Interaction-ID: 33399

organism

Coxiella burnetii, phase II

increases_quantity of

gene/protein

TNF

in HMDM cells
Drugbank entries Show/Hide entries for TNF
Comment HMDMs (primary human monocyte-derived macrophages) infected with C. burnetii variants NMI and NMII secrete comparable levels of proinflammatory cytokines TNF-alpha and IL-6.
Formal Description
Interaction-ID: 33400

organism

Coxiella burnetii, phase I

increases_quantity of

gene/protein

IL6

in HMDM cells
Drugbank entries Show/Hide entries for IL6
Comment HMDMs (primary human monocyte-derived macrophages) infected with C. burnetii variants NMI and NMII secrete comparable levels of proinflammatory cytokines TNF-alpha and IL-6.
Formal Description
Interaction-ID: 33401

organism

Coxiella burnetii, phase II

increases_quantity of

gene/protein

IL6

in HMDM cells
Drugbank entries Show/Hide entries for IL6
Comment C. burnetii variants NMI and NMII replicate within the same PVs (parasitophorous vacuole) in HMDM (primary human monocyte-derived macrophages) and THP-1 (human monocyte-like) cells.
Formal Description
Interaction-ID: 33402

organism

Coxiella burnetii, phase I

is localized in

cellular component

C. burnetii containing vacuole, CCV

in THP-1 and HMDM cells
Comment C. burnetii variants NMI and NMII replicate within the same PVs (parasitophorous vacuole) in HMDM (primary human monocyte-derived macrophages) and THP-1 (human monocyte-like) cells.
Formal Description
Interaction-ID: 33427

organism

Coxiella burnetii, phase II

is localized in

cellular component

C. burnetii containing vacuole, CCV

in THP-1 and HMDM cells
Comment PVs (parasitophorous vacuole) harboring C. burnetii variants NMI or NMII decorate similarly with endolysosomal markers, Rab5, as early endosome marker, Rab7,as late endosome marker, CD63, as late endosome/lysosome marker and the lysosome marker cathepsin D. The results suggest that PVs harboring NMI or NMII mature similarly through the endolysosomal pathway to ultimately acquire characteristics of a phagolysosome.
Formal Description
Interaction-ID: 33428

organism

Coxiella burnetii, phase I

increases_activity of

in THP-1 cells
Comment PVs (parasitophorous vacuole) harboring C. burnetii variants NMI or NMII decorate similarly with endolysosomal markers, Rab5, as early endosome marker, Rab7,as late endosome marker, CD63, as late endosome/lysosome marker and the lysosome marker cathepsin D. The results suggest that PVs harboring NMI or NMII mature similarly through the endolysosomal pathway to ultimately acquire characteristics of a phagolysosome.
Formal Description
Interaction-ID: 33429

organism

Coxiella burnetii, phase II

increases_activity of

in THP-1 cells
Comment PVs (parasitophorous vacuole) harboring C. burnetii variants NMI or NMII decorate similarly with endolysosomal markers, Rab5, as early endosome marker, Rab7,as late endosome marker, CD63, as late endosome/lysosome marker and the lysosome marker cathepsin D. The results suggest that PVs harboring NMI or NMII mature similarly through the endolysosomal pathway to ultimately acquire characteristics of a phagolysosome.
Formal Description
Interaction-ID: 33430

organism

Coxiella burnetii, phase I

increases_activity of

in THP-1 cells
Comment PVs (parasitophorous vacuole) harboring C. burnetii variants NMI or NMII decorate similarly with endolysosomal markers, Rab5, as early endosome marker, Rab7,as late endosome marker, CD63, as late endosome/lysosome marker and the lysosome marker cathepsin D. The results suggest that PVs harboring NMI or NMII mature similarly through the endolysosomal pathway to ultimately acquire characteristics of a phagolysosome.
Formal Description
Interaction-ID: 33431

organism

Coxiella burnetii, phase II

increases_activity of

in THP-1 cells
Comment This study demonstrates that PVs (parasitophorous vacuole) harboring C. burnetii variants NMI or NMII are proteolytically active and that both cysteine and aspartate cathepsins contribute to proteolysis.
Formal Description
Interaction-ID: 33432

cellular component

C. burnetii containing vacuole, CCV

increases_activity of

process

proteolysis

in THP-1 cells in PVs of both C. burnetii variant NMI or NMII
Comment Proteolytically active cathepsin D contributes to PV (parasitophorous vacuole) degradative activity.
Formal Description
Interaction-ID: 33433

gene/protein

CTSD

increases_activity of

process

proteolysis

in THP-1 cells in PVs of both C. burnetii variant NMI or NMII
Drugbank entries Show/Hide entries for CTSD
Comment Proteolytically active cathepsin B, K and L are present in the PV (parasitophorous vacuole) of THP-1 cells infected with C. burnetii strain variants NMI or NMII.
Formal Description
Interaction-ID: 33434

gene/protein

CTSB

increases_activity of

process

proteolysis

in THP-1 cells in PVs of both C. burnetii variant NMI or NMII
Drugbank entries Show/Hide entries for CTSB
Comment Proteolytically active cathepsin B, K and L are present in the PV (parasitophorous vacuole) of THP-1 cells infected with C. burnetii strain variants NMI or NMII.
Formal Description
Interaction-ID: 33435

gene/protein

CTSB

is localized in

cellular component

C. burnetii containing vacuole, CCV

in THP-1 cells in PVs of both C. burnetii variant NMI or NMII
Drugbank entries Show/Hide entries for CTSB
Comment Proteolytically active cathepsin B, K and L are present in the PV (parasitophorous vacuole) of THP-1 cells infected with C. burnetii strain variants NMI or NMII.
Formal Description
Interaction-ID: 33436

gene/protein

CTSK

increases_activity of

process

proteolysis

in THP-1 cells in PVs of both C. burnetii variant NMI or NMII
Drugbank entries Show/Hide entries for CTSK
Comment Proteolytically active cathepsin B, K and L are present in the PV (parasitophorous vacuole) of THP-1 cells infected with C. burnetii strain variants NMI or NMII.
Formal Description
Interaction-ID: 33437

gene/protein

CTSK

is localized in

cellular component

C. burnetii containing vacuole, CCV

in THP-1 cells in PVs of both C. burnetii variant NMI or NMII
Drugbank entries Show/Hide entries for CTSK
Comment Proteolytically active cathepsin B, K and L are present in the PV (parasitophorous vacuole) of THP-1 cells infected with C. burnetii strain variants NMI or NMII.
Formal Description
Interaction-ID: 33438

gene/protein

CTSL

increases_activity of

process

proteolysis

in THP-1 cells in PVs of both C. burnetii variant NMI or NMII
Comment Proteolytically active cathepsin B, K and L are present in the PV (parasitophorous vacuole) of THP-1 cells infected with C. burnetii strain variants NMI or NMII.
Formal Description
Interaction-ID: 33439

gene/protein

CTSL

is localized in

cellular component

C. burnetii containing vacuole, CCV

in THP-1 cells in PVs of both C. burnetii variant NMI or NMII
Comment PVs (parasitophorous vacuole) harboring C. burnetii variants NMI or NMII decorate similarly with endolysosomal markers, Rab5, as early endosome marker, Rab7,as late endosome marker, CD63, as late endosome/lysosome marker and the lysosome marker cathepsin D. The results suggest that PVs harboring NMI or NMII mature similarly through the endolysosomal pathway to ultimately acquire characteristics of a phagolysosome.
Formal Description
Interaction-ID: 37004

organism

Coxiella burnetii, phase II

interacts (colocalizes) with

gene/protein

RAB5

in THP-1 cells
Comment PVs (parasitophorous vacuole) harboring C. burnetii variants NMI or NMII decorate similarly with endolysosomal markers, Rab5, as early endosome marker, Rab7,as late endosome marker, CD63, as late endosome/lysosome marker and the lysosome marker cathepsin D. The results suggest that PVs harboring NMI or NMII mature similarly through the endolysosomal pathway to ultimately acquire characteristics of a phagolysosome.
Formal Description
Interaction-ID: 37006

organism

Coxiella burnetii, phase II

interacts (colocalizes) with

gene/protein

RAB7

in THP-1 cells
Comment PVs (parasitophorous vacuole) harboring C. burnetii variants NMI or NMII decorate similarly with endolysosomal markers, Rab5, as early endosome marker, Rab7,as late endosome marker, CD63, as late endosome/lysosome marker and the lysosome marker cathepsin D. The results suggest that PVs harboring NMI or NMII mature similarly through the endolysosomal pathway to ultimately acquire characteristics of a phagolysosome.
Formal Description
Interaction-ID: 37007

organism

Coxiella burnetii, phase II

interacts (colocalizes) with

gene/protein

CD63

in THP-1 cells
Comment PVs (parasitophorous vacuole) harboring C. burnetii variants NMI or NMII decorate similarly with endolysosomal markers, Rab5, as early endosome marker, Rab7,as late endosome marker, CD63, as late endosome/lysosome marker and the lysosome marker cathepsin D. The results suggest that PVs harboring NMI or NMII mature similarly through the endolysosomal pathway to ultimately acquire characteristics of a phagolysosome.
Formal Description
Interaction-ID: 37008

organism

Coxiella burnetii, phase II

interacts (colocalizes) with

gene/protein

CTSD

in THP-1 cells
Drugbank entries Show/Hide entries for CTSD
Comment PVs (parasitophorous vacuole) harboring C. burnetii variants NMI or NMII decorate similarly with endolysosomal markers, Rab5, as early endosome marker, Rab7,as late endosome marker, CD63, as late endosome/lysosome marker and the lysosome marker cathepsin D. The results suggest that PVs harboring NMI or NMII mature similarly through the endolysosomal pathway to ultimately acquire characteristics of a phagolysosome.
Formal Description
Interaction-ID: 37009

organism

Coxiella burnetii, phase I

interacts (colocalizes) with

gene/protein

RAB5

in THP-1 cells
Comment PVs (parasitophorous vacuole) harboring C. burnetii variants NMI or NMII decorate similarly with endolysosomal markers, Rab5, as early endosome marker, Rab7,as late endosome marker, CD63, as late endosome/lysosome marker and the lysosome marker cathepsin D. The results suggest that PVs harboring NMI or NMII mature similarly through the endolysosomal pathway to ultimately acquire characteristics of a phagolysosome.
Formal Description
Interaction-ID: 37010

organism

Coxiella burnetii, phase I

interacts (colocalizes) with

gene/protein

RAB7

in THP-1 cells
Comment PVs (parasitophorous vacuole) harboring C. burnetii variants NMI or NMII decorate similarly with endolysosomal markers, Rab5, as early endosome marker, Rab7,as late endosome marker, CD63, as late endosome/lysosome marker and the lysosome marker cathepsin D. The results suggest that PVs harboring NMI or NMII mature similarly through the endolysosomal pathway to ultimately acquire characteristics of a phagolysosome.
Formal Description
Interaction-ID: 37011

organism

Coxiella burnetii, phase I

interacts (colocalizes) with

gene/protein

CD63

in THP-1 cells
Comment PVs (parasitophorous vacuole) harboring C. burnetii variants NMI or NMII decorate similarly with endolysosomal markers, Rab5, as early endosome marker, Rab7,as late endosome marker, CD63, as late endosome/lysosome marker and the lysosome marker cathepsin D. The results suggest that PVs harboring NMI or NMII mature similarly through the endolysosomal pathway to ultimately acquire characteristics of a phagolysosome.
Formal Description
Interaction-ID: 37012

organism

Coxiella burnetii, phase I

interacts (colocalizes) with

gene/protein

CTSD

in THP-1 cells
Drugbank entries Show/Hide entries for CTSD