General Information:

Id: 3,568
Diseases: Q fever
pathogen-host system
Coxiella burnetii/mammalia
THP-1 cells, or HeLa cells, infected with avirulent C. burnetii phase II
article
Reference: Hussain SK et al.(2010) Host Kinase Activity is Required for Coxiella burnetii Parasitophorous Vacuole Formation Front Microbiol 1: 137 [PMID: 21772829]

Interaction Information:

Comment The results show that numerous signaling proteins, including PRL1, PKC, PKA, and MLCK are involved in PV maturation events and do not have bactericidal effects on C. burnetii. The removing of the corresponding inhibitors from the infected cells resulted in PV maturation, with CD63-positive vacuoles present at 48 h post-washout.
Formal Description
Interaction-ID: 33348

gene/protein

PTP4A1

increases_activity of

Comment The results show that numerous signaling proteins, including PRL1, PKC, PKA, and MLCK are involved in PV maturation events and do not have bactericidal effects on C. burnetii. The removing of the corresponding inhibitors from the infected cells resulted in PV maturation, with CD63-positive vacuoles present at 48 h post-washou.
Formal Description
Interaction-ID: 33349

gene/protein

Protein kinase C

increases_activity of

Comment The results show that numerous signaling proteins, including PRL1, PKC, PKA, and MLCK are involved in PV maturation events and do not have bactericidal effects on C. burnetii. The removing of the corresponding inhibitors from the infected cells resulted in PV maturation, with CD63-positive vacuoles present at 48 h post-washou.
Formal Description
Interaction-ID: 33350

complex/PPI

Protein kinase A

increases_activity of

Comment The results show that numerous signaling proteins, including PRL1, PKC, PKA, and MLCK are involved in PV maturation events and do not have bactericidal effects on C. burnetii. The removing of the corresponding inhibitors from the infected cells resulted in PV maturation, with CD63-positive vacuoles present at 48 h post-washout.
Formal Description
Interaction-ID: 33351

gene/protein

MYLK

increases_activity of

Comment C. burnetii infection, using avirulent phase II organisms, causes differential phosphorylation of p38 and myosin light chain-2 (MLC-2), the downstream target of MLCK. The levels of phosphorylated p38 increased over those of uninfected cells at 2 hpi, then declined to near basal level between 48 and 72 hpi.
Formal Description
Interaction-ID: 33352

organism

Coxiella burnetii, phase II

increases_activity of

gene/protein

p38 MAPK

in THP-1 cells
Comment C. burnetii infection, using avirulent phase II organisms, causes differential phosphorylation of p38 and myosin light chain-2 (MLC-2), the downstream target of MLCK. The levels of phosphorylated p38 increased over those of uninfected cells at 2 hpi, then declined to near basal level between 48 and 72 hpi.
Formal Description
Interaction-ID: 33353

organism

Coxiella burnetii, phase II

increases_phosphorylation of

gene/protein

p38 MAPK

in THP-1 cells
Comment C. burnetii infection, using avirulent phase II organisms, causes differential phosphorylation of p38 and myosin light chain-2 (MLC-2), the downstream target of MLCK. MLC-2 was also phosphorylated by 3 hpi, however, levels continued to increase throughout infection.
Formal Description
Interaction-ID: 33354

organism

Coxiella burnetii, phase II

increases_activity of

gene/protein

MYLK

in THP-1 cells
Comment C. burnetii infection causes differential phosphorylation of p38 and myosin light chain-2 (MLC-2), the downstream target of MLCK. MLC-2 was also phosphorylated by 3 hpi, however, levels continued to increase throughout infection.
Formal Description
Interaction-ID: 33355

gene/protein

MYLK

increases_phosphorylation of

gene/protein

MYL2

Comment Protein kinase A is differentially activated during infection with phase II (avirulent) C. burnetii. The phosphorylation levels of three PKA substrates substantially decreased by 48-72 hpi, in contrast to four proteins, which exhibited increased phosphorylation at later times of infection (24-96 hpi).
Formal Description
Interaction-ID: 33356

organism

Coxiella burnetii, phase II

increases_activity of

complex/PPI

Protein kinase A

in THP-1 cells
Comment C. burnetii infection with phase I (virulent) or phase II (avirulent) isolates trigger prolonged PKC activation. Infection with phase II (avirulent) isolates show increased PKC substrate phosphorylation through 96 hpi and MARCKs phosphorylation through 120 hpi.
Formal Description
Interaction-ID: 33358

organism

Coxiella burnetii, phase II

increases_activity of

gene/protein

Protein kinase C

in THP-1 cells
Comment The results show that numerous signaling proteins, including PRL1, PKC, PKA, and MLCK are involved in PV maturation events and do not have bactericidal effects on C. burnetii. The removing of the corresponding inhibitors from the infected cells resulted in PV maturation, with CD63-positive vacuoles present at 48 h post-washout.
Formal Description
Interaction-ID: 33381

organism

Coxiella burnetii, phase II

increases_activity of

gene/protein

PTP4A1

in THP-1 cells
Comment The PKC-specific substrate MARCKS was phosphorylated at 24 h post-infection and remained phosphorylated through 5 days post-infection, indicating prolonged regulation of the PKC pathway by C. burnetii. C. burnetii infection experiments performed with virulent (phase I) and avirulent (phase II) strains.
Formal Description
Interaction-ID: 33382

gene/protein

Protein kinase C

increases_activity of

gene/protein

MARCKS

in THP-1 cells; experiments done with virulent and avirulent strains
Comment The PKC-specific substrate MARCKS was phosphorylated at 24 h post-infection and remained phosphorylated through 5 days post-infection, indicating prolonged regulation of the PKC pathway by C. burnetii. C. burnetii infection experiments performed with virulent (phase I) and avirulent (phase II) strains.
Formal Description
Interaction-ID: 33383

gene/protein

Protein kinase C

increases_phosphorylation of

gene/protein

MARCKS

in THP-1 cells; experiments done with virulent and avirulent strains
Comment C. burnetii infection causes differential phosphorylation of p38 and myosin light chain-2 (MLC-2), the downstream target of MLCK. MLC-2 was also phosphorylated by 3 hpi, however, levels continued to increase throughout infection.
Formal Description
Interaction-ID: 33385

gene/protein

MYLK

increases_activity of

gene/protein

MYL2

Comment C. burnetii infection with phase I (virulent) or phase II (avirulent) isolates trigger prolonged PKC activation. Infection with phase I (virulent) isolates show increased levels of phosphorylated MARCKs, indicating these organisms activate PKC during intracellular growt.
Formal Description
Interaction-ID: 36865

organism

Coxiella burnetii, phase I

increases_activity of

gene/protein

Protein kinase C

in THP-1 cells