General Information:

Id: 3,521
Diseases: Q fever
pathogen-host system
Coxiella burnetii/mammalia
review
Reference: McDonough JA et al.(2012) Coxiella burnetii Secretion Systems Adv. Exp. Med. Biol. 984: 171-197 [PMID: 22711632]

Interaction Information:

Comment The Dot/Icm type IV secretion system has been analysed in C. burnetii in homology to L. pneumophila. Examination of the relative transcript levels concerning the three operons icmW-CBU1651-icmX, icmV-dotA-CBU1647 and icmT-icmSicmD-icmC-icmB-CBU1646 showed an increased transcription of these genes during the initial 16 h of C. burnetii infection of a eukaryotic host cell, and a subsequent drop in transcript levels by 24 h post-infection.
Formal Description
Interaction-ID: 32721

process

Coxiella burnetii infection

increases_expression of

gene/protein

icmW

during the initial 16 h of infection
Comment The Dot/Icm type IV secretion system has been analysed in C. burnetii in homology to L. pneumophila. Examination of the relative transcript levels concerning the three operons icmW-CBU1651-icmX, icmV-dotA-CBU1647 and icmT-icmSicmD-icmC-icmB-CBU1646 showed an increased transcription of these genes during the initial 16 h of C. burnetii infection of a eukaryotic host cell, and a subsequent drop in transcript levels by 24 h post-infection.
Formal Description
Interaction-ID: 32739

process

Coxiella burnetii infection

increases_expression of

gene/protein

CBU_1651

during the initial 16 h of infection
Comment The Dot/Icm type IV secretion system has been analysed in C. burnetii in homology to L. pneumophila. Examination of the relative transcript levels concerning the three operons icmW-CBU1651-icmX, icmV-dotA-CBU1647 and icmT-icmSicmD-icmC-icmB-CBU1646 showed an increased transcription of these genes during the initial 16 h of C. burnetii infection of a eukaryotic host cell, and a subsequent drop in transcript levels by 24 h post-infection.
Formal Description
Interaction-ID: 32741

process

Coxiella burnetii infection

increases_expression of

gene/protein

icmX

during the initial 16 h of infection
Comment The Dot/Icm type IV secretion system has been analysed in C. burnetii in homology to L. pneumophila. Examination of the relative transcript levels concerning the three operons icmW-CBU1651-icmX, icmV-dotA-CBU1647 and icmT-icmSicmD-icmC-icmB-CBU1646 showed an increased transcription of these genes during the initial 16 h of C. burnetii infection of a eukaryotic host cell, and a subsequent drop in transcript levels by 24 h post-infection.
Formal Description
Interaction-ID: 32743

process

Coxiella burnetii infection

increases_expression of

gene/protein

icmV

during the initial 16 h of infection
Comment The Dot/Icm type IV secretion system has been analysed in C. burnetii in homology to L. pneumophila. Examination of the relative transcript levels concerning the three operons icmW-CBU1651-icmX, icmV-dotA-CBU1647 and icmT-icmSicmD-icmC-icmB-CBU1646 showed an increased transcription of these genes during the initial 16 h of C. burnetii infection of a eukaryotic host cell, and a subsequent drop in transcript levels by 24 h post-infection.
Formal Description
Interaction-ID: 32744

process

Coxiella burnetii infection

increases_expression of

gene/protein

dotaA

during the initial 16 h of infection
Comment The Dot/Icm type IV secretion system has been analysed in C. burnetii in homology to L. pneumophila. Examination of the relative transcript levels concerning the three operons icmW-CBU1651-icmX, icmV-dotA-CBU1647 and icmT-icmSicmD-icmC-icmB-CBU1646 showed an increased transcription of these genes during the initial 16 h of C. burnetii infection of a eukaryotic host cell, and a subsequent drop in transcript levels by 24 h post-infection.
Formal Description
Interaction-ID: 32745

process

Coxiella burnetii infection

increases_expression of

gene/protein

CBU_1647

during the initial 16 h of infection
Comment The Dot/Icm type IV secretion system has been analysed in C. burnetii in homology to L. pneumophila. Examination of the relative transcript levels concerning the three operons icmW-CBU1651-icmX, icmV-dotA-CBU1647 and icmT-icmSicmD-icmC-icmB-CBU1646 showed an increased transcription of these genes during the initial 16 h of C. burnetii infection of a eukaryotic host cell, and a subsequent drop in transcript levels by 24 h post-infection.
Formal Description
Interaction-ID: 32748

process

Coxiella burnetii infection

increases_expression of

gene/protein

icmT

during the initial 16 h of infection
Comment The Dot/Icm type IV secretion system has been analysed in C. burnetii in homology to L. pneumophila. Examination of the relative transcript levels concerning the three operons icmW-CBU1651-icmX, icmV-dotA-CBU1647 and icmT-icmSicmD-icmC-icmB-CBU1646 showed an increased transcription of these genes during the initial 16 h of C. burnetii infection of a eukaryotic host cell, and a subsequent drop in transcript levels by 24 h post-infection.
Formal Description
Interaction-ID: 32749

process

Coxiella burnetii infection

increases_expression of

gene/protein

icmS

during the initial 16 h of infection
Comment The Dot/Icm type IV secretion system has been analysed in C. burnetii in homology to L. pneumophila. Examination of the relative transcript levels concerning the three operons icmW-CBU1651-icmX, icmV-dotA-CBU1647 and icmT-icmSicmD-icmC-icmB-CBU1646 showed an increased transcription of these genes during the initial 16 h of C. burnetii infection of a eukaryotic host cell, and a subsequent drop in transcript levels by 24 h post-infection.
Formal Description
Interaction-ID: 32750

process

Coxiella burnetii infection

increases_expression of

gene/protein

icmD

during the initial 16 h of infection
Comment The Dot/Icm type IV secretion system has been analysed in C. burnetii in homology to L. pneumophila. Examination of the relative transcript levels concerning the three operons icmW-CBU1651-icmX, icmV-dotA-CBU1647 and icmT-icmSicmD-icmC-icmB-CBU1646 showed an increased transcription of these genes during the initial 16 h of C. burnetii infection of a eukaryotic host cell, and a subsequent drop in transcript levels by 24 h post-infection.
Formal Description
Interaction-ID: 32752

process

Coxiella burnetii infection

increases_expression of

gene/protein

icmC

during the initial 16 h of infection
Comment The Dot/Icm type IV secretion system has been analysed in C. burnetii in homology to L. pneumophila. Examination of the relative transcript levels concerning the three operons icmW-CBU1651-icmX, icmV-dotA-CBU1647 and icmT-icmSicmD-icmC-icmB-CBU1646 showed an increased transcription of these genes during the initial 16 h of C. burnetii infection of a eukaryotic host cell, and a subsequent drop in transcript levels by 24 h post-infection.
Formal Description
Interaction-ID: 32753

process

Coxiella burnetii infection

increases_expression of

gene/protein

icmB

during the initial 16 h of infection
Comment The Dot/Icm type IV secretion system has been analysed in C. burnetii in homology to L. pneumophila. Examination of the relative transcript levels concerning the three operons icmW-CBU1651-icmX, icmV-dotA-CBU1647 and icmT-icmSicmD-icmC-icmB-CBU1646 showed an increased transcription of these genes during the initial 16 h of C. burnetii infection of a eukaryotic host cell, and a subsequent drop in transcript levels by 24 h post-infection.
Formal Description
Interaction-ID: 32755

process

Coxiella burnetii infection

increases_expression of

gene/protein

CBU_1646

during the initial 16 h of infection
Comment The Dot/Icm type IV secretion system has been analysed in C. burnetii in homology to L. pneumophila. The dot/icm locus is upregulated in response to infection.
Formal Description
Interaction-ID: 32756

process

Coxiella burnetii infection

increases_activity of

process

T4BSS

during the initial 16 h of infection
Comment The Dot/Icm type IV secretion system has been analysed in C. burnetii in homology to L. pneumophila. Examination of the relative transcript levels concerning the three operons icmW-CBU1651-icmX, icmV-dotA-CBU1647 and icmT-icmSicmD-icmC-icmB-CBU1646 showed an increased transcription of these genes during the initial 16 h of C. burnetii infection of a eukaryotic host cell, and a subsequent drop in transcript levels by 24 h post-infection.
Formal Description
Interaction-ID: 32763

gene/protein

icmW

affects_activity of

process

T4BSS

Comment The Dot/Icm type IV secretion system has been analysed in C. burnetii in homology to L. pneumophila. Examination of the relative transcript levels concerning the three operons icmW-CBU1651-icmX, icmV-dotA-CBU1647 and icmT-icmSicmD-icmC-icmB-CBU1646 showed an increased transcription of these genes during the initial 16 h of C. burnetii infection of a eukaryotic host cell, and a subsequent drop in transcript levels by 24 h post-infection.
Formal Description
Interaction-ID: 32765

gene/protein

CBU_1651

affects_activity of

process

T4BSS

Comment The Dot/Icm type IV secretion system has been analysed in C. burnetii in homology to L. pneumophila. Examination of the relative transcript levels concerning the three operons icmW-CBU1651-icmX, icmV-dotA-CBU1647 and icmT-icmSicmD-icmC-icmB-CBU1646 showed an increased transcription of these genes during the initial 16 h of C. burnetii infection of a eukaryotic host cell, and a subsequent drop in transcript levels by 24 h post-infection.
Formal Description
Interaction-ID: 32766

gene/protein

icmX

affects_activity of

process

T4BSS

Comment The Dot/Icm type IV secretion system has been analysed in C. burnetii in homology to L. pneumophila. Examination of the relative transcript levels concerning the three operons icmW-CBU1651-icmX, icmV-dotA-CBU1647 and icmT-icmSicmD-icmC-icmB-CBU1646 showed an increased transcription of these genes during the initial 16 h of C. burnetii infection of a eukaryotic host cell, and a subsequent drop in transcript levels by 24 h post-infection.
Formal Description
Interaction-ID: 32767

gene/protein

icmV

affects_activity of

process

T4BSS

Comment The Dot/Icm type IV secretion system has been analysed in C. burnetii in homology to L. pneumophila. Examination of the relative transcript levels concerning the three operons icmW-CBU1651-icmX, icmV-dotA-CBU1647 and icmT-icmSicmD-icmC-icmB-CBU1646 showed an increased transcription of these genes during the initial 16 h of C. burnetii infection of a eukaryotic host cell, and a subsequent drop in transcript levels by 24 h post-infection.
Formal Description
Interaction-ID: 32768

gene/protein

dotaA

affects_activity of

process

T4BSS

Comment The Dot/Icm type IV secretion system has been analysed in C. burnetii in homology to L. pneumophila. Examination of the relative transcript levels concerning the three operons icmW-CBU1651-icmX, icmV-dotA-CBU1647 and icmT-icmSicmD-icmC-icmB-CBU1646 showed an increased transcription of these genes during the initial 16 h of C. burnetii infection of a eukaryotic host cell, and a subsequent drop in transcript levels by 24 h post-infection.
Formal Description
Interaction-ID: 32769

gene/protein

CBU_1647

affects_activity of

process

T4BSS

Comment The Dot/Icm type IV secretion system has been analysed in C. burnetii in homology to L. pneumophila. Examination of the relative transcript levels concerning the three operons icmW-CBU1651-icmX, icmV-dotA-CBU1647 and icmT-icmSicmD-icmC-icmB-CBU1646 showed an increased transcription of these genes during the initial 16 h of C. burnetii infection of a eukaryotic host cell, and a subsequent drop in transcript levels by 24 h post-infection.
Formal Description
Interaction-ID: 32770

gene/protein

icmT

affects_activity of

process

T4BSS

Comment The Dot/Icm type IV secretion system has been analysed in C. burnetii in homology to L. pneumophila. Examination of the relative transcript levels concerning the three operons icmW-CBU1651-icmX, icmV-dotA-CBU1647 and icmT-icmSicmD-icmC-icmB-CBU1646 showed an increased transcription of these genes during the initial 16 h of C. burnetii infection of a eukaryotic host cell, and a subsequent drop in transcript levels by 24 h post-infection.
Formal Description
Interaction-ID: 32771

gene/protein

icmS

affects_activity of

process

T4BSS

Comment The Dot/Icm type IV secretion system has been analysed in C. burnetii in homology to L. pneumophila. Examination of the relative transcript levels concerning the three operons icmW-CBU1651-icmX, icmV-dotA-CBU1647 and icmT-icmSicmD-icmC-icmB-CBU1646 showed an increased transcription of these genes during the initial 16 h of C. burnetii infection of a eukaryotic host cell, and a subsequent drop in transcript levels by 24 h post-infection.
Formal Description
Interaction-ID: 32773

gene/protein

icmD

affects_activity of

process

T4BSS

Comment The Dot/Icm type IV secretion system has been analysed in C. burnetii in homology to L. pneumophila. Examination of the relative transcript levels concerning the three operons icmW-CBU1651-icmX, icmV-dotA-CBU1647 and icmT-icmSicmD-icmC-icmB-CBU1646 showed an increased transcription of these genes during the initial 16 h of C. burnetii infection of a eukaryotic host cell, and a subsequent drop in transcript levels by 24 h post-infection.
Formal Description
Interaction-ID: 32774

gene/protein

icmC

affects_activity of

process

T4BSS

Comment The Dot/Icm type IV secretion system has been analysed in C. burnetii in homology to L. pneumophila. Examination of the relative transcript levels concerning the three operons icmW-CBU1651-icmX, icmV-dotA-CBU1647 and icmT-icmS-icmD-icmC-icmB-CBU1646 showed an increased transcription of these genes during the initial 16 h of C. burnetii infection of a eukaryotic host cell, and a subsequent drop in transcript levels by 24 h post-infection.
Formal Description
Interaction-ID: 32775

gene/protein

icmB

affects_activity of

process

T4BSS

Comment The Dot/Icm type IV secretion system has been analysed in C. burnetii in homology to L. pneumophila. Examination of the relative transcript levels concerning the three operons icmW-CBU1651-icmX, icmV-dotA-CBU1647 and icmT-icmSicmD-icmC-icmB-CBU1646 showed an increased transcription of these genes during the initial 16 h of C. burnetii infection of a eukaryotic host cell, and a subsequent drop in transcript levels by 24 h post-infection.
Formal Description
Interaction-ID: 32776

gene/protein

CBU_1646

affects_activity of

process

T4BSS

Comment The C. burnetii sigma factor RpoS is abundant in the replicating large cell variants of C. burnetii and undetectable in the stationary small cell variants.
Formal Description
Interaction-ID: 32777

organism

Coxiella burnetii, LCV

increases_quantity of

gene/protein

rpoS

Comment The C. burnetii sigma factor RpoS is abundant in the replicating large cell variants of C. burnetii and undetectable in the stationary small cell variants.
Formal Description
Interaction-ID: 32780

organism

Coxiella burnetii, SCV

NOT increases_quantity of

gene/protein

rpoS

Comment The regulation of the C. burnetii Dot/Icm system is mediated directly by the response regulator PmrA.
Formal Description
Interaction-ID: 32783

gene/protein

pmrA

increases_activity of

process

T4BSS

Comment RT-PCR analyses showed that the dot/icm genes, dotB, icmQ, icmS, and icmW are expressed during C. burnetii infection of Vero cells.
Formal Description
Interaction-ID: 32804

process

Coxiella burnetii infection

increases_expression of

gene/protein

dotB

in Vero cells
Comment RT-PCR analyses showed that the dot/icm genes, dotB, icmQ, icmS, and icmW are expressed during C. burnetii infection of Vero cells.
Formal Description
Interaction-ID: 32805

process

Coxiella burnetii infection

increases_expression of

gene/protein

icmQ

in Vero cells
Comment RT-PCR analyses showed that the dot/icm genes, dotB, icmQ, icmS, and icmW are expressed during C. burnetii infection of Vero cells.
Formal Description
Interaction-ID: 32806

process

Coxiella burnetii infection

increases_expression of

gene/protein

icmS

in Vero cells
Comment RT-PCR analyses showed that the dot/icm genes, dotB, icmQ, icmS, and icmW are expressed during C. burnetii infection of Vero cells.
Formal Description
Interaction-ID: 32807

process

Coxiella burnetii infection

increases_expression of

gene/protein

icmW

in Vero cells
Comment The C. burnetii genes encoding dotB, icmS, icmW, and icmT restored intracellular replication when expressed in a corresponding L. pneumophila deletion strain.
Formal Description
Interaction-ID: 32812

gene/protein

dotB

increases_activity of

process

Coxiella burnetii replication

Comment The C. burnetii genes encoding dotB, icmS, icmW, and icmT restored intracellular replication when expressed in a corresponding L. pneumophila deletion strain.
Formal Description
Interaction-ID: 32813

gene/protein

icmS

increases_activity of

process

Coxiella burnetii replication

Comment The C. burnetii genes encoding dotB, icmS, icmW, and icmT restored intracellular replication when expressed in a corresponding L. pneumophila deletion strain.
Formal Description
Interaction-ID: 32814

gene/protein

icmW

increases_activity of

process

Coxiella burnetii replication

Comment The C. burnetii proteins IcmW and IcmS interact with each other.
Formal Description
Interaction-ID: 32815

gene/protein

icmW

interacts (colocalizes) with

gene/protein

icmS

Comment Chloramphenicol blocks the C. burnetii protein synthesis, preventing the capacity of the CCV (Coxiella containing vacuole) to promote fusion of endocytic vesicles and lysosomes and the formation of the characteristic large spacious vacuole.
Formal Description
Interaction-ID: 32816

drug/chemical compound

Chloramphenicol

decreases_activity of

process

Coxiella burnetii replication

Drugbank entries Show/Hide entries for Chloramphenicol
Comment Chloramphenicol blocks the C. burnetii protein synthesis, preventing the capacity of the CCV (Coxiella containing vacuole) to promote fusion of endocytic vesicles and lysosomes and the formation of the characteristic large spacious vacuole.
Formal Description
Interaction-ID: 32820

drug/chemical compound

Chloramphenicol

decreases_activity of

Drugbank entries Show/Hide entries for Chloramphenicol
Comment Chloramphenicol significantly reduces the capacity of C. burnetii to block the intrinsic death pathway and inhibit host cell apoptosis.
Formal Description
Interaction-ID: 32823

process

Coxiella burnetii infection

decreases_activity of

process

host cell apoptosis

Comment Chloramphenicol significantly reduces the capacity of C. burnetii to block the intrinsic death pathway and inhibit host cell apoptosis.
Formal Description
Interaction-ID: 32825

drug/chemical compound

Chloramphenicol

increases_activity of

process

host cell apoptosis

induced by pathogen
Drugbank entries Show/Hide entries for Chloramphenicol
Comment The application of an icmL::Tn mutant to infect mammalian host cells results in a complete defect in C. burnetii intracellular growth similar to the effect of chloramphenicol on wild type C. burnetii replication in cultured host cells.
Formal Description
Interaction-ID: 32827

gene/protein

icmL.1

increases_activity of

process

Coxiella burnetii replication

Comment The application of an icmL::Tn mutant to infect mammalian host cells results in a complete defect in C. burnetii intracellular growth similar to the effect of chloramphenicol on wild type C. burnetii replication in cultured host cells.
Formal Description
Interaction-ID: 32832

gene/protein

icmL.1

affects_activity of

process

T4BSS

Comment It has been shown by immunoblot analysis that the endogenously-produced effector proteins AnkG and CBU0077 are translocated during C. burnetii infection of host cells.
Formal Description
Interaction-ID: 32834

process

T4BSS

increases_transport of

gene/protein

ankG

Comment It has been shown by immunoblot analysis that the endogenously-produced effector proteins AnkG and CBU0077 are translocated during C. burnetii infection of host cells.
Formal Description
Interaction-ID: 32835

process

T4BSS

increases_transport of

gene/protein

CBU_0077

Comment Concerning the C. burnetii effectors with ankyrin repeats it was shown in a Cya transporter assay that AnkA, AnkB, AnkF and AnkG were translocated in a Dot/Icm-dependent manner.
Formal Description
Interaction-ID: 32838

process

T4BSS

increases_transport of

gene/protein

ankA

shown in a Cya translocation assay
Comment Concerning the C. burnetii effectors with ankyrin repeats it was shown in a Cya transporter assay that AnkA, AnkB, AnkF and AnkG were translocated in a Dot/Icm-dependent manner.
Formal Description
Interaction-ID: 32842

process

T4BSS

increases_transport of

gene/protein

ankB

shown in a Cya translocation assay
Comment Concerning the C. burnetii effectors with ankyrin repeats it was shown in a Cya transporter assay that AnkA, AnkB, AnkF and AnkG were translocated in a Dot/Icm-dependent manner.
Formal Description
Interaction-ID: 32844

process

T4BSS

increases_transport of

gene/protein

ankF

shown in a Cya translocation assay
Comment Concerning the C. burnetii effectors with ankyrin repeats it was shown in a Cya transporter assay that AnkA, AnkB, AnkF and AnkG were translocated in a Dot/Icm-dependent manner.
Formal Description
Interaction-ID: 32845

process

T4BSS

increases_transport of

gene/protein

ankG

shown in a Cya translocation assay
Comment It was shown that efficient Dot/Icm-dependent translocation of AnkA, AnkF, AnkG and AnkP required the chaperone protein IcmS.
Formal Description
Interaction-ID: 32851

gene/protein

icmS

affects transport of

gene/protein

ankA

Comment It was shown that efficient Dot/Icm-dependent translocation of AnkA, AnkF, AnkG and AnkP required the chaperone protein IcmS.
Formal Description
Interaction-ID: 32852

gene/protein

icmS

affects transport of

gene/protein

ankF

Comment It was shown that efficient Dot/Icm-dependent translocation of AnkA, AnkF, AnkG and AnkP required the chaperone protein IcmS.
Formal Description
Interaction-ID: 32853

gene/protein

icmS

affects transport of

gene/protein

ankG

Comment It was shown that efficient Dot/Icm-dependent translocation of AnkA, AnkF, AnkG and AnkP required the chaperone protein IcmS.
Formal Description
Interaction-ID: 32854

gene/protein

icmS

affects transport of

gene/protein

ankP

Comment The effector AnkG, that has been delivered by the Dot/Icm system during infection blocks a cell autonomous host response that promotes cell death and limits pathogen replication.
Formal Description
Interaction-ID: 32858

gene/protein

ankG

decreases_activity of

process

host cell apoptosis

Comment RT-PCR analyses showed that the dot/icm genes, dotB, icmQ, icmS, and icmW are expressed during C. burnetii infection of Vero cells.
Formal Description
Interaction-ID: 35830

gene/protein

icmQ

affects_activity of

process

T4BSS