General Information:

Id: 3,484 (click here to show other Interactions for entry)
Diseases: Q fever
pathogen-host system
Coxiella burnetii/mammalia
THP-1 macrophages, infected with C. burnetii Nine Mile phase II (NMII)
article
Reference: Beare PA et al.(2012) Two Systems for Targeted Gene Deletion in Coxiella burnetii Appl. Environ. Microbiol. 78: 4580-4589 [PMID: 22522687]

Interaction Information:

Comment The C. burnetii proteins dotA and dotB, are probable structural components of the type IVB secretion system (T4BSS). Delta-dotA and delta-dotB mutants failed to secrete T4BSS substrates and to productively infect host cells.
Formal Description
Interaction-ID: 32372

gene/protein

dotaA

increases_activity of

process

T4BSS

analysed in delta-dotA and delta-dotB mutants
Comment The C. burnetii proteins dotA and dotB, are probable structural components of the type IVB secretion system (T4BSS). Delta-dotA and delta-dotB mutants failed to secrete T4BSS substrates and to productively infect host cells.
Formal Description
Interaction-ID: 32375

gene/protein

dotB

increases_activity of

process

T4BSS

analysed in delta-dotA and delta-dotB mutants
Comment The C. burnetii proteins dotA and dotB are required for cytosolic delivery of T4BSS effector proteins CpeD and CpeE.
Formal Description
Interaction-ID: 32376

process

T4BSS

increases_transport of

gene/protein

cpeD

in THP-1 macrophages; shown in a CyaA translocation assay
Comment The C. burnetii proteins dotA and dotB are required for cytosolic delivery of T4BSS effector proteins CpeD and CpeE.
Formal Description
Interaction-ID: 32379

process

T4BSS

increases_transport of

gene/protein

cpeE

in THP-1 macrophages; shown in a CyaA translocation assay
Comment The C. burnetii proteins dotA and dotB are required for cytosolic delivery of T4BSS effector proteins CpeD and CpeE.
Formal Description
Interaction-ID: 32396

gene/protein

dotaA

affects_activity of

process

T4BSS

Comment The C. burnetii proteins dotA and dotB are required for cytosolic delivery of T4BSS effector proteins CpeD and CpeE.
Formal Description
Interaction-ID: 32398

gene/protein

dotB

affects_activity of

process

T4BSS

Comment Delta-dotA and delta-dotB mutants are defective in replication.
Formal Description
Interaction-ID: 32412

process

T4BSS

increases_activity of

process

Coxiella burnetii replication

shown in Vero cells; analysed in delta-dotA and delta-dotB mutants
Comment The lack of intracellular replication by delta-dotA and delta-dotB mutants correlated with failed production of the large and spacious replication vacuole typical of C. burnetii infection.
Formal Description
Interaction-ID: 32414

process

T4BSS

increases_activity of

shown in Vero cells; analysed in delta-dotA and delta-dotB mutants