General Information:

Id: 3,419 (click here to show other Interactions for entry)
Diseases: Pseudomonas aeruginosa diseases
pathogen-host system
Pseudomonas aeruginosa/mammalia
MDCK or Caco-2 cells, infected with P. aeruginosa strain PAO1
article
Reference: Okuda J et al.(2010) Translocation of Pseudomonas aeruginosa from the intestinal tract is mediated by the binding of ExoS to an Na,K-ATPase regulator, FXYD3 Infect. Immun. 78: 4511-4522 [PMID: 20805335]

Interaction Information:

Comment A greater fraction of PAO1 bacteria penetrated the MDCK cell monolayers through the paracellular spaces than by the direct invasion of the cells. The paracellular spaces between epithelial cells are sealed by tight junctions (TJs), which establish the epithelial barrier to prevent bacterial penetration from the intestine into the bloodstream. ExoS is necessary for penetration of P. aeruginosa through epithelial cell monolayers in vitro, the penetration capacity of the delta-exoS strain is strongly attenuated.
Formal Description
Interaction-ID: 31744

gene/protein

exoS

decreases_activity of

in MDCK cell monolayers and Caco-2 colon epithelial cells
Comment A greater fraction of PAO1 bacteria penetrated the MDCK cell monolayers through the paracellular spaces than by the direct invasion of the cells. The paracellular spaces between epithelial cells are sealed by tight junctions (TJs), which establish the epithelial barrier to prevent bacterial penetration from the intestine into the bloodstream. ExoS is necessary for penetration of P. aeruginosa through epithelial cell monolayers in vitro, the penetration capacity of the delta-exoS strain is strongly attenuated.
Formal Description
Interaction-ID: 31766

gene/protein

exoS

decreases_activity of

in MDCK cell monolayers and Caco-2 colon epithelial cells
Comment In a GST pulldown assay it was shown that ExoS binds to FXYD3, a regulator of Na,K-ATPase. A further experiment showed that ExoS was detected only in FXYD3 complexes precipitated from PAO1-infected Caco-2 cell lysates.
Formal Description
Interaction-ID: 31769

gene/protein

exoS

interacts (colocalizes) with

gene/protein

FXYD3

in a GST pulldown assay and Caco-2 cell lysates
Comment ExoS inactivates Na,K-ATPase activity through interactions with FXYD3. Membranes isolated from control Caco-2 cells exhibited Na,K-ATPase activity, whereas membranes isolated from FXYD3 knockdown cells did not. Exposure of control membranes to recombinant ExoS did not show Na,K-ATPase activity.
Formal Description
Interaction-ID: 31789

gene/protein

exoS

decreases_activity of

in Caco-2 cells
Comment ExoS inactivates Na,K-ATPase activity through interactions with FXYD3. Membranes isolated from control Caco-2 cells exhibited Na,K-ATPase activity, whereas membranes isolated from FXYD3 knockdown cells did not. Exposure of control membranes to recombinant ExoS did not show Na,K-ATPase activity.
Formal Description
Interaction-ID: 31791

gene/protein

exoS

decreases_activity of

gene/protein

FXYD3

in Caco-2 cells
Comment Western blot analysis and confocal imaging revealed that infection of Caco-2 cells with the wild-type or delta-exoS/exoS strain reduced the levels of expression of ZO-1 and occludin leading to disruption of the tight junction structure. The infection with the delta-exoS strain was used as control.
Formal Description
Interaction-ID: 31793

gene/protein

exoS

decreases_quantity of

gene/protein

OCLN

in Caco-2 cells
Comment Western blot analysis and confocal imaging revealed that infection of Caco-2 cells with the wild-type or delta-exoS/exoS strain reduced the levels of expression of ZO-1 and occludin leading to disruption of the tight junction structure. The infection with the delta-exoS strain was used as control.
Formal Description
Interaction-ID: 31800

gene/protein

exoS

decreases_quantity of

gene/protein

TJP1

in Caco-2 cells