General Information:

Id: 3,415 (click here to show other Interactions for entry)
Diseases: Pseudomonas aeruginosa diseases
pathogen-host system
Pseudomonas aeruginosa/mammalia
BTO:0000195 CACO-2 cell monolayers, infected with P. aeruginosa strain PAO1
article
Reference: Okuda J et al.(2011) Degradation of interleukin 8 by the serine protease MucD of Pseudomonas aeruginosa J. Infect. Chemother. 17: 782-792 [PMID: 21626303]

Interaction Information:

Comment There was a fourfold increase in the level of of IL-8, a chemotactic factor that attracts neutrophils, in the culture medium of Caco-2 cells infected with delta-exoS relative to the level in the medium of cells infected with the wild-type strain. These results were verified in Caco-2 cell monolayers. The IL-8 concentration in the culture medium, infected with delta-exoS was significantly higher at the apical side but not at the basolateral side of the Caco-2 cell monolaye.
Formal Description
Interaction-ID: 31692

gene/protein

exoS

decreases_quantity of

gene/protein

CXCL8

in the culture medium of Caco-2 monolayers
Comment The level of the Il-8 mRNA in cells infected with the wild-type strain was 3.2-fold greater than that observed in cells infected with delta-exoS, showing inconsistence with the analysis of the IL-8 protei.
Formal Description
Interaction-ID: 31700

gene/protein

exoS

increases_expression of

gene/protein

CXCL8

in the culture medium of Caco-2 monolayers
Comment The experiments suggest that the serine protease MucD, which is regulated by ExoS, directly or indirectly degrades IL-8 so that IL-8-dependent neutrophil infiltration and successive bacterial killing are attenuated.
Formal Description
Interaction-ID: 31705

gene/protein

exoS

affects_activity of

gene/protein

mucD