General Information:

Id: 3,379
Diseases: Pseudomonas aeruginosa diseases
pathogen-host system
Pseudomonas aeruginosa/mammalia
PMN (polymorphonuclear neutrophils) of different donors, infected with P. aeruginosa strain PAO1
article
Reference: Kahle NA et al.(2013) Bacterial quorum sensing molecule induces chemotaxis of human neutrophils via induction of p38 and leukocyte specific protein 1 (LSP1) Immunobiology 218: 145-151 [PMID: 22401915]

Interaction Information:

Comment AHL-12 (N-(3-oxododecanoyl)-l-homoserine lactone) induced chemotaxis of neutrophils.
Formal Description
Interaction-ID: 31446

drug/chemical compound

N-(3-Oxododecanoyl)-HSL

increases_activity of

Comment SB203580, an inhibitor of p38 MAPK, prevented the AHL-12 (N-(3-oxododecanoyl)-l-homoserine lactone) induced chemotaxis.
Formal Description
Interaction-ID: 31451

drug/chemical compound

SB203580

decreases_activity of

gene/protein

p38 MAPK

induced by AHL-12
Comment p38 MAPK was phosphorylated within minutes in response to AHL-12 (N-(3-oxododecanoyl)-l-homoserine lactone), as was its downstream target, the MAPKAP-Kinase-2 (MK2).
Formal Description
Interaction-ID: 31452

drug/chemical compound

N-(3-Oxododecanoyl)-HSL

increases_phosphorylation of

gene/protein

p38 MAPK

Comment p38 MAPK was phosphorylated within minutes in response to AHL-12 (N-(3-oxododecanoyl)-l-homoserine lactone), as was its downstream target, the MAPKAP-Kinase-2 (MK2).
Formal Description
Interaction-ID: 31454

drug/chemical compound

N-(3-Oxododecanoyl)-HSL

increases_activity of

gene/protein

p38 MAPK

Comment p38 MAPK was phosphorylated within minutes in response to AHL-12 (N-(3-oxododecanoyl)-l-homoserine lactone), as was its downstream target, the MAPKAP-Kinase-2 (MK2).
Formal Description
Interaction-ID: 31455

gene/protein

p38 MAPK

increases_phosphorylation of

gene/protein

MAPKAPK2

Drugbank entries Show/Hide entries for MAPKAPK2
Comment p38 MAPK was phosphorylated within minutes in response to AHL-12 (N-(3-oxododecanoyl)-l-homoserine lactone), as was its downstream target, the MAPKAP-Kinase-2 (MK2).
Formal Description
Interaction-ID: 31456

gene/protein

p38 MAPK

increases_activity of

gene/protein

MAPKAPK2

Drugbank entries Show/Hide entries for MAPKAPK2
Comment The major substrate of MK2 in PMN is the leukocyte specific protein 1 (LSP1). LSP1 binds to F-actin and participates directly in actin polymerization and cell migration. In response to AHL-12, LSP1 was phosphorylated and co-localized with F-actin in polarized PMN.
Formal Description
Interaction-ID: 31458

gene/protein

MAPKAPK2

increases_phosphorylation of

gene/protein

LSP1

Drugbank entries Show/Hide entries for MAPKAPK2
Comment AHL-12 induced a shape change of the PMN, actin polymerization and capping of vinculin.
Formal Description
Interaction-ID: 31459

drug/chemical compound

N-(3-Oxododecanoyl)-HSL

affects_activity of

in PMN (polymorphonuclear neutrophils)
Comment SB203580, an inhibitor of p38 MAPK, prevented the AHL-12 (N-(3-oxododecanoyl)-l-homoserine lactone) induced chemotaxis.
Formal Description
Interaction-ID: 31604

gene/protein

p38 MAPK

increases_activity of

induced by AHL-12
Comment The major substrate of MK2 in PMN is the leukocyte specific protein 1 (LSP1). LSP1 binds to F-actin and participates directly in actin polymerization and cell migration. In response to AHL-12, LSP1 was phosphorylated and co-localized with F-actin in polarized PMN.
Formal Description
Interaction-ID: 31609

gene/protein

MAPKAPK2

increases_activity of

gene/protein

LSP1

Drugbank entries Show/Hide entries for MAPKAPK2
Comment The major substrate of MK2 in PMN is the leukocyte specific protein 1 (LSP1). LSP1 binds to F-actin and participates directly in actin polymerization and cell migration. In response to AHL-12, LSP1 was phosphorylated and co-localized with F-actin in polarized PMN.
Formal Description
Interaction-ID: 31611

gene/protein

LSP1

affects_activity of

induced by AHL-12