General Information:

Id: 3,156
Diseases: Pseudomonas aeruginosa diseases
pathogen-host system
Pseudomonas aeruginosa/mammalia
P. aeruginosa strain H103, mutant H636, H636O
article
Reference: Fito-Boncompte L et al.(2011) Full virulence of Pseudomonas aeruginosa requires OprF. Infect. Immun. 79: 1176-1186 [PMID: 21189321]

Interaction Information:

Comment OprF is required for bacterial adhesion onto eukaryotic cells.
Formal Description
Interaction-ID: 29036

gene/protein

oprF

increases_activity of

as shown with rat glial cells and Caco-2/TC7cells, infected with P. aeruginosa strain H103 and mutants
Comment The oprF mutant failed to secrete ExoS and ExoT, even under calcium-depleted conditions, showing that T3SS activity is impaired in the absence of OprF.
Formal Description
Interaction-ID: 29037

gene/protein

oprF

increases_activity of

process

T3SS

Comment The oprF mutant failed to secrete ExoS and ExoT, even under calcium-depleted conditions, showing that T3SS activity is impaired in the absence of OprF.
Formal Description
Interaction-ID: 29038

gene/protein

oprF

increases_transport of

gene/protein

exoS

Comment The oprF mutant failed to secrete ExoS and ExoT, even under calcium-depleted conditions, showing that T3SS activity is impaired in the absence of OprF.
Formal Description
Interaction-ID: 29039

gene/protein

oprF

increases_transport of

gene/protein

exoT

Comment In agreement with the reduced virulence phenotype of the oprF mutant the major virulence factors, pyocyanin, exotoxin A, lectin PA-1L, and elastase are strongly affected by the inactivation of oprF. Pyocyanin production by the different strains revealed that this phenazine is not detected in the oprF mutant supernatant grown to the entry of the stationary phase and is strongly reduced when it is grown to the stationary phase.
Formal Description
Interaction-ID: 29040

gene/protein

oprF

increases_quantity of

drug/chemical compound

Pyocyanine

during the entry into the stationary phase
Comment In agreement with the reduced virulence phenotype of the oprF mutant the major virulence factors, pyocyanin, exotoxin A, lectin PA-1L, and elastase are strongly affected by the inactivation of oprF. Exotoxin A production was obtained during the entry into the stationary growth phase in the wild-type strain but was strongly decreased in the oprF mutant. The oprF complementation partially restored this phenotype.
Formal Description
Interaction-ID: 29041

gene/protein

oprF

increases_quantity of

gene/protein

toxA

during the entry into the stationary phase
Drugbank entries Show/Hide entries for toxA
Comment In agreement with the reduced virulence phenotype of the oprF mutant the major virulence factors, pyocyanin, exotoxin A, lectin PA-1L, and elastase are strongly affected by the inactivation of oprF. The expression of lecA, encoding the lectin PA-1L, was strongly reduced in the oprF mutant and was fully restored in the oprF-complemented strain.
Formal Description
Interaction-ID: 29042

gene/protein

oprF

increases_expression of

gene/protein

lecA

Comment Pyocyanin, exotoxin A, lectin PA-1L, and elastase are strongly affected by the inactivation of oprF. When the cells were grown to the entry into the stationary phase, a significant reduction of the elastase amount secreted by oprF mutant H636 was observed. If grown to stationary phase, the elastolytic activity of the oprF mutant strain was found to be similar to the activities of the two other strains, indicating that the production of elastase is delayed but not abrogated.
Formal Description
Interaction-ID: 29043

gene/protein

oprF

increases_quantity of

gene/protein

lasB

during the entry into the stationary phase
Drugbank entries Show/Hide entries for lasB
Comment In the oprF mutant, production of the signal molecules N-(3-oxododecanoyl)-l-homoserine lactone and N-butanoyl-l-homoserine lactone was found to be reduced and delayed, respectively.
Formal Description
Interaction-ID: 29044

gene/protein

oprF

increases_quantity of

drug/chemical compound

N-(3-Oxododecanoyl)-HSL

Comment In the oprF mutant, production of the signal molecules N-(3-oxododecanoyl)-l-homoserine lactone and N-butanoyl-l-homoserine lactone was found to be reduced and delayed, respectively.
Formal Description
Interaction-ID: 29045

gene/protein

oprF

affects_quantity of

drug/chemical compound

N-Butyryl-HSL

Comment In the oprF mutant, Pseudomonas quinolone signal (PQS) production was decreased, while its precursor, 4-hydroxy-2-heptylquinoline (HHQ), accumulated in the cells.
Formal Description
Interaction-ID: 29047

gene/protein

oprF

increases_quantity of

drug/chemical compound

2-Heptyl-3-hydroxy-4-quinolone, PQS

Comment In the oprF mutant, Pseudomonas quinolone signal (PQS) production was decreased, while its precursor, 4-hydroxy-2-heptylquinoline (HHQ), accumulated in the cells.
Formal Description
Interaction-ID: 29049

gene/protein

oprF

affects_quantity of

drug/chemical compound

2-Heptyl-4-quinolone, HHQ