General Information:

Id: 3,140 (click here to show other Interactions for entry)
Diseases: Pseudomonas aeruginosa diseases
pathogen-host system
Pseudomonas aeruginosa/mammalia
review
Reference: Engel J and Eran Y(2011) Subversion of mucosal barrier polarity by pseudomonas aeruginosa. Front Microbiol 2: 114 [PMID: 21747810]

Interaction Information:

Comment The mucosal barrier epithelium is comprised of one or more layers of epithelial cells that have specialized and distinct apical and basolateral surfaces, separated by tight junctions (TJs), that form selective permeability barriers between biological compartments. For opportunistic pathogens, like P. aeruginosa, the mucosal barrier represents a formidable challenge to bacterial-mediated damage or entry.
Formal Description
Interaction-ID: 28915

process

P. aeruginosa infection

decreases_activity of

of mucosal surface
Comment Studies of the interaction of P. aeruginosa with polarized epithelium in culture and in vivo show that the degree of polarity significantly affects the final outcome of infection. P. aeruginosa preferentially adheres to, enters, and injures wounded epithelium.
Formal Description
Interaction-ID: 28917

process

P. aeruginosa infection

decreases_activity of

Comment At the apical surface N-glycan chains were necessary and sufficient for P. aeruginosa binding, invasion, and cytotoxicity to MDCK and Calu-3 cells grown at various states of polarization.
Formal Description
Interaction-ID: 28918

cellular component

N-glycan

increases_activity of

process

P. aeruginosa infection

at the apical surface
Comment At the basolateral surface, the sulfation of heparan sulfate (HS) chains of HSPGs (heparan sulfate proteoglycans) was found to be critical for P. aeruginosa binding, cytotoxicity, and invasion. In incompletely polarized epithelium, HSPG abundance was increased at the apical surface, explaining at least in part the increased susceptibility of injured epithelium to P. aeruginosa colonization and damage.
Formal Description
Interaction-ID: 28919

cellular component

heparan sulfate proteoglycans, HSPGs

increases_activity of

process

P. aeruginosa infection

at the basolateral surface
Comment P. aeruginosa binding activates a central host signaling molecule, phosphatidylinositol 3-kinase (PI3K), which is required for the synthesis of PIP3 and for activation of a downstream effector, the serine/threonine kinase Akt.
Formal Description
Interaction-ID: 28920

process

P. aeruginosa infection

affects_activity of

Comment P. aeruginosa transforms apical into basolateral membrane, creating a local microenvironment that facilitates its colonization and entry into to the mucosal barrier. In polarized monolayers, P. aeruginosa binds near cell-cell junctions, where it activates and recruits PI3K to the apical surface, leading to a remarkable remodeling of the apical membrane in which protrusions enriched for PIP3 and actin form at the apical surface at the site of bacterial binding.
Formal Description
Interaction-ID: 28922

process

P. aeruginosa infection

increases_activity of

protrusions are deficient in apical markers and contain basolateral markers