General Information:

Id: 3,130
Diseases: Pseudomonas aeruginosa diseases
pathogen-host system
Pseudomonas aeruginosa/mammalia
review
Reference: Tashiro Y et al.(2012) Multifunctional membrane vesicles in Pseudomonas aeruginosa Environ. Microbiol. 14: 1349-1362 [PMID: 22103313]

Interaction Information:

Comment The following representative proteins are associated with Pseudomonas aeruginosa MVs (membrane vesicles). The periplasmic proteins are alkaline phosphatase PhoA, beta-lactamase and peptidoglycan (PG) hydrolase.
Formal Description
Interaction-ID: 28800

gene/protein

phoA

is localized in

cellular component

bacterial membrane vesicle, MV

Drugbank entries Show/Hide entries for phoA
Comment The following representative proteins are associated with Pseudomonas aeruginosa MVs (membrane vesicles). The periplasmic proteins are alkaline phosphatase PhoA, beta-lactamase and peptidoglycan (PG) hydrolase.
Formal Description
Interaction-ID: 28806

gene/protein

Beta-lactamase

is localized in

cellular component

bacterial membrane vesicle, MV

Comment The following representative proteins are associated with Pseudomonas aeruginosa MVs (membrane vesicles). The periplasmic proteins are alkaline phosphatase PhoA, beta-lactamase and peptidoglycan (PG) hydrolase.
Formal Description
Interaction-ID: 28807

gene/protein

flgJ

is localized in

cellular component

bacterial membrane vesicle, MV

Comment The following representative proteins are associated with Pseudomonas aeruginosa MVs (membrane vesicles).The MVs were found to contain elastase (LasB) precursors.
Formal Description
Interaction-ID: 28810

gene/protein

lasB

is localized in

cellular component

bacterial membrane vesicle, MV

Drugbank entries Show/Hide entries for lasB
Comment The following representative proteins are associated with Pseudomonas aeruginosa MVs (membrane vesicles). Extracellular virulence factors are phospholipase C (PlcB), hemolytic phospholipase (PlcH), CFTR inhibitory factor Cif and leucine aminopeptidase PaAP.
Formal Description
Interaction-ID: 28811

gene/protein

plcB

is localized in

cellular component

bacterial membrane vesicle, MV

Comment The following representative proteins are associated with Pseudomonas aeruginosa MVs (membrane vesicles). Extracellular virulence factors are phospholipase C (PlcB), hemolytic phospholipase (PlcH), CFTR inhibitory factor Cif and leucine aminopeptidase PaAP.
Formal Description
Interaction-ID: 28812

gene/protein

plcH

is localized in

cellular component

bacterial membrane vesicle, MV

Comment The following representative proteins are associated with Pseudomonas aeruginosa MVs (membrane vesicles). Extracellular virulence factors are phospholipase C (PlcB), hemolytic phospholipase (PlcH), CFTR inhibitory factor Cif and leucine aminopeptidase PaAP.
Formal Description
Interaction-ID: 28813

gene/protein

cif

is localized in

cellular component

bacterial membrane vesicle, MV

Comment The following representative proteins are associated with Pseudomonas aeruginosa MVs (membrane vesicles). Extracellular virulence factors are phospholipase C (PlcB), hemolytic phospholipase (PlcH), CFTR inhibitory factor Cif and leucine aminopeptidase PaAP.
Formal Description
Interaction-ID: 28814

gene/protein

paaP

is localized in

cellular component

bacterial membrane vesicle, MV

Comment The following representative proteins are associated with Pseudomonas aeruginosa MVs (membrane vesicles). The inner membrane protein is multidrug efflux membrane protein MexA.
Formal Description
Interaction-ID: 28815

gene/protein

mexA

is localized in

cellular component

bacterial membrane vesicle, MV

Drugbank entries Show/Hide entries for mexA
Comment The following representative proteins are associated with Pseudomonas aeruginosa MVs (membrane vesicles). The major OMPs of the MVs fraction have been identified as OprD, OprE, OprF, OprG, OprH, OprI, PagL and PcoB.
Formal Description
Interaction-ID: 28816

gene/protein

oprD

is localized in

cellular component

bacterial membrane vesicle, MV

Drugbank entries Show/Hide entries for oprD
Comment The following representative proteins are associated with Pseudomonas aeruginosa MVs (membrane vesicles). The major OMPs of the MVs fraction have been identified as OprD, OprE, OprF, OprG, OprH, OprI, PagL and PcoB.
Formal Description
Interaction-ID: 28818

gene/protein

oprE

is localized in

cellular component

bacterial membrane vesicle, MV

Comment The following representative proteins are associated with Pseudomonas aeruginosa MVs (membrane vesicles). The major OMPs of the MVs fraction have been identified as OprD, OprE, OprF, OprG, OprH, OprI, PagL and PcoB.
Formal Description
Interaction-ID: 28819

gene/protein

oprF

is localized in

cellular component

bacterial membrane vesicle, MV

Comment The following representative proteins are associated with Pseudomonas aeruginosa MVs (membrane vesicles). The major OMPs of the MVs fraction have been identified as OprD, OprE, OprF, OprG, OprH, OprI, PagL and PcoB.
Formal Description
Interaction-ID: 28820

gene/protein

oprG

is localized in

cellular component

bacterial membrane vesicle, MV

Comment The following representative proteins are associated with Pseudomonas aeruginosa MVs (membrane vesicles). The major OMPs of the MVs fraction have been identified as OprD, OprE, OprF, OprG, OprH, OprI, PagL and PcoB.
Formal Description
Interaction-ID: 28821

gene/protein

oprH

is localized in

cellular component

bacterial membrane vesicle, MV

Comment The following representative proteins are associated with Pseudomonas aeruginosa MVs (membrane vesicles). The major OMPs of the MVs fraction have been identified as OprD, OprE, OprF, OprG, OprH, OprI, PagL and PcoB.
Formal Description
Interaction-ID: 28822

gene/protein

oprI

is localized in

cellular component

bacterial membrane vesicle, MV

Comment The following representative proteins are associated with Pseudomonas aeruginosa MVs (membrane vesicles). The major OMPs of the MVs fraction have been identified as OprD, OprE, OprF, OprG, OprH, OprI, PagL and PcoB.
Formal Description
Interaction-ID: 28823

gene/protein

pagL

is localized in

cellular component

bacterial membrane vesicle, MV

Comment The following representative proteins are associated with Pseudomonas aeruginosa MVs (membrane vesicles). The major OMPs of the MVs fraction have been identified as OprD, OprE, OprF, OprG, OprH, OprI, PagL and PcoB.
Formal Description
Interaction-ID: 28824

gene/protein

pcoB

is localized in

cellular component

bacterial membrane vesicle, MV

Comment The following representative proteins are associated with Pseudomonas aeruginosa MVs (membrane vesicles). Proteins concerning the appendixes are flagellin type B (FliC) and fimbrial protein PilA.
Formal Description
Interaction-ID: 28825

gene/protein

fliC

is localized in

cellular component

bacterial membrane vesicle, MV

Comment The following representative proteins are associated with Pseudomonas aeruginosa MVs (membrane vesicles). Proteins concerning the appendixes are flagellin type B (FliC) and fimbrial protein PilA.
Formal Description
Interaction-ID: 28827

gene/protein

pilA

is localized in

cellular component

bacterial membrane vesicle, MV

Comment The following representative proteins are associated with Pseudomonas aeruginosa MVs (membrane vesicles). A proteomic analysis of P. aeruginosa has shown that naturally secreted MVs also contain cytoplasmic proteins, including 50S ribosomal proteins, ATP synthetase subunits and cytochrome c oxidases.
Formal Description
Interaction-ID: 28828

gene/protein

rplT

is localized in

cellular component

bacterial membrane vesicle, MV

Comment The following representative proteins are associated with Pseudomonas aeruginosa MVs (membrane vesicles). A proteomic analysis of P. aeruginosa has shown that naturally secreted MVs also contain cytoplasmic proteins, including 50S ribosomal proteins, ATP synthetase subunits and cytochrome c oxidases.
Formal Description
Interaction-ID: 28829

gene/protein

rplU

is localized in

cellular component

bacterial membrane vesicle, MV

Comment The following representative proteins are associated with Pseudomonas aeruginosa MVs (membrane vesicles). A proteomic analysis of P. aeruginosa has shown that naturally secreted MVs also contain cytoplasmic proteins, including 50S ribosomal proteins, ATP synthetase subunits and cytochrome c oxidases.
Formal Description
Interaction-ID: 28830

gene/protein

atpB

is localized in

cellular component

bacterial membrane vesicle, MV

Comment The following representative proteins are associated with Pseudomonas aeruginosa MVs (membrane vesicles). A proteomic analysis of P. aeruginosa has shown that naturally secreted MVs also contain cytoplasmic proteins, including 50S ribosomal proteins, ATP synthetase subunits and cytochrome c oxidases.
Formal Description
Interaction-ID: 28831

gene/protein

ccoP1

is localized in

cellular component

bacterial membrane vesicle, MV

Comment The following representative proteins are associated with Pseudomonas aeruginosa MVs (membrane vesicles). A proteomic analysis of P. aeruginosa has shown that naturally secreted MVs also contain cytoplasmic proteins, including 50S ribosomal proteins, ATP synthetase subunits and cytochrome c oxidases.
Formal Description
Interaction-ID: 28833

gene/protein

ccoP2

is localized in

cellular component

bacterial membrane vesicle, MV

Comment PQS (Pseudomonas quinolone signal) is not only packaged in MVs but also strongly affects MV formation.
Formal Description
Interaction-ID: 28834

drug/chemical compound

2-Heptyl-3-hydroxy-4-quinolone, PQS

is localized in

cellular component

bacterial membrane vesicle, MV

Comment PQS (Pseudomonas quinolone signal) is not only packaged in MVs but also strongly affects MV formation.
Formal Description
Interaction-ID: 28835

drug/chemical compound

2-Heptyl-3-hydroxy-4-quinolone, PQS

increases_activity of

process

bacterial membrane budding

Comment PQS is extremely hydrophobic compared with the two HSLs (N-acylhomoserine lactones) and rarely freely diffuses out of bacterial cells. In P. aeruginosa, MVs containing PQS can serve as a shuttle for this compound.
Formal Description
Interaction-ID: 28837

cellular component

bacterial membrane vesicle, MV

affects transport of

drug/chemical compound

2-Heptyl-3-hydroxy-4-quinolone, PQS

Comment MVs likely enable P. aeruginosa to maintain a competitive advantage when growing in polymicrobial communities.
Formal Description
Interaction-ID: 28838

cellular component

bacterial membrane vesicle, MV

affects_activity of

process

killing of other bacteria

Comment MVs are a major component of the matrix of P. aeruginosa biofilms. The ability to produce MVs is stronger in biofilm cells than in planktonic cells, biofilms contain more LPS per unit weight than do their planktonic counterparts, and biofilm MVs clearly represent a major reservoir of LPS within the biofilm matrix.
Formal Description
Interaction-ID: 28839

increases_quantity of

cellular component

bacterial membrane vesicle, MV

Comment Pseudomonas aeruginosa MVs contain extracellular DNA, both inside the MV and on its surface, and MV surface-bound DNA in biofilms appears to be an electrostatic bridging component that is central to the interaction among MVs, bacterial cells and other biofilm matrix components.
Formal Description
Interaction-ID: 28840

cellular component

extracellular DNA, eDNA

is localized in

cellular component

bacterial membrane vesicle, MV

both inside the MV and on its surface
Comment The aminopeptidase PaAP, which is a major protein contained in MVs, plays an important role in the effective association between P. aeruginosa MVs and host.
Formal Description
Interaction-ID: 28841

gene/protein

paaP

increases_activity of

process

bacterial membrane vesicle fusion with host cell membrane

Comment In most bacteria, the major glycerophospholipid is phosphatidylethanolamine as shown for P. aeruginosa as well. The major glycerophospholipid in P. aeruginosa MVs is phosphatidylglycerol.
Formal Description
Interaction-ID: 28843

drug/chemical compound

Phosphatidylglycerol

is localized in

cellular component

bacterial membrane vesicle, MV

as major component
Comment The composition ratio of fatty acids is quite different between the cellular OM (outer membrane) and MVs (membrane vesicles). While the content ratio of stearic acid (C18:0) is very low in the fatty acids of cellular OMs, this phospholipid is a major component of MVs.
Formal Description
Interaction-ID: 28852

drug/chemical compound

Stearic acid

is localized in

cellular component

bacterial membrane vesicle, MV

as major component
Drugbank entries Show/Hide entries for Stearic acid
Comment High levels of B-band LPS have been detected in P. aeruginosa PAO1 MVs during normal growth conditions, while A-band LPS is a minor component.
Formal Description
Interaction-ID: 28854

drug/chemical compound

Lipopolysaccharide B-band

is localized in

cellular component

bacterial membrane vesicle, MV

as major component