General Information:

Id: 3,089
Diseases: Pseudomonas aeruginosa diseases
pathogen-host system
Pseudomonas aeruginosa/mammalia
P. aeruginosa strain PA14
article
Reference: Lesic B et al.(2009) Quorum sensing differentially regulates Pseudomonas aeruginosa type VI secretion locus I and homologous loci II and III, which are required for pathogenesis. Microbiology (Reading 155: 2845-2855 [PMID: 19497948]

Interaction Information:

Comment MvfR and LasR positively regulate hcp2 and hcp3 and negatively regulate hcp1. The results demonstrate that the expression of all three PA14 T6S loci was affected by QS, although the regulation may not be due to direct binding of LasR or MvfR. Both the LasR and MvfR systems negatively affect HSI-I transcription and positively affect transcription of HSI-II and HSI-III.
Formal Description
Interaction-ID: 28351

gene/protein

hcp2

is_part_of

complex/PPI

T6SS-HSI-2 complex

Comment MvfR and LasR positively regulate hcp2 and hcp3 and negatively regulate hcp1. The results demonstrate that the expression of all three PA14 T6S loci was affected by QS, although the regulation may not be due to direct binding of LasR or MvfR. Both the LasR and MvfR systems negatively affect HSI-I transcription and positively affect transcription of HSI-II and HSI-III.
Formal Description
Interaction-ID: 28354

gene/protein

mvfR

increases_expression of

complex/PPI

T6SS-HSI-2 complex

shown in a microarray and qRT-PCR approach
Comment MvfR and LasR positively regulate hcp2 and hcp3 and negatively regulate hcp1. The results demonstrate that the expression of all three PA14 T6S loci was affected by QS, although the regulation may not be due to direct binding of LasR or MvfR. Both the LasR and MvfR systems negatively affect HSI-I transcription and positively affect transcription of HSI-II and HSI-III.
Formal Description
Interaction-ID: 28355

process

LasR/I-3OC12-(HSL)-system

increases_expression of

complex/PPI

T6SS-HSI-2 complex

shown in a microarray and qRT-PCR approach
Comment MvfR and LasR positively regulate hcp2 and hcp3 and negatively regulate hcp1. The results demonstrate that the expression of all three PA14 T6S loci was affected by QS, although the regulation may not be due to direct binding of LasR or MvfR. Both the LasR and MvfR systems negatively affect HSI-I transcription and positively affect transcription of HSI-II and HSI-III.
Formal Description
Interaction-ID: 28356

gene/protein

hcp3

is_part_of

complex/PPI

T6SS-HSI-3, right operon

Comment MvfR and LasR positively regulate hcp2 and hcp3 and negatively regulate hcp1. The results demonstrate that the expression of all three PA14 T6S loci was affected by QS, although the regulation may not be due to direct binding of LasR or MvfR. Both the LasR and MvfR systems negatively affect HSI-I transcription and positively affect transcription of HSI-II and HSI-III.
Formal Description
Interaction-ID: 28357

gene/protein

mvfR

increases_expression of

complex/PPI

T6SS-HSI-3 complex

shown in a microarray and qRT-PCR approach
Comment MvfR and LasR positively regulate hcp2 and hcp3 and negatively regulate hcp1. The results demonstrate that the expression of all three PA14 T6S loci was affected by QS, although the regulation may not be due to direct binding of LasR or MvfR. Both the LasR and MvfR systems negatively affect HSI-I transcription and positively affect transcription of HSI-II and HSI-III.
Formal Description
Interaction-ID: 28358

process

LasR/I-3OC12-(HSL)-system

increases_expression of

complex/PPI

T6SS-HSI-3 complex

shown in a microarray and qRT-PCR approach
Comment MvfR and LasR positively regulate hcp2 and hcp3 and negatively regulate hcp1. The results demonstrate that the expression of all three PA14 T6S loci was affected by QS, although the regulation may not be due to direct binding of LasR or MvfR. Both the LasR and MvfR systems negatively affect HSI-I transcription and positively affect transcription of HSI-II and HSI-III.
Formal Description
Interaction-ID: 28359

gene/protein

hcp1

is_part_of

complex/PPI

T6SS-HSI-1 complex

Comment MvfR and LasR positively regulate hcp2 and hcp3 and negatively regulate hcp1. The results demonstrate that the expression of all three PA14 T6S loci was affected by QS, although the regulation may not be due to direct binding of LasR or MvfR. Both the LasR and MvfR systems negatively affect HSI-I transcription and positively affect transcription of HSI-II and HSI-III.
Formal Description
Interaction-ID: 28360

gene/protein

mvfR

decreases_expression of

complex/PPI

T6SS-HSI-1 complex

shown in a microarray and qRT-PCR approach
Comment MvfR and LasR positively regulate hcp2 and hcp3 and negatively regulate hcp1. The results demonstrate that the expression of all three PA14 T6S loci was affected by QS, although the regulation may not be due to direct binding of LasR or MvfR. Both the LasR and MvfR systems negatively affect HSI-I transcription and positively affect transcription of HSI-II and HSI-III.
Formal Description
Interaction-ID: 28361

process

LasR/I-3OC12-(HSL)-system

decreases_expression of

complex/PPI

T6SS-HSI-1 complex

shown in a microarray and qRT-PCR approach
Comment HSI-II and HSI-III play important roles in P. aeruginosa virulence, as shown in three different infection models (Arabidopsis, acute mouse lung and acute mouse burn infection).
Formal Description
Interaction-ID: 28362

process

P. aeruginosa infection, acute

increases_activity of

process

T6SS-HSI-2

shown in Arabidopsis, acute mouse lung and acute mouse burn infection models
Comment HSI-II and HSI-III play important roles in P. aeruginosa virulence, as shown in three different infection models (Arabidopsis, acute mouse lung and acute mouse burn infection).
Formal Description
Interaction-ID: 28363

process

P. aeruginosa infection, acute

increases_activity of

process

T6SS-HSI-3

shown in Arabidopsis, acute mouse lung and acute mouse burn infection models
Comment The mvfR inhibitors 4CABA (2-amino-4-chlorobenzoic acid) and 6CABA (2-amino-6-chlorobenzoic acid) repressed transcription of about half of the HSI-II-associated genes and almost all of the HSI-III-associated genes. The downregulation of hcp2 and hcp3 transcription by 4CABA and 6CABA was further confirmed by qRT-PCR.
Formal Description
Interaction-ID: 28364

drug/chemical compound

4CABA

decreases_expression of

complex/PPI

T6SS-HSI-2 complex

shown in a microarray and qRT-PCR approach
Comment The mvfR inhibitors 4CABA (2-amino-4-chlorobenzoic acid) and 6CABA (2-amino-6-chlorobenzoic acid) repressed transcription of about half of the HSI-II-associated genes and almost all of the HSI-III-associated genes. The downregulation of hcp2 and hcp3 transcription by 4CABA and 6CABA was further confirmed by qRT-PCR.
Formal Description
Interaction-ID: 28365

drug/chemical compound

4CABA

decreases_expression of

complex/PPI

T6SS-HSI-3 complex

shown in a microarray and qRT-PCR approach
Comment The mvfR inhibitors 4CABA (2-amino-4-chlorobenzoic acid) and 6CABA (2-amino-6-chlorobenzoic acid) repressed transcription of about half of the HSI-II-associated genes and almost all of the HSI-III-associated genes. The downregulation of hcp2 and hcp3 transcription by 4CABA and 6CABA was further confirmed by qRT-PCR.
Formal Description
Interaction-ID: 28366

drug/chemical compound

6CABA

decreases_expression of

complex/PPI

T6SS-HSI-2 complex

shown in a microarray and qRT-PCR approach
Comment The mvfR inhibitors 4CABA (2-amino-4-chlorobenzoic acid) and 6CABA (2-amino-6-chlorobenzoic acid) repressed transcription of about half of the HSI-II-associated genes and almost all of the HSI-III-associated genes. The downregulation of hcp2 and hcp3 transcription by 4CABA and 6CABA was further confirmed by qRT-PCR.
Formal Description
Interaction-ID: 28367

drug/chemical compound

6CABA

decreases_expression of

complex/PPI

T6SS-HSI-3 complex

shown in a microarray and qRT-PCR approach
Comment The mvfR inhibitors 4CABA (2-amino-4-chlorobenzoic acid) and 6CABA (2-amino-6-chlorobenzoic acid) repressed transcription of about half of the HSI-II-associated genes and almost all of the HSI-III-associated genes. The downregulation of hcp2 and hcp3 transcription by 4CABA and 6CABA was further confirmed by qRT-PCR.
Formal Description
Interaction-ID: 28368

drug/chemical compound

4CABA

decreases_activity of

gene/protein

mvfR

Comment The mvfR inhibitors 4CABA (2-amino-4-chlorobenzoic acid) and 6CABA (2-amino-6-chlorobenzoic acid) repressed transcription of about half of the HSI-II-associated genes and almost all of the HSI-III-associated genes. The downregulation of hcp2 and hcp3 transcription by 4CABA and 6CABA was further confirmed by qRT-PCR.
Formal Description
Interaction-ID: 28369

drug/chemical compound

6CABA

decreases_activity of

gene/protein

mvfR

Comment MvfR and LasR positively regulate hcp2 and hcp3 and negatively regulate hcp1.
Formal Description
Interaction-ID: 28370

gene/protein

mvfR

increases_expression of

gene/protein

hcp2

shown in qRT-PCR approach
Comment MvfR and LasR positively regulate hcp2 and hcp3 and negatively regulate hcp1.
Formal Description
Interaction-ID: 28371

gene/protein

lasR

increases_expression of

gene/protein

hcp2

shown in qRT-PCR approach
Drugbank entries Show/Hide entries for lasR
Comment MvfR and LasR positively regulate hcp2 and hcp3 and negatively regulate hcp1.
Formal Description
Interaction-ID: 28373

gene/protein

mvfR

increases_expression of

gene/protein

hcp3

shown in qRT-PCR approach
Comment MvfR and LasR positively regulate hcp2 and hcp3 and negatively regulate hcp1.
Formal Description
Interaction-ID: 28374

gene/protein

lasR

increases_expression of

gene/protein

hcp3

shown in qRT-PCR approach
Drugbank entries Show/Hide entries for lasR
Comment MvfR and LasR positively regulate hcp2 and hcp3 and negatively regulate hcp1.
Formal Description
Interaction-ID: 28375

gene/protein

mvfR

decreases_expression of

gene/protein

hcp1

shown in qRT-PCR approach
Comment MvfR and LasR positively regulate hcp2 and hcp3 and negatively regulate hcp1.
Formal Description
Interaction-ID: 28376

gene/protein

lasR

decreases_expression of

gene/protein

hcp1

shown in qRT-PCR approach
Drugbank entries Show/Hide entries for lasR