General Information:

Id: 3,011
Diseases: Pseudomonas aeruginosa diseases
pathogen-host system
Pseudomonas aeruginosa/mammalia
BTO:0000182 HT-29 cell, cocultured with P. aeruginosa strain PA103-delta-UT expressing plasmid-encoded ExoS-HA (Pa-ExoS-HA)
article
Reference: Bridge DR et al.(2010) Role of host cell polarity and leading edge properties in Pseudomonas type III secretion. Microbiology (Reading 156: 356-373 [PMID: 19910414]

Interaction Information:

Comment M-beta-CD (methyl-beta-cyclodextrin) depletes membrane cholesterol, and in dose-response studies, treatment of HT-29 epithelial cells with M-beta-CD, prior to co-culture with Pa-ExoS-HA, caused a decrease in T3S effector ExoS translocation.
Formal Description
Interaction-ID: 27476

drug/chemical compound

Methyl-beta-cyclodextrin

decreases transport of

gene/protein

exoS

Comment M-beta-CD (methyl-beta-cyclodextrin) depletes membrane cholesterol, and in dose-response studies, treatment of HT-29 epithelial cells with M-beta-CD, prior to co-culture with Pa-ExoS-HA, caused a decrease in T3S effector ExoS translocation.
Formal Description
Interaction-ID: 27485

drug/chemical compound

Methyl-beta-cyclodextrin

decreases_quantity of

cellular component

membrane raft

Comment Membrane fractionation studies confirmed that inhibition of Pa-T3S by M-beta-CD (methyl-beta-cyclodextrin) occurred at the level of Pa-T3S translocon insertion and effector translocation, as apparent in the decrease in PopB and ExoS in membrane fractions of M-beta-CD-treated cells.
Formal Description
Interaction-ID: 27486

drug/chemical compound

Methyl-beta-cyclodextrin

decreases_quantity of

gene/protein

popB

Comment Membrane cholesterol influences T3S translocon insertion.
Formal Description
Interaction-ID: 27487

cellular component

membrane raft

affects_folding of

complex/PPI

T3SS translocon complex

Comment Membrane fractionation studies confirmed that inhibition of Pa-T3S by M-beta-CD (methyl-beta-cyclodextrin) occurred at the level of Pa-T3S translocon insertion and effector translocation, as apparent in the decrease in PopB and ExoS in membrane fractions of M-beta-CD-treated cells.
Formal Description
Interaction-ID: 27488

drug/chemical compound

Methyl-beta-cyclodextrin

decreases_folding of

complex/PPI

T3SS translocon complex

Comment PFO (Perfringolysin O) affects membrane properties differently from M-beta-CD (methyl-beta-cyclodextrin), causing oligomerization of membrane cholesterol in conjunction with pore formation. PFO allowed translocon insertion but not effector translocation.
Formal Description
Interaction-ID: 27489

drug/chemical compound

Perfringolysin O

decreases transport of

gene/protein

exoS

Comment PFO (Perfringolysin O) affects membrane properties differently from M-beta-CD (methyl-beta-cyclodextrin), causing oligomerization of membrane cholesterol in conjunction with pore formation. PFO allowed translocon insertion but not effector translocation.
Formal Description
Interaction-ID: 27490

drug/chemical compound

Perfringolysin O

NOT decreases_quantity of

gene/protein

popB

Comment PFO (Perfringolysin O) affects membrane properties differently from M-beta-CD (methyl-beta-cyclodextrin), causing oligomerization of membrane cholesterol in conjunction with pore formation. PFO allowed translocon insertion but not effector translocation.
Formal Description
Interaction-ID: 27491

drug/chemical compound

Perfringolysin O

NOT decreases_folding of

complex/PPI

T3SS translocon complex

Comment PFO (Perfringolysin O) affects membrane properties differently from M-beta-CD (methyl-beta-cyclodextrin), causing oligomerization of membrane cholesterol in conjunction with pore formation. PFO allowed translocon insertion but not effector translocation.
Formal Description
Interaction-ID: 27492

drug/chemical compound

Perfringolysin O

affects_quantity of

cellular component

membrane raft

Comment Using Rac1 or Cdc42 inhibitors (NSC23766 or secramine A) to examine the role of focal complexes in T3S, both agents were found to inhibit T3S effector translocation. This was in contrast with ROCK or Src kinase inhibitors (Y-27632 or PP2) that target focal adhesions, which caused no significant inhibition of Pa-T3S.
Formal Description
Interaction-ID: 27493

gene/protein

RAC1

increases_transport of

gene/protein

exoS

Drugbank entries Show/Hide entries for RAC1
Comment Using Rac1 or Cdc42 inhibitors (NSC23766 or secramine A) to examine the role of focal complexes in T3S, both agents were found to inhibit T3S effector translocation. This was in contrast with ROCK or Src kinase inhibitors (Y-27632 or PP2) that target focal adhesions, which caused no significant inhibition of Pa-T3S.
Formal Description
Interaction-ID: 27494

gene/protein

CDC42

increases_transport of

gene/protein

exoS

Drugbank entries Show/Hide entries for CDC42
Comment Using Rac1 or Cdc42 inhibitors (NSC23766 or secramine A) to examine the role of focal complexes in T3S, both agents were found to inhibit T3S effector translocation. This was in contrast with ROCK or Src kinase inhibitors (Y-27632 or PP2) that target focal adhesions, which caused no significant inhibition of Pa-T3S.
Formal Description
Interaction-ID: 27495

gene/protein

ROCK1

NOT increases_transport of

gene/protein

exoS

Drugbank entries Show/Hide entries for ROCK1