General Information:

Id: 3,006 (click here to show other Interactions for entry)
Diseases: Pseudomonas aeruginosa diseases
pathogen-host system
Pseudomonas aeruginosa/mammalia
reporter system based on Bla/CCF2 enzyme/substrate combination
article
Reference: Verove J et al.(2012) Injection of Pseudomonas aeruginosa Exo Toxins into Host Cells Can Be Modulated by Host Factors at the Level of Translocon Assembly and/or Activity. PLoS ONE 7 [PMID: 22299042]

Interaction Information:

Comment To analyse T3SS translocon function in vivo a simple translocation reporter system based on the activity of beta--lactamase that cleaves a fluorescent substrate was used. The resulting chimeric proteins ExoS-Bla and ExoY-Bla were efficiently translocated into several cell lines such as the epithelial cell line A549, the B cell line BJAB, the T cell line Jurkat, and the differentiated promyelocytic HL-60 and promonocytic U937. No injection of the reporter fusion could be observed with undifferentiated HL-60 or U937 cells.
Formal Description
Interaction-ID: 27370

process

T3SS

increases_transport of

gene/protein

exoS

in A459, BJAB, Jurkat, differentiated HL-60 and U937 cells
Comment To analyse T3SS translocon function in vivo a simple translocation reporter system based on the activity of beta--lactamase that cleaves a fluorescent substrate was used. The resulting chimeric proteins ExoS-Bla and ExoY-Bla were efficiently translocated into several cell lines such as the epithelial cell line A549, the B cell line BJAB, the T cell line Jurkat, and the differentiated promyelocytic HL-60 and promonocytic U937. No injection of the reporter fusion could be observed with undifferentiated HL-60 or U937 cells.
Formal Description
Interaction-ID: 27372

process

T3SS

NOT affects transport of

gene/protein

exoS

in undifferentiated HL-60 or U937 cells
Comment Cholesterol-rich membrane microdomains have been implicated in invasion of P. aeruginosa. Depletion of cholesterol from lipid rafts by methyl-beta-cyclodextrin abolished the translocation of the reporter ExoS-Bla in a dose-dependent manner. Co-localisation of PopB with the lipid raft marker flotillin is further supporting the role of lipid rafts in the translocation process.
Formal Description
Interaction-ID: 27377

drug/chemical compound

Methyl-beta-cyclodextrin

decreases transport of

gene/protein

exoS

by cholesterol removal of cultured cells
Comment The actin network appears to play a major role in the translocation process in the VD3-differentiated-HL-60 model as evidenced by the action of cytochalasin D and latrunculin B, two actin-depolymerizing agents which were found to markedly inhibit the injection of ExoS-Bla.
Formal Description
Interaction-ID: 27378

affects transport of

gene/protein

exoS

Comment The actin network appears to play a major role in the translocation process in the VD3-differentiated-HL-60 model as evidenced by the action of cytochalasin D and latrunculin B, two actin-depolymerizing agents which were found to markedly inhibit the injection of ExoS-Bla.
Formal Description
Interaction-ID: 27379

drug/chemical compound

Cytochalasin D

decreases transport of

gene/protein

exoS

into VD3-dHL-60 cells
Comment The actin network appears to play a major role in the translocation process in the VD3-differentiated-HL-60 model as evidenced by the action of cytochalasin D and latrunculin B, two actin-depolymerizing agents which were found to markedly inhibit the injection of ExoS-Bla.
Formal Description
Interaction-ID: 27405

drug/chemical compound

Latrunculin B

decreases transport of

gene/protein

exoS

into VD3-dHL-60 cells
Drugbank entries Show/Hide entries for Latrunculin B
Comment Two specific PI3-kinase inhibitors, wortmannin and LY-294002 inhibited the translocation of ExoS-Bla, suggesting the involvement of PI3K signalling pathway in the translocon activity.
Formal Description
Interaction-ID: 27420

affects transport of

gene/protein

exoS

Comment Two specific PI3-kinase inhibitors, wortmannin and LY-294002 inhibited the translocation of ExoS-Bla, suggesting the involvement of PI3K signalling pathway in the translocon activity.
Formal Description
Interaction-ID: 27423

drug/chemical compound

Wortmannin

decreases transport of

gene/protein

exoS

into VD3-dHL-60 cells
Comment Two specific PI3-kinase inhibitors, wortmannin and LY-294002 inhibited the translocation of ExoS-Bla, suggesting the involvement of PI3K signalling pathway in the translocon activity.
Formal Description
Interaction-ID: 27425

drug/chemical compound

LY294002

decreases transport of

gene/protein

exoS

into VD3-dHL-60 cells
Comment ExoS-Bla injection into VD3-dHL-60 was abolished by genistein, a large spectrum tyrosine kinase inhibitor, by PP2, an inhibitor of the Src-kinase(s), and by PF-5732208, an inhibitor of focal adhesion tyrosine kinases, FAK and Pyk2.
Formal Description
Interaction-ID: 27427

drug/chemical compound

Genistein

decreases transport of

gene/protein

exoS

into VD3-dHL-60 cells
Drugbank entries Show/Hide entries for Genistein
Comment ExoS-Bla injection into VD3-dHL-60 was abolished by genistein, a large spectrum tyrosine kinase inhibitor, by PP2, an inhibitor of the Src-kinase(s), and by PF-5732208, an inhibitor of focal adhesion tyrosine kinases, FAK and Pyk2.
Formal Description
Interaction-ID: 27428

drug/chemical compound

PP2 (kinase inhibitor)

decreases transport of

gene/protein

exoS

into VD3-dHL-60 cells
Comment ExoS-Bla injection into VD3-dHL-60 was abolished by genistein, a large spectrum tyrosine kinase inhibitor, by PP2, an inhibitor of the Src-kinase(s), and by PF-5732208, an inhibitor of focal adhesion tyrosine kinases, FAK and Pyk2.
Formal Description
Interaction-ID: 27429

drug/chemical compound

PF-5732208

decreases transport of

gene/protein

exoS

into VD3-dHL-60 cells