General Information:

Id: 3,006 (click here to show other Interactions for entry)
Diseases: Pseudomonas aeruginosa diseases
pathogen-host system
Pseudomonas aeruginosa/mammalia
reporter system based on Bla/CCF2 enzyme/substrate combination
article
Reference: Verove J et al.(2012) Injection of Pseudomonas aeruginosa Exo Toxins into Host Cells Can Be Modulated by Host Factors at the Level of Translocon Assembly and/or Activity. PLoS ONE 7 [PMID: 22299042]

Interaction Information:

Comment To analyse T3SS translocon function in vivo a simple translocation reporter system based on the activity of beta--lactamase that cleaves a fluorescent substrate was used. The resulting chimeric proteins ExoS-Bla and ExoY-Bla were efficiently translocated into several cell lines such as the epithelial cell line A549, the B cell line BJAB, the T cell line Jurkat, and the differentiated promyelocytic HL-60 and promonocytic U937. No injection of the reporter fusion could be observed with undifferentiated HL-60 or U937 cells.
Formal Description
Interaction-ID: 27373

process

T3SS

NOT affects transport of

gene/protein

exoY

in undifferentiated HL-60 or U937 cells