General Information:

Id: 2,813
Diseases: Pseudomonas aeruginosa diseases
pathogen-host system
Pseudomonas aeruginosa/mammalia
BTO:0000018 A-549 cell, infected with P. aeruginosa strain PAO1
article
Reference: Wu M et al.(2011) Host DNA repair proteins in response to Pseudomonas aeruginosa in lung epithelial cells and in mice. Infect. Immun. 79: 75-87 [PMID: 20956573]

Interaction Information:

Comment The study demonstrates that P. aeruginosa infection causes severe DNA damage, which subsequently activates a group of DNA repair proteins (OGG1, APE1, and CSB).
Formal Description
Interaction-ID: 25923

process

P. aeruginosa infection

increases_activity of

Comment P. aeruginosa infection causes a commonly induced DNA damage adduct-oxoG (7,8-dihydro-8-oxoguanine). OxoG is the most common form of oxidative DNA adduct and tends to mispair with the base A (adenine), leading to a mutagenic transversion of GC to TA.
Formal Description
Interaction-ID: 25924

process

P. aeruginosa infection

increases_quantity of

drug/chemical compound

7,8-Dihydro-8-oxoguanine

Comment The study demonstrates that P. aeruginosa infection causes severe DNA damage, which subsequently activates a group of DNA repair proteins OGG1, APE1, and CSB.
Formal Description
Interaction-ID: 25927

process

P. aeruginosa infection

increases_activity of

gene/protein

OGG1

Comment The study demonstrates that P. aeruginosa infection causes severe DNA damage, which subsequently activates a group of DNA repair proteins OGG1, APE1, and CSB.
Formal Description
Interaction-ID: 25928

process

P. aeruginosa infection

increases_activity of

gene/protein

APEX1

Drugbank entries Show/Hide entries for APEX1
Comment The study demonstrates that P. aeruginosa infection causes severe DNA damage, which subsequently activates a group of DNA repair proteins OGG1, APE1, and CSB.
Formal Description
Interaction-ID: 25929

process

P. aeruginosa infection

increases_activity of

gene/protein

ERCC6

Comment OGG1 activity upon P. aeruginosa infection was significantly greater, 5-fold, than that in the controls. The expression of OGG1 was increased by more than 6-fold over the control as measured 1 h after P. aeruginosa infection.
Formal Description
Interaction-ID: 25930

process

P. aeruginosa infection

increases_quantity of

gene/protein

OGG1

Comment A549 cells overexpressing OGG1 exhibited stronger tolerance of PAO1 toxicity than did control cells. Overexpression of OGG1 significantly attenuated infection-induced DNA damage (reduced DNA strand breaks) compared to vector control.
Formal Description
Interaction-ID: 25931

gene/protein

OGG1

decreases_activity of

if OGG1 is overexpressed
Comment A549 cells overexpressing OGG1 exhibited stronger tolerance of PAO1 toxicity than did control cells. The results showed that overexpression of OGG1 significantly reduced the rate of cell death compared to that in vector control cells.
Formal Description
Interaction-ID: 25936

gene/protein

OGG1

decreases_activity of

process

cell death

if OGG1 is overexpressed
Comment Because live P. aeruginosa strain PAO1 bacteria induced higher levels of APE1 and CSB expression, these two repair proteins may significantly contribute to the in vivo host defense.
Formal Description
Interaction-ID: 25947

gene/protein

APEX1

affects_activity of

Drugbank entries Show/Hide entries for APEX1
Comment Because live P. aeruginosa strain PAO1 bacteria induced higher levels of APE1 and CSB expression, these two repair proteins may significantly contribute to the in vivo host defense.
Formal Description
Interaction-ID: 25948

gene/protein

ERCC6

affects_activity of

Comment Because live P. aeruginosa strain PAO1 bacteria induced higher levels of APE1 and CSB expression, these two repair proteins may significantly contribute to the in vivo host defense.
Formal Description
Interaction-ID: 25949

process

P. aeruginosa infection

increases_quantity of

gene/protein

APEX1

Drugbank entries Show/Hide entries for APEX1
Comment Because live P. aeruginosa strain PAO1 bacteria induced higher levels of APE1 and CSB expression, these two repair proteins may significantly contribute to the in vivo host defense.
Formal Description
Interaction-ID: 25964

process

P. aeruginosa infection

increases_quantity of

gene/protein

ERCC6

Comment siRNA-induced knock down of OGG1 corresponded with an increase in DNA damage due to PAO1 infection. There was a greater cytotoxicity in OGG1 siRNA-transfected cells than in control cells, suggesting that OGG1 may play a key role in counteracting bacterium-induced cytotoxic effects.
Formal Description
Interaction-ID: 25968

gene/protein

OGG1

decreases_activity of

process

P. aeruginosa infection

Comment P. aeruginosa infection causes a commonly induced DNA damage adduct-oxoG (7,8-dihydro-8-oxoguanine). OxoG is the most common form of oxidative DNA adduct and tends to mispair with the base A (adenine), leading to a mutagenic transversion of GC to TA.
Formal Description
Interaction-ID: 25994

increases_quantity of

drug/chemical compound

7,8-Dihydro-8-oxoguanine

Comment Both the delta-ExoS and delta-ExoT mutants showed less induction of OGG1 expression, with the reduction being much more pronounced in the delta-ExoS strain.
Formal Description
Interaction-ID: 25995

gene/protein

exoS

increases_quantity of

gene/protein

OGG1

delta-ExoS 6.5-fold versus delta-ExoT 2.6-fold reduction
Comment Both the delta-ExoS and delta-ExoT mutants showed less induction of OGG1 expression, with the reduction being much more pronounced in the delta-ExoS strain.
Formal Description
Interaction-ID: 25996

gene/protein

exoT

increases_quantity of

gene/protein

OGG1

delta-ExoS 6.5-fold versus delta-ExoT 2.6-fold reduction